Special mixed-strain microbial fertilizer for onobrychis viciifolia and preparation method thereof
A technology of microbial fertilizer and mixed strains, applied in the field of agricultural fertilizers, can solve the problems such as difficulty in effectively preventing and controlling the ecological environment of soil-borne diseases, incomplete and balanced nutrient absorption, and lowering efficiency of chemical fertilizer use, so as to overcome the decline in the quality of agricultural products and achieve excellent physical and chemical properties. , the effect of improving the ability to resist repeated stubble
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Embodiment 1
[0035] Example 1 Composite bacterial agent and single strain bacterial agent use effect comparison
[0036] (1) Preparation of bacterial agent
[0037] Preparation of single-strain bacterial agent: prepare NA liquid medium, inoculate three strains of Bacillus mycoides Gnyt1, Pseudomonas sp. After 24 to 48 hours, use a hemocytometer to count the number of bacteria in the culture medium under a microscope. When the number exceeds 10 9 When the cfu / mL was reached, the culture was terminated and the single-strain inoculum of each strain was obtained.
[0038] Preparation of compound bacterial agent: Mix each single-strain bacterial agent according to the ratio of 1:1:1 to obtain the mixed bacterial agent.
[0039] (2) Determination of each index
[0040] 1) Determination of phosphorus
[0041] The phosphorus-dissolving ability (that is, the effective phosphorus increment in the bacterial solution, including organic phosphorus and inorganic phosphorus) was measured by the MBC m...
Embodiment 2
[0067] Embodiment 2 Preparation and Fertilizer Efficiency Experiment of Special Mixed Strain Microbial Fertilizer for Ormosia edulis
[0068] The invention provides a special mixed strain microbial fertilizer for red bean grass. According to parts by weight, its raw material components include 25 parts of charcoal powder, 25 parts of flower soil, 14 parts of decomposed cow dung, 10 parts of straw powder, and fulvic acid. 4 parts of potassium, 2 parts of rare earth, 5 parts of red bean grass root nodule agent, 5 parts of Bacillus mycoides Gnyt1 strain fermentation broth, 5 parts of Pseudomonas sp.CHO19 strain fermentation broth, Bacillus subtilis FX2 strain 5 parts of fermentation broth.
[0069] The preparation method of above-mentioned mixed bacterial strain microbial fertilizer is as follows:
[0070] (1) Cow dung is added with water and stacked for retting, heat-preserved and fermented, and dried in the sun after making it decomposed;
[0071] (2) After removing impuritie...
experiment example
[0078] Experimental example: the special mixed bacterial strain microbial fertilizer of red bean grass prepared in embodiment 2
[0079] Control group: no fertilization treatment was used as the control
[0080] Experimental method: put the seeds on plastic cloth before sowing, and then spray an appropriate amount of sterile water on the seeds to make the seeds moist, then take the biological fertilizer and sprinkle it on the seeds, and stir while spreading until evenly mixed, per mu The usage amount is 2.0kg. After drying in the shade, they can be sown. Harvest at the best cutting time, determine the content of crude protein in the hay, and count the annual hay production.
[0081] Experimental time: The experiment started in March 2014, continued for 3 years (2014-2016), and collected data for three years.
[0082] Experimental site: The experimental site is located in Tongwei County, Dingxi City, Gansu Province
[0083] Experimental method and results: The field test was ...
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