Preparation method of rice husk oligosaccharide
A technology of rice husk oligosaccharides and rice husks, which is applied in the field of preparation of rice husk oligosaccharides, can solve the problems of high water consumption in the treatment process, low yield of oligosaccharides, and difficult wastewater treatment, and achieve low cost and high water consumption , the effect of promoting rapid growth and reproduction
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Embodiment 1
[0020] Embodiment 1, a kind of preparation method of rice husk oligosaccharide, the steps are as follows:
[0021] (1) Crush the rice husk to 70 mesh, and treat it for 8 hours in an environment with a temperature of 0°C and a pressure of 1800 bar;
[0022] (2) After the treated rice husk is fully steamed and sterilized in steam, it is dried until the water content is 70%, and after cooling down to normal temperature, 10 parts of glucose, 0.01 part of ferrous sulfate, 0.02 part of thiamine, 0.01 part of riboflavin, 0.01 part of nicotinic acid, 0.01 part of dopamine hydrochloride, 0.01 part of sodium iodide and mix well;
[0023] (3) Inoculate cellulase into the rice husk, the inoculation ratio is 3‰, enzymatic hydrolysis at 35°C, and the enzymatic hydrolysis time is 38 hours;
[0024] (4) Mix the rice husk obtained by enzymatic hydrolysis with 3 times the volume of water evenly, introduce it into a high-speed centrifuge, and heat the surface of the centrifuge at the same time,...
Embodiment 2
[0027] Embodiment 2, a kind of preparation method of rice husk oligosaccharide, the steps are as follows:
[0028] (1) Crush the rice husk to 75 mesh, and treat it for 9 hours in an environment with a temperature of -1°C and a pressure of 1900 bar;
[0029] (2) After the treated rice husk is fully steamed and sterilized in steam, it is dried to a water content of 71%, and after cooling down to normal temperature, 11 parts of glucose, 0.012 parts of ferrous sulfate, 0.021 parts of thiamine, 0.011 parts of riboflavin, 0.012 parts of nicotinic acid, 0.0102 parts of dopamine hydrochloride, 0.0102 parts of sodium iodide and mix well;
[0030] (3) Cellulase was inoculated into the rice husk, the inoculation ratio was 4‰, enzymolysis was carried out at 36°C, and the enzymolysis time was 39 hours;
[0031] (4) Mix the rice husk obtained by enzymatic hydrolysis with 4 times the volume of water evenly, introduce it into a high-speed centrifuge, and heat the surface of the centrifuge at...
Embodiment 3
[0034] Embodiment 3, a kind of preparation method of rice husk oligosaccharide, the steps are as follows:
[0035] (1) Crush the rice husk to 80 mesh, and treat it for 10 hours in an environment with a temperature of -2°C and a pressure of 2000 bar;
[0036] (2) After the treated rice husk is fully steamed and sterilized in steam, it is dried to a water content of 72%, and after cooling down to normal temperature, 12 parts of glucose, 0.015 part of ferrous sulfate, 0.022 part of thiamine, 0.013 part of riboflavin, 0.012 part of nicotinic acid, 0.0103 part of dopamine hydrochloride, 0.0103 part of sodium iodide and mix well;
[0037] (3) Inoculate cellulase into the rice husk, the inoculation ratio is 5‰, enzymatic hydrolysis at 38°C, and the enzymatic hydrolysis time is 40 hours;
[0038] (4) Mix the rice husk obtained by enzymatic hydrolysis with 5 times the volume of water evenly, introduce it into a high-speed centrifuge, and heat the surface of the centrifuge at the same ...
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