Novel method for efficiently extractinganthocyanin from black rice

An anthocyanin and new method technology, applied in the field of food processing, can solve the problems of difficulty in popularization, low extraction efficiency, loss of anthocyanin, etc., and achieve the effects of protecting from damage, improving extraction yield, and promoting infiltration speed.

Inactive Publication Date: 2017-10-10
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to extract anthocyanins from plants, people have tried to extract anthocyanins by a variety of methods, such as solvent extraction, supercritical fluid extraction, microwave extraction, ultrasonic technology-assisted extraction, enzyme-assisted extraction, molecular distillation technology , two-phase extraction technology, microbial fermentation method, etc., but it is worth noting that the reported anthocyanin extraction methods have defects, and these defects include: 1) The extraction method is single and the extraction efficiency is low, such as solvent extraction; 2) Some extraction methods have high operational requirements and are difficult to popularize, such as supercritical fluid extraction, molecular distillation technology, two-phase extraction technology, microbial fermentation method, etc.; 3) extraction methods are expensive and difficult to popularize and apply, such as enzyme-assisted Extraction method, etc.; 4) Anthocyanin loss due to heat in the extraction process, such as microwave extraction method, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A new method for efficiently extracting anthocyanins from black rice comprises steps of drying black rice raw material, ultrafine pulverization, solvent immersion, ultra-high pressure extraction, filtration and centrifugation, removal of ethanol, ion exchange resin purification, and freeze-drying steps.

Embodiment 2

[0027] A new method for efficiently extracting anthocyanins from black rice, comprising the steps of drying black rice raw materials, ultrafine pulverization, solvent immersion, ultra-high pressure extraction, filtration and centrifugation, removal of ethanol, ion exchange resin purification, and freeze-drying steps; Ultra-high pressure extraction adopts intermittent pressurized ultra-high pressure extraction. The specific steps are: transfer the solvent mixture of dispersed black rice powder into a high-pressure polyethylene bag, exhaust as much air as possible and seal it, and transfer it to the ultra-high pressure extraction equipment. Extract under the condition of 250-350MPa for 10-30min, then release the pressure, maintain the normal pressure state for 5-10min, then extract under the condition of 350-450MPa for 10-30min, after the extraction, collect the extract for later use.

Embodiment 3

[0029] 1) Drying of black rice raw material: drying the black rice raw material at 40-45°C until its moisture content is 13%-14%;

[0030] 2) Superfine pulverization: pulverize the original black rice material in a high-speed pulverizer to a particle size of 80-120 mesh to obtain black rice powder;

[0031] 3) Solvent immersion: take a certain amount of black rice powder, add ethanol solution with a mass concentration of 50% according to the mass ratio of material to liquid of 1:5-15kg / kg, use hydrochloric acid to adjust the pH of the solution to 2.5-3.5, and stir evenly;

[0032] 4) Ultra-high pressure extraction: transfer the solvent mixture of the dispersed black rice powder into a high-pressure polyethylene bag, exhaust as much air as possible and seal it, transfer it to the ultra-high pressure extraction equipment, and extract under the condition of 250-350MPa for 10-30 minutes, Then release the pressure and maintain the normal pressure state for 5-10 minutes, and then ex...

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PUM

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Abstract

The inventiondiscloses a novel method for efficiently extractinganthocyanin from black rice. The method comprises steps of drying ofa black rice raw material, ultra-fine grinding, soaking in a solvent, ultra-high pressure extraction, filtration and centrifugation, ethanol removal, purification by ion exchange resin and freeze drying. The method comprises the following steps: firstly, black rice available in market or broken black rice formed during processing of the black rice is adopted as a raw material, and cells of the black rice are subjected to wall breaking treatment through superfine grinding; then, ultrahigh pressure assisted ethanol extraction is performed, and the ion exchange resin is combined for separation and purification. Extraction efficiency is high, the black rice anthocyanin with higher purity can be prepared, production cost is low, product stability is good, and popularization is facilitated.

Description

technical field [0001] The invention belongs to the technical field of food processing and relates to a new method for efficiently extracting anthocyanins from black rice. Background technique [0002] A large number of studies have shown that black rice anthocyanins are anthocyanin pigments, which belong to plant polyphenol compounds, which can reduce the incidence of coronary heart disease, improve visual acuity, antioxidant and anticancer activities, and can protect organisms from ultraviolet rays and ionizing radiation. , heavy metal pollution, low temperature and high temperature, etc. Black rice anthocyanins are not only a class of photoprotectants, anti-radiation agents, chelating agents, biological antioxidants and immune enhancers, but also a class of biosemiconductor materials. Studies in recent years have also found that black rice pigment has the functions of anti-venom, anti-cancer, inhibition of HIV replication, and treatment of Parkinson's disease. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 孙术国陈子钰林亲录杨涛周铁军曹建中丁玉琴
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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