Preparation method of decellularized amnion powder

Abstract

The invention discloses a preparation method of decellularized amnion powder. The preparation method comprises the following steps: 1) washing amnions and cutting the amnions into pieces; 2) washing the amnions in an oscillator at the temperature of 4 DEG C for 12-24h; 3) washing the amnions in the oscillator at the temperature of 4 DEG C with 1% of Triton x-100 and ammonium hydroxide for 1-3h; 4) washing the amnions in the oscillator at the temperature of 4 DEG C with deionized water for 12-24h and then washing the amnions with PBS (phosphate buffer solution) for 12-24h; 5) preserving the amnions at the temperature of -80 DEG C for 24h and freeze-drying; 6) grinding the amnions into powder in a low temperature environment; 7) storing the powder at the temperature of -80 DEG C after disinfection. According to the preparation method, cells of the amnions are eliminated by adopting a relatively mild decellularization method, disinfection treatment is performed, and the low temperature environment is strictly controlled in the whole process, so that active substances in the amnions are effectively protected; the prepared frozen amnion powder can be made into amnion glue or mist and the like, so that a wound surface can utilize collagen and protein micro-molecules more favorably, more effectively nutrition support is provided for healing of the wound surface, and the active substances of the amnion powder, such as growth factors, can sustainably promote epithelization.

Description

Technical field [0001] The invention relates to a method for preparing decellularized amniotic membrane powder. Background technique [0002] Amniotic membrane is the innermost layer of the placenta. It is a smooth, avascular, nerve and lymphatic, and elastic bio-semipermeable membrane that wraps amniotic fluid. Amniotic membrane contains type I, III, IV, V, VII collagen and fibronectin, Laminin and other protein components and some growth factors, such as epidermal growth factor (EGF), fibroblast growth factor (FGF), transforming growth factor (TGF), etc. These growth factors can promote the proliferation and migration of epithelial cells , Differentiation, enhance the adhesion of epithelial cells, promote epithelialization, and reduce scars; [0003] Amniotic membrane can promote the differentiation of conjunctival stem cells into conjunctival epithelial cells, promote the transformation of conjunctival epithelium to corneal epithelium, promote the proliferation of limbal stem c...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defects that the existing amniotic membrane decellularization method is not gentle enough, and has antigenicity and other risks derived from donors, and provides a method for preparing decellularized amniotic membrane powder that can adapt to different wounds