Preparation method of decellularized amnion powder

A technology of decellularization and amniotic membrane, which is applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc. It can solve the problems of the decellularization method is not gentle enough, and achieve the effect of short grinding time, small particle size and short action time

Active Publication Date: 2017-09-08
NOVAPRINT THERAPEUTICS SUZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defects that the existing amniotic membrane decellularization method is not gentle eno

Method used

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Embodiment Construction

[0029] The preferred embodiments of the present invention are described below. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0030] A method for preparing decellularized amniotic membrane powder is characterized in that it comprises the following steps:

[0031] 1) Collect fresh human placenta, peel the amniotic membrane from the inside of the placenta, wash the fresh amniotic membrane, remove blood stains on the surface, and cut into pieces; clean fresh amniotic membrane with sterile water, sterile saline or culture medium; the amniotic membrane can be washed Cut into pieces with an area of ​​5×5cm. You can use sterile scissors and tweezers, a scalpel or a slicer, or you can change the size according to your purpose; the fresh amniotic membrane is taken from the fresh placenta, which can be human or animal origin ;

[0032] 2) Wash with deionized...

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Abstract

The invention discloses a preparation method of decellularized amnion powder. The preparation method comprises the following steps: 1) washing amnions and cutting the amnions into pieces; 2) washing the amnions in an oscillator at the temperature of 4 DEG C for 12-24h; 3) washing the amnions in the oscillator at the temperature of 4 DEG C with 1% of Triton x-100 and ammonium hydroxide for 1-3h; 4) washing the amnions in the oscillator at the temperature of 4 DEG C with deionized water for 12-24h and then washing the amnions with PBS (phosphate buffer solution) for 12-24h; 5) preserving the amnions at the temperature of -80 DEG C for 24h and freeze-drying; 6) grinding the amnions into powder in a low temperature environment; 7) storing the powder at the temperature of -80 DEG C after disinfection. According to the preparation method, cells of the amnions are eliminated by adopting a relatively mild decellularization method, disinfection treatment is performed, and the low temperature environment is strictly controlled in the whole process, so that active substances in the amnions are effectively protected; the prepared frozen amnion powder can be made into amnion glue or mist and the like, so that a wound surface can utilize collagen and protein micro-molecules more favorably, more effectively nutrition support is provided for healing of the wound surface, and the active substances of the amnion powder, such as growth factors, can sustainably promote epithelization.

Description

Technical field [0001] The invention relates to a method for preparing decellularized amniotic membrane powder. Background technique [0002] Amniotic membrane is the innermost layer of the placenta. It is a smooth, avascular, nerve and lymphatic, and elastic bio-semipermeable membrane that wraps amniotic fluid. Amniotic membrane contains type I, III, IV, V, VII collagen and fibronectin, Laminin and other protein components and some growth factors, such as epidermal growth factor (EGF), fibroblast growth factor (FGF), transforming growth factor (TGF), etc. These growth factors can promote the proliferation and migration of epithelial cells , Differentiation, enhance the adhesion of epithelial cells, promote epithelialization, and reduce scars; [0003] Amniotic membrane can promote the differentiation of conjunctival stem cells into conjunctival epithelial cells, promote the transformation of conjunctival epithelium to corneal epithelium, promote the proliferation of limbal stem c...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L26/00A61K35/50A61K8/98A61P17/02A61Q19/00
CPCA61K8/982A61K35/50A61L26/0057A61L27/3604A61L27/3687A61L27/3691A61L2300/30A61L2300/412A61L2430/40A61Q19/00
Inventor 刘洪杨熙
Owner NOVAPRINT THERAPEUTICS SUZHOU CO LTD
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