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Biomimetic injectable adhesive hydrogel, preparation method thereof, and application thereof to biological aspect

A hydrogel and adhesive technology, applied in applications, drug delivery, pharmaceutical formulations, etc., to achieve good material safety, easy preparation and operation, and pain relief

Inactive Publication Date: 2017-11-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the continuous improvement of people's quality of life and exercise level, there are more and more injuries to tendons, nerves and other tissues. The current treatment methods mainly include surgical treatment and conservative treatment, but all of them have greater or lesser risks. Therefore, we urgently need a biocompatible and adhesive material to help it repair and solve some problems, so the biomimetic adhesive hydrogel is used in many polymers with medical functions due to its unique biochemical functions and physical properties. Standing out from the biomaterials and endowing them with more new functions without losing their own characteristics will make this material play an important role in biomedicine and other fields.

Method used

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  • Biomimetic injectable adhesive hydrogel, preparation method thereof, and application thereof to biological aspect
  • Biomimetic injectable adhesive hydrogel, preparation method thereof, and application thereof to biological aspect
  • Biomimetic injectable adhesive hydrogel, preparation method thereof, and application thereof to biological aspect

Examples

Experimental program
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Effect test

Embodiment 1

[0017] 1) Weigh 1.0 g of sodium hyaluronate (700 kDa) and dissolve it in 150 mL of deionized water with stirring at room temperature, and add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride ( EDC) 1.4260g, N-hydroxysuccinimide (NHS) 0.8561g, after stirring rapidly for 30 minutes, add dopamine hydrochloride 0.9404g, obtain colorless and transparent liquid after completely dissolving, then use concentration as 0.4mol / L The pH of the system was adjusted to 5.0 with hydrochloric acid, and after passing nitrogen gas for 5 minutes, the mixture was stirred for 20 hours under airtight conditions. Then use a dialysis bag with a molecular weight of 14,000 to dialysis in deionized water for 3 days under the condition of nitrogen flow, and change the water 5 times. Porous sample A.

[0018] 2) Weigh 0.10g of sample A into a 10mL glass bottle, add 4.90mL deionized water to make a 2.0wt% aqueous solution, and prepare 10mM H 2 o 2 Aqueous solution, horseradish peroxidase (HRP)...

Embodiment 2

[0020] 1) Weigh 1.0g of sodium alginate, stir and dissolve it in 200mL of deionized water at room temperature, and add 2.852g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in sequence , N-hydroxysuccinimide (NHS) 1.7122g, add dopamine hydrochloride 1.9124g after rapid stirring for 30 minutes, obtain colorless and transparent liquid after completely dissolving, then use the hydrochloric acid adjustment system of concentration to be 0.4mol / L When the pH reached 4.7, nitrogen gas flowed for 5 minutes, and the mixture was stirred for 20 hours under airtight conditions. Then use a dialysis bag with a molecular weight of 8000 to dialysis in deionized water for 3 days under the condition of nitrogen flow, and change the water 5 times. Porous sample B.

[0021] 2) Weigh 0.10g of sample B into a 10mL glass bottle, add 4.90mL deionized water to make a 2.0wt% aqueous solution, and configure 20mM H 2 o 2 Aqueous solution, horseradish peroxidase (HRP) aqueous solu...

Embodiment 3

[0023] 1) Weigh 1.0g of pectin, stir and dissolve it in 150mL of deionized water at room temperature, add 5.7038g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) successively, N-hydroxysuccinimide (NHS) 3.4244g, after stirring rapidly for 30 minutes, add 2.7112g of dopamine hydrochloride, and obtain a colorless and transparent liquid after completely dissolving, then adjust the pH of the system with hydrochloric acid with a concentration of 0.4mol / L To 5.0, after nitrogen flow for 5 minutes, airtight stirring was carried out for 20 hours. Then, use a dialysis bag with a molecular weight of 14000 to dialysis in deionized water for 2 days under the condition of nitrogen flow, and change the water 4 times. Porous sample C.

[0024] 2) Weigh 0.10g of sample C into a 10mL glass bottle, add 4.90mL deionized water to make a 2.0wt% aqueous solution, and configure 10mM H 2 o 2 Aqueous solution, mushroom polyphenol oxidase aqueous solution of 100u / mL, phosphate b...

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Abstract

The invention relates to biomimetic injectable adhesive hydrogel, a preparation method thereof, and application thereof to the biological aspect, and belongs to the technical field of macromolecular materials. Firstly, natural macromolecular hyaluronic acid is modified by dopamine and the product still has high water solubility; then horse radish peroxidase and hydrogen peroxide are added, the pH is adjusted to be alkalescent, mixing is conducted uniformly through oscillation, the process of changing from sol to gel is generated, the color is changed from colorless to brown, and the adhesive property is given to the hydrogel. In addition, the original biocompatibility is high, the characteristics such as non-toxicity and degradability are retained, the performance conservation is further proved via cytotoxicity test and in-vitro degradation test, and with reference to the characteristics, the biomimetic injectable adhesive hydrogel has the potential to be a novel biological material showing capabilities in the field of biomedicine such as cell and tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a bionic injectable adhesive hydrogel, a preparation method and its biological application. Background technique [0002] In recent years, with the rapid development of frontier disciplines and the rapid development of science and technology, bionic materials have been widely used in military materials, biomedical materials, communication materials and other aspects. Biomimetic materials inherit the unique structure and superior performance of natural biological materials, and people aim to prepare new biomimetic materials with different functions. At present, the research on biomimetic materials has achieved remarkable results in terms of structure and function, and hydrogel has also become one of the beneficiaries. Hydrogel is a kind of material with a three-dimensional polymer network structure. The coexistence of hydrophilic and hydrophobic groups makes...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08J3/075C08J3/24A61L24/00A61L24/08A61L24/10A61L26/00A61L27/20A61L27/22A61L27/52A61L27/56C08B37/08C08B37/04C08B37/06
CPCA61L24/0031A61L24/0036A61L24/08A61L24/108A61L26/0023A61L26/0047A61L26/008A61L26/0085A61L27/20A61L27/227A61L27/52A61L27/56A61L2400/06C08B37/0045C08B37/0072C08B37/0084C08J3/075C08J3/24C08J9/28C08J2201/0484C08J2207/10C08J2207/12C08J2305/04C08J2305/06C08J2305/08C08L5/04C08L5/06C08L5/08C08L89/00
Inventor 林权刘厚赵月赵玥琪张川杨雪杨柏
Owner JILIN UNIV
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