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Method for producing biological energy source by microalgae via two-step method

A bio-energy and microalgae technology, applied in microorganisms, microorganism-based methods, and treatment of microorganisms with electricity/wave energy, etc., can solve the problems of long route, high cost, unsuitable for large-scale industrialization, etc., and achieve the calorific value of combustion. High, low cost, suitable for large-scale industrialization promotion

Inactive Publication Date: 2018-03-27
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the world's energy consumption, biomass energy has accounted for 14%. Using microalgae to produce high-purity ethanol, methanol, butane and other energy compounds, the greatest use of microalgae is that its stem cells contain more than 70% of microalgae oil , is the best raw material for subcritical biotechnology to synthesize biodiesel, and is an ideal renewable energy source. At present, the process route for producing bioenergy from microalgae is long and the cost is high, so it is not suitable for large-scale industrialization.

Method used

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  • Method for producing biological energy source by microalgae via two-step method

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Embodiment 1

[0022] A two-step method for producing bioenergy from microalgae, comprising the following steps:

[0023] (1) Chlorella is inoculated on the medium, and the medium is composed of the following parts by weight: 250 parts of sodium nitrate, 350 parts of urea, 120 parts of potassium dihydrogen phosphate, 50 parts of magnesium sulfate, calcium chloride 60 parts, 0.2 parts of cobalt nitrate hexahydrate, 0.2 parts of disodium edetate, 0.05 parts of gum arabic, 0.03 parts of seaweed polysaccharide, 0.03 parts of carrageenan, 0.002 parts of trace elements and 6000 parts of tourmaline activated water. The base was illuminated for 11 hours a day but direct light should be avoided. The light intensity was 400 Lux. The culture medium was placed in an atmosphere with a volume ratio of carbon dioxide to oxygen of 10:1 and cultivated in a magnetic field. The culture temperature was 27°C, and the magnetic field strength was Periodic changes, first increase from 0A / m at a rate of 3A / m / min to ...

Embodiment 2

[0026] A two-step method for producing bioenergy from microalgae, comprising the following steps:

[0027] (1) Golden algae is inoculated on the medium, and the medium is composed of the following parts by weight: 200 parts of sodium nitrate, 300 parts of urea, 100 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate, 30 parts of calcium chloride 0.1 part of cobalt nitrate hexahydrate, 0.1 part of disodium edetate, 0.02 part of gum arabic, 0.01 part of seaweed polysaccharide, 0.01 part of carrageenan, 0.001 part of trace elements and 5000 parts of tourmaline activated water. Illumination is 10 hours a day but direct light should be avoided. The light intensity is 300 Lux. The culture medium is placed in an atmosphere with a volume ratio of carbon dioxide to oxygen of 10:1 and cultivated in a magnetic field. The culture temperature is 25°C and the magnetic field strength is cycled Sexual change, first increased from 0A / m to 30A / m at a rate of 2A / m / min, and the...

Embodiment 3

[0030] A two-step method for producing bioenergy from microalgae, comprising the following steps:

[0031] (1) Inoculate Nannochloropsis on the culture medium, and the culture medium is composed of the following parts by weight: 300 parts of sodium nitrate, 400 parts of urea, 150 parts of potassium dihydrogen phosphate, 80 parts of magnesium sulfate, chloride 80 parts of calcium, 0.4 parts of cobalt nitrate hexahydrate, 0.4 parts of disodium edetate, 0.08 parts of gum arabic, 0.05 parts of seaweed polysaccharide, 0.05 parts of carrageenan, 0.005 parts of trace elements and 8000 parts of tourmaline activated water. The culture medium is illuminated for 12 hours a day but direct light should be avoided. The light intensity is 500 Lux. The culture medium is placed in an atmosphere with a volume ratio of carbon dioxide to oxygen of 10:1 and cultivated in a magnetic field. The culture temperature is 30°C and the magnetic field strength Do periodic changes, first increase from 0A / m ...

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Abstract

The invention provides a method for producing biological energy source by microalgae via a two-step method, and relates to the field of biological energy source. The method comprises the following steps of (1) inoculating the microalgae onto a culture medium, radiating the culture medium by light for 10 to 12h each day, avoiding direct light radiating, placing the culture medium into an atmosphere, and culturing in a magnetic field, wherein the volume ratio of carbon dioxide to oxygen in the atmosphere is equal to 10:1; (2) harvesting the microalgae, airing, grinding into powder, adding hot water and phosphoric acid, stirring, centrifuging, sucking a supernatant into a container, using a sodium hydroxide solution to adjust the pH (potential of hydrogen) value, adding a mixed solution and an adsorbent, heating, stirring, performing suction filtration and separation, extracting a water phase by the mixed solution for two times, combining organic phases, drying, relieving pressure and concentrating, so as to obtain the biological energy source. The method has the advantages that the technology route is short, the cost is low, and the method is suitable for large-scale industrialized popularization; the prepared biological energy source is clean and environmentally friendly, and the combustion calorific value is high.

Description

technical field [0001] The invention relates to the field of bioenergy, in particular to a two-step method for producing bioenergy by microalgae. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them useful in the fields of food, medicine, genetic engineering, and liquid fuels. Very good development prospects. The esters and glycerol rich in microalgae are good raw materials for preparing liquid fuels; the calorific value of biomass fuel produced by microalgae is 1.4-2 times that of wood or crop straw. In the world's energy consumption, biomass energy has accounted for 14%. Using microalgae to produce high-purity ethanol, methanol, butane and other energy compounds, the greatest use of microalgae is that its stem cells contain more than 70% of microalgae oil , is the best raw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12P7/64C12R1/89
CPCC12N13/00C12P7/6463
Inventor 陈天仁王丽娟杨宋琦杨生辉
Owner HEXI UNIV