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A kind of Citrobacter strain and its application

A technology of citric acid bacteria and citric acid, applied in the field of environmental microorganisms, to achieve stable removal effect, high bacterial activity and strong adaptability

Active Publication Date: 2020-12-08
江苏博恩环境工程成套设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the removal of hydrogen sulfide and ammonia by Citrobacter

Method used

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  • A kind of Citrobacter strain and its application
  • A kind of Citrobacter strain and its application
  • A kind of Citrobacter strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment is the screening and identification of deodorizing strain ZT-S5:

[0033] 1. Cultivate

[0034] The medium used in the following experiments was sterilized at 121°C for 20 minutes during the preparation process.

[0035] Luria-Bertani medium (LB): tryptone 10.0g, sodium chloride (NaCl) 10.0g, yeast extract 5.0g, agar powder 15.0g, add distilled water to 1000mL, pH=7.4, sterilize at 121°C for 20min. Its 12-hour growth curve in LB is as follows figure 1 As shown, the strain can grow to the maximum concentration on LB medium after 18 hours.

[0036] Beef extract-peptone medium (NA): 3.0g beef extract, 5.0g sodium chloride (NaCl), 10.0g peptone, 15.0g agar powder, add distilled water to 1000mL, pH=7.5±0.1, sterilize at 121°C for 20min.

[0037] Ammonia enriched medium: 5.0 g sucrose, 2.0 g peptone, potassium dihydrogen phosphate (KH 2 PO 4 ) 2.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.5g, sodium chloride (NaCl) 2.0g, zinc sulfate (ZnSO 4 ) 0.05g, ferr...

Embodiment 2

[0063] In this example, strain ZT-S5 is used for deodorization in the whole process.

[0064] The deodorized wastewater sample is the untreated sewage of Nanjing Chengbei Sewage Treatment Plant. Since ammonia and hydrogen sulfide are mainly produced by the decomposition of organic matter by microorganisms under anaerobic conditions, the experimental condition is to add inoculum to the static sewage. Deodorize the activated sludge of the strain, and measure the content of ammonia and hydrogen sulfide in the sewage at different times, and calculate the removal rate.

[0065] The specific process is:

[0066] (1) Add the ZT-S5 bacterial solution cultivated for 12 hours into the sludge, and stir evenly. The control group is the culture medium without adding bacteria and is added to the sludge according to the same inoculum amount.

[0067] (2) Pack the uniformly stirred sludge into large test tubes, fill it to 1 / 5 of the test tube, and fill the test tube with sewage. The CFU coun...

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Abstract

The invention belongs to the field of environmental microorganisms, and relates to a Citrobacter strain and an application thereof. The name of the Citrobacter sp. is deodorizing bacteria ZT‑S5, and it was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on December 25, 2017. The strain preservation number is CGMCC No.15120. The Citrobacter strain provided by the invention has high activity and strong adaptability, and can be widely used in the biological treatment process of hydrogen sulfide and ammonia in water bodies and gases. The biological deodorization device made by absorbing the strain can well resist the impact of waste gas and realize the application of biological deodorization.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and relates to a Citrobacter strain and an application thereof. Background technique [0002] With the continuous development of the economy, the continuous advancement of the urbanization process, and the continuous improvement of people's living standards, municipal treatment facilities such as sewage treatment plants, sewage lifting stations, garbage transfer stations, and landfills are getting closer and closer to people's living areas. The malodorous exhaust gas produced during the operation of these facilities has become an important factor affecting people's normal life. Malodorous exhaust gas mainly includes sulfur-containing compounds, nitrogen-containing compounds, halogens and their derivatives, hydrocarbons, oxygen-containing organics, organic acids, etc. [0003] The malodorous gas takes the air as the transmission medium and causes harm to human health through the respir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/58B01D53/52C12R1/01
CPCB01D53/52B01D53/58B01D53/84C02F3/34C02F2303/02C12N1/205C12R2001/01Y02A50/20
Inventor 蔡泉生傅小强刘玉安
Owner 江苏博恩环境工程成套设备有限公司
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