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Device, system, preparation method, target content determination method and kit for detecting target ion content

A technology for detecting target and ion content, which is applied in the field of devices for detecting target ion content, can solve the problems of large sampling volume, difficult to meet, and easy to be interfered by external electromagnetic fields, and achieves the effect of reducing the measurement error of the result and improving the accuracy.

Active Publication Date: 2021-04-06
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ion-selective electrode is a kind of electrochemical sensor that uses membrane potential to measure the activity or concentration of ions in solution. The ion activity is directly related to the membrane potential. Using this type of electrode to measure the target ion content is accurate, fast, and cheap, but it is accompanied by the need for frequent maintenance, calibration of electrode response, blood coagulation prevention during sample delivery, and the monitoring process is easily interfered by external electromagnetic fields. , and large volume, inconvenient to carry, and large sampling volume, not suitable for small sample analysis, etc.
[0005] The ion-selective optode is usually a polymer sensing membrane with carrier support or the newly proposed paper-based ion sensor. Due to the different ion concentrations of the sample, it will be combined with protonated sensor molecules in different degrees through proton exchange. state, and finally show different colors, judge the color of the polymer film or paper or measure its spectral signal with the naked eye, and determine the concentration of ions in the sample. Background interference, and the need to control the pH, so the sample requirements are high, often need to be diluted, centrifuged, adjusted pH and other pretreatments before the test, the cost of manpower and equipment is high, the detection speed is slow, and it is difficult to meet the convenience of life analysis and the rapid determination of outpatient and emergency departments needs

Method used

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  • Device, system, preparation method, target content determination method and kit for detecting target ion content
  • Device, system, preparation method, target content determination method and kit for detecting target ion content
  • Device, system, preparation method, target content determination method and kit for detecting target ion content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] 5 mg of potassium ionophore with a mass fraction of 14%, 3 mg of ion exchanger tetrakis (3,5-bis(trifluoromethyl) phenyl) sodium borate of 8%, chromogenic ionophore I of 1 mg of 3% and 21% of the 8mg amphiphilic block homopolymer Pluronic F-127 and 54% of the 20mg parent material bis(2-ethylhexyl) sebacate were dissolved in 0.1mL / mg of tetrahydrofuran respectively to obtain a mixed solution; Add the solution to 50 times the volume of phosphate buffer with a pH of 7.4, shake repeatedly until the system reaches equilibrium, and remove THF by reducing pressure to obtain the dispersion of potassium ion nanosensors, see figure 1 .

[0086] The particle diameter of the nanosensor in the dispersion liquid of the potassium ion nanosensor was measured by dynamic light scattering to be 90±2nm, and the PDI was 0.12±0.02.

[0087] After testing, the particle size of the nanosensor thus prepared has the characteristics of small particle size and uniform distribution. Due to the sma...

Embodiment 2

[0089] In the dispersion of the potassium ion nanosensor prepared in Example 1, add about 1.0% agarose by mass ratio, heat to 95 degrees Celsius to dissolve the agarose, and place the resulting hot-dissolved solution in a cuvette, After cooling to room temperature, the potassium ion-selective nano-sensing hydrogel is obtained. see figure 2 The photo taken by TEM transmission electron microscope of the nano-sensing hydrogel. The nano-sensing hydrogel thus prepared is colorless, transparent and stable in performance. It can sense changes in ion concentration in the external environment through ion diffusion and display different colors. It has a wide range of application values ​​in the field of detection and analysis.

[0090] The preparation method of the nano-sensing hydrogel is simple, and large-scale production can be realized, which is suitable for industrial and commercial production requirements.

Embodiment 3

[0091] Embodiment 3 (measurement method based on distance)

[0092] To the nano-sensing hydrogel prepared in Example 2, add the potassium ion standard solution with a concentration of 0.01, 0.003, 0.001, and 0.0003mol / L respectively, so that the standard solution is fully in contact with the gel, and after 2 minutes, in the vertical In the direction of potassium ions entering the gel, use the camera to capture the image of the hydrogel at each standard concentration, and analyze the color change in the same period of time, see image 3 (a), calculate the color change distance, establish the standard curve that the color change distance is linearly related to the potassium ion concentration, see image 3 (b).

[0093] Add whole blood to the device prepared in Example 2, make the whole blood fully contact with the gel, and after 2 minutes, use a camera to capture the image of the hydrogel in the direction perpendicular to the direction that potassium ions enter the gel, and ana...

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Abstract

The invention provides a device for detecting the concentration of target ions and its application. According to an embodiment of the present invention, the method includes: a gel body, the gel body is formed by a gel and a dispersion medium; and a nano sensor, the nano sensor is arranged in the gel body, and the nano The sensor is selective for the target ion. According to the embodiment of the present invention, due to the large number of micropores in the gel, only small molecules in the sample are allowed to pass through in the form of diffusion, and have the function of filtering the macromolecules in the sample. When the nano-sensing hydrogel is in contact with the sample, it can Effectively prevent the entry of blood cells, proteins, etc., making the reagent less susceptible to the interference of sample color background and scattering, the sample does not need to be pre-treated, and can measure whole blood; reduce the measurement error of the result and improve the accuracy of the content determination.

Description

technical field [0001] The invention relates to the fields of analysis and medical detection. Specifically, the present invention relates to a device, a system, a preparation method, a method for measuring the content of a target, and a kit for detecting the content of a target ion. Background technique [0002] Inorganic salt ions such as sodium ions, potassium ions, and calcium ions are important substances to maintain the normal physiological functions of organisms. Their concentration or content in the human body is often in a relatively stable equilibrium state, so the determination of these ions in human blood and urine The content in them has become an important indicator to measure the health status of organisms, and it also has guiding significance for the formulation and production monitoring of food and medicine. [0003] At present, the above-mentioned ion content determination methods commonly used mainly include the determination of potentiometric analysis bas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 谢小江杨竹根
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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