Corn seed size gene ZmUrb2, expression product, clone primer and expression vector thereof and application

A technology of grain size and expression product, which is applied in the field of maize genetics and breeding to achieve the effects of increasing maize yield, improving grain shape and improving growth period

Active Publication Date: 2018-03-30
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, at present, Chinese scientists have limited research on the biological functions of genes related to corn kernel shape. It is urgent to discover excellent allelic variation, explore its regulatory mechanism and interaction network, and provide technical support for improving corn yield.

Method used

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  • Corn seed size gene ZmUrb2, expression product, clone primer and expression vector thereof and application
  • Corn seed size gene ZmUrb2, expression product, clone primer and expression vector thereof and application
  • Corn seed size gene ZmUrb2, expression product, clone primer and expression vector thereof and application

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Experimental program
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Effect test

Embodiment 1

[0042] Embodiment one: a kind of corn kernel size mutant type ks1 discovery

[0043] In February 2009, at the Southern Propagation Base of Henan Agricultural University in Ledong County, Hainan Province, Professor Tang Jihua of Henan Agricultural University was in the process of breeding corn breeding materials, and found that after selfing of the material numbered 6243, some grains appeared as small grains and wrinkled ears. The phenotype mutation of shrunken kernels, the material with this phenotype mutation is named ks1 . Such as figure 1 Parts A, B, C, F, G, and H are shown in: ks1 The mature grains of the mutants showed smaller grains, different degrees of reduction in grain length, grain width and grain thickness, and a decrease in hundred-grain weight. ks1 The serial number is 6244.

[0044] F to 6244×B73 1 , F 2 and BC 1 Investigation and analysis of the size separation of panicles and grains, the results are as follows figure 1 As shown in Part A and Ta...

Embodiment 2

[0050] Embodiment two: a kind of corn kernel size mutant type ks1 Regulatory Gene Discovery

[0051] 1. Select 400 pairs of SSR markers evenly covering 10 chromosomes of the corn genome from the maize database maizegdb, and screen the markers that are closely linked with the target gene and have polymorphisms between the two parents and the constructed BSA pool, among which only 1.02 bins are screened Two polymorphic markers bnlg1803 and bnlg1007 were found, indicating that the target gene is located on the first chromosome;

[0052] 2. Screened the SSR primers near the 1.02 region, that is, covering 1.01, 1.02, and 1.03 bins, and screened a total of 7 pairs of polymorphic markers;

[0053] 3. Use the screened polymorphic markers to carry out genetic linkage analysis on each individual plant in the preliminary positioning population, screen the exchanged individual plants, and analyze the direction of the target gene;

[0054] 4. Referring to the mapping method proposed by ...

Embodiment 3

[0062] Embodiment three: a kind of corn kernel size mutant type ks1 Identification of Regulatory Genes

[0063] 15 days after pollination, wild-type and mutant endosperm RNA was extracted to semi-quantify each candidate gene, such as Figure 4 Shown:

[0064] There was a significant difference in the expression of the No.1 gene between the wild type and the mutant type, the overall expression of the No.2 gene was low, and there was no significant difference in the expression of the No.3 gene and the No.4 gene between the wild type and the mutant endosperm;

[0065] The four candidate gene segments were sequenced and analyzed in the wild-type and mutant materials, and it was found that there was a specific single-base mutation site SNP22 in the coding region of the No. 1 gene, the base C was mutated to T, corresponding to the coding The amino acid was mutated from normal leucine to proline. Further sequencing analysis proved that this site is relatively conserved in natural ...

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Abstract

The invention discloses a corn seed size gene ZmUrb2, an expression product, clone primer and expression vector thereof and application. The corn seed size gene ZmUrb2, the expression product, clone primer and expression vector thereof and the application have the advantages that the regulating mechanism of a corn seed size natural mutant material is analyzed through various methods, the gene ZmUrb2 for regulating the corn seed size and growth period is cloned, the function of the gene ZmUrb2 is verified, the beneficial haplotype of the gene ZmUrb2 discovered, and a foundation is laid for theapplication practice of corn breeding; the mutation of the ZmUrb2 can reduce the sensitivity of the mutant material to specific antibiotics, pre-rRNA processing lag can be caused to allow growth and development to have evident defects, the optimal haplotype, with high gene expression quantity, high corn seed length and short growth period, of the ZmUrb2 segment is screened out from 479 tropical, subtropical and temperate natural corn population materials, the haplotype can be used for improving the corn seed type and growth period, corn yield increasing is benefited, and the tropical corn materials can be favorably improved and planted in the temperate zone.

Description

technical field [0001] The invention belongs to the technical field of corn genetics and breeding, in particular to a corn grain size gene ZmUrb2 , its expression product, its cloning primer, its expression vector and its application. Background technique [0002] High and stable grain yield is an important goal of plant science research. Corn is not only the main food source, but also an important feed and industrial raw material, which plays a very important role in the development of the national economy. As the population increases and the arable land decreases, the status of corn will become more and more important. [0003] The number of grains per ear, grain weight and number of ears per unit area are the basic elements of maize yield. The number of grains per ear is determined by the number of rows of ears and the number of grains per row, and the weight of grains is determined by the size of the grains and the bulk density. "Grain type" not only includes the size...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C12N15/82C07K14/415A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 汤继华王洪秋付志远李文学郭战勇张雪海康定明
Owner HENAN AGRICULTURAL UNIVERSITY
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