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Reagent and method for quantitatively detecting full-scale C-reactive protein in whole blood

A technology of reactive protein and detection reagents, which is applied in the field of detection reagents, can solve the problems of not mentioning the detection of full-scale CRP, and the absence of quantitative detection reagents for full-scale CRP in whole blood, etc., to achieve a wide range of clinical applications, less sample demand, Apply a wide range of effects

Inactive Publication Date: 2018-02-02
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent does not mention whether it can detect the full range of CRP
After inquiry, no whole blood full range CRP quantitative detection reagent has been found so far

Method used

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  • Reagent and method for quantitatively detecting full-scale C-reactive protein in whole blood
  • Reagent and method for quantitatively detecting full-scale C-reactive protein in whole blood
  • Reagent and method for quantitatively detecting full-scale C-reactive protein in whole blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A full range of C-reactive protein detection reagents in whole blood, including reagent R1 and reagent R2.

[0043] Specifically:

[0044] Reagent R1 includes: cell-breaking components, buffer, surfactant, electrolyte, preservative, reaction accelerator, and stabilizer. Wherein: the cell-breaking component is a fatty acid salt with a mass percentage of 0.05%, the buffer solution is a glycine buffer solution with a pH value of 5.0 and a concentration of 30mmol / L, the surfactant is Tween20 with a mass percentage of 0.01%, and the electrolyte is a mass percentage The sodium salt is 0.1%, the preservative is sodium azide with a mass percentage of 0.15%, the reaction accelerator is polyethylene glycol 2000 with a mass percentage of 0.20%, and the stabilizer is bovine serum albumin with a mass percentage of 0.50%.

[0045] Reagent R2 includes: anti-human C-reactive protein sensitized latex particles, surfactants, stabilizers, preservatives, electrolytes, and buffers. Wherei...

Embodiment 2

[0050] A full range of C-reactive protein detection reagents in whole blood, including reagent R1 and reagent R2.

[0051] Specifically:

[0052] Reagent R1 includes: cell-breaking components, buffer, surfactant, electrolyte, preservative, reaction accelerator, and stabilizer. Wherein: the broken cell component is the sulfate ester salt of 1.50% by mass percent, the buffer solution is the Tris buffer solution with a pH value of 10.0 and a concentration of 50mmol / L, and the surfactant is Emulgen B-66 with 3.00% by mass percent, The electrolyte is 1.5% potassium salt by mass percentage, the preservative is 2.00% by mass sorbate, the reaction accelerator is 3.00% by mass of polyethylene glycol 4000, and the stabilizer is 2.00% by mass of gelatin.

[0053] Reagent R2 includes: anti-human C-reactive protein sensitized latex particles, surfactants, stabilizers, preservatives, electrolytes, and buffers. Wherein: the mixing ratio of large latex particles and small latex particles i...

Embodiment 3

[0058] A full range of C-reactive protein detection reagents in whole blood, including reagent R1 and reagent R2.

[0059] Specifically:

[0060] Reagent R1 includes: cell-breaking components, buffer, surfactant, electrolyte, preservative, reaction accelerator, and stabilizer. Wherein: the broken cell component is the phosphate ester salt of 0.50% by mass percentage, the buffer solution is the phosphoric acid-citric acid buffer solution with a pH value of 7.0 and a concentration of 50mmol / L, and the surfactant is Span20 with 1.00% by mass percentage, The electrolyte is 1.00% by mass percent sodium salt, the preservative is 1.00% by mass percent gentamicin, the reaction accelerator is 1.00% by mass percent polyethylene glycol 8000, and the stabilizer is 1.00% by mass percent of ethylene glycol.

[0061] Reagent R2 includes: anti-human C-reactive protein sensitized latex particles, surfactants, stabilizers, preservatives, electrolytes, and buffers. Among them: the mixing rati...

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Abstract

The invention discloses a reagent for detecting a full-scale C-reactive protein in whole blood. The detection reagent includes a reagent R1 and a reagent R2, the reagent R1 is a reagent for quantitatively detecting hemoglobin, and the detection result is used for hematocrit correction of the C-reactive protein concentration of the whole blood, and the reagent R1 and the reagent R2 are combined toobtain the reagent for detecting the full-scale C-reactive protein. A detection sample used by the reagent for quantitatively detecting the full-scale C-reactive protein in whole blood can be peripheral blood and other blood. The detection reagent can meet the requirements of determination of the content of high-concentration CRP and determination of the content of low-concentration CRP, has the advantages of high sensitivity, strong stability, wide linear range, good anti-interference ability, and good accuracy and precision, and can meet clinical examination requirements. The invention alsodiscloses a method for quantitatively detecting the full-scale C-reactive protein in the whole blood.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a reagent for quantitative detection of C-reactive protein in whole blood. The invention also relates to a method for detecting full range C-reactive protein in whole blood. Background technique [0002] C-reactive protein (C-reactive protein, CRP) is a positive acute phase reaction protein, which is secreted by the liver when the body is infected or tissue damaged, rises sharply within a few hours, and drops rapidly during the recovery period of the disease, so It has important clinical significance in the early diagnosis, course and prognosis of infection, inflammation and other diseases. On the other hand, CRP is an independent predictor of cardiovascular disease, that is, high-sensitive C-reactive protein (hs-CRP). The American Heart Association recommended in 2003: hs-CRP<1mg / L is low risk, 1-3mg / L is medium risk,>3mg / L is high risk. According to the dete...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/68G01N33/54313G01N2333/4737
Inventor 林清菁范华范翠翠王剑顾裕峰
Owner PUREBIO LAB NINGBO
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