Phomopsis asparagi LAMP (loop-mediated isothermal amplification) detection primer pair and detection method thereof

A technology for stem blight of asparagus and stem blight of asparagus, applied in biochemical equipment and methods, recombinant DNA technology, measurement/inspection of microorganisms, etc., can solve cumbersome detection and identification procedures, high requirements for identification experience, and low accuracy and other problems, to achieve the effect of good practicability, simple and fast operation, and high accuracy
CN107699634AInactive Publication Date: 2018-02-16INST OF PLANT PROTECTION FAAS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PLANT PROTECTION FAAS
Publication Date
2018-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a phomopsis asparagi LAMP (loop-mediated isothermal amplification) detection primer pair and a detection method thereof, which are specially used for specific detection of phomopsis asparagi, and belongs to the field of disease detection and biological technologies for crops. The phomopsis asparagi LAMP detection primer pair comprises outer primers F3 and B3 and inner primers FIP and BIP and has the sequence in SEQ ID NO.1-4. According to the phomopsis asparagi detection method established on the basis of the primer pair, green fluorescence or ladder bands with the LAMPfeatures can be observed through a chromogenic reaction or agarose gel electrophoresis detection after LAMP constant-temperature amplification. According to the LAMP detection primer pair and the detection method thereof, rapid, sensitive and accurate detection of plants infected with phomopsis asparagi can be realized in production practice, besides, the LAMP detection primer pair and the detection method can be used for early diagnosis of field diseases and monitoring and identification of germs and reliable technological and theoretical bases are provided for early warning and control of the phomopsis asparagi.
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Description

technical field

[0001] The invention relates to a LAMP detection primer set of asparagus stem blight and a detection method thereof, which is specially used for rapid molecular detection of asparagus stem blight, and can realize early diagnosis of asparagus stem blight and monitoring and identification of pathogens, and belongs to crop diseases Detection, identification, control and biotechnology fields. Background technique

[0002] Asparagus is rich in saponins, sterols, flavonoid glycosides, isoflavones, sulfur-containing amino acids, histones, nucleic acid and enzyme macromolecules, vitamin C, vitamin E, B vitamins, rutin, folic acid, carbohydrate immune activators and Manganese, zinc, copper, iron and other trace minerals necessary for human health have extremely high nutritional value and are a kind of precious health-care vegetables. The nutritious and healthy high-end vegetables that are loved by consumers enjoy the reputation of "King of Vegetables" internationally, ...

Claims

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