Method for producing porcine pseudorabies gE gene deletion virus inactivated vaccine

A porcine pseudorabies, gene deletion technology, applied in the fields of biochemical equipment and methods, vaccines, viruses, etc., can solve the problems of lack of in-depth research, hidden dangers of vaccine safety, and long virus liquid time, so as to shorten production time and improve immunity. effect, the effect of improving growth activity
CN107137705AActive Publication Date: 2017-09-08广东渔跃生物技术有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广东渔跃生物技术有限公司
Publication Date
2017-09-08

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Abstract

The invention relates to a method for producing a porcine pseudorabies gE gene deletion virus inactivated vaccine by using a BHK cell line. The method comprises the following steps: thawing BHK21 cells, inoculating the cells into a spinner bottle for culturing until the cells grow into sheets, inoculating a porcine pseudorabies gE gene deletion virus, continuously culturing, collecting the cell venom when cytopathy CPE reaches 90% or higher, and concentrating, inactivating and degerming the collected venom, thereby obtaining a finished product. The porcine pseudorabies gE gene deletion virus liquor collected by the vaccine production method provided by the invention has high virus content of more than or equal to 107.2TCID50 / mL and has relatively high immunogenicity, an excellent immune effect can be achieved without adding any immunopotentiator, in-vivo secretion of neutralizing antibodies can be promoted after immunization, the immune protective rate reaches 100%, and the vaccine efficacy evaluation standard is completely met.
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Description

technical field

[0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a method for producing porcine pseudorabies gE gene-deleted virus inactivated vaccine with BHK cell line. Background technique

[0002] Porcine pseudorabies virus (PRV) belongs to herpesviridae aherpesviridae subfamily vesicular virus genus porcine herpesvirus type I, and pigs are the only natural host of the virus, causing pseudorabies in pigs. The disease is mostly outbreaks in pig herds, mainly harming sows, causing abortion or vertical transmission to piglets, resulting in a large number of deaths of newborn piglets, which has brought huge economic losses to the pig industry in my country and even the world.

[0003] Currently, there is no effective drug for the treatment of porcine pseudorabies, so vaccination has become the main measure to control the occurrence and prevalence of the disease. Currently widely used in the market is the nat...

Claims

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