Targeting atherosclerotic focus cell membrane bionic drug-delivery system preparation method and product and application thereof
A technology for atherosclerosis and drug delivery system, which is applied in the field of pharmacy to achieve good in vivo and in vitro targeting effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] Example 1. Preparation and Characterization of Macrophage Membrane Biomimetic Nanocarriers
[0030] (1) Extraction of macrophage cell membrane:
[0031] Refer to the preparation method of Cao H et al. (Cao H, Dan Z, He X, et al. Liposomes Coated with Isolated Macrophage Membrane Can Target Lung Metastasis of Breast Cancer [J]. Acs Nano, 2016, 10:7738-7748.). Collect macrophages, add 4℃TM buffer (pH 7.4, 0.01mol L -1 Tris and 0.001mol·L -1 MgCl) was resuspended, and the concentration of the cell suspension was adjusted to 2.5×10 7 / mL, squeeze the cell membrane back and forth with a micro-homogenizer (20 times), add an appropriate amount of 1mol L -1 Sucrose was mixed with cell homogenate to make the final concentration of sucrose 0.25mol L -1 . Then proceed as follows:
[0032] 1) Centrifuge at 2000g for 10min at 4°C, and take the supernatant;
[0033] 2) Centrifuge at 3000g for 30min at 4°C, discard the supernatant;
[0034] 3) Add 0.25mol·L at 4°C -1 Resuspe...
Embodiment 2
[0039] Example 2, establishment and evaluation of human umbilical vein endothelial cell injury model
[0040] (1) Conditions for establishment of human umbilical vein endothelial cell inflammatory injury model:
[0041] Take HUVEC cells in the logarithmic growth phase, and use 1×10 per well 4 / mL density inoculated in 96-well plate, added 100 μL to each well, and adhered to the wall overnight; aspirated the medium, added serum-free medium to starve for 12 hours, and gave different concentrations of LPS (final concentrations were 0, 1.56, 3.125, 6.25, 12.5, 25 , 50, 100, 200μg·mL -1 ), three replicate wells in each group, placed in CO 2 The culture was continued in the incubator; 20 μL of MTT solution (5 g·L -1 ), 4 hours later, 150 μL of dimethyl sulfoxide was added to each well, and the absorbance (A) value was measured at a wavelength of 490 nm with a microplate reader after shaking at 37° C. for 30 minutes.
[0042] (2) α4β1 integrin and VCAM-1 protein expression detect...
Embodiment 3
[0048] Example 3. Preliminary evaluation of in vivo targeting of macrophage membrane biomimetic nanocarriers
[0049] (1) Establishment of male SPF grade ApoE in atherosclerosis model - / - Gene knockout mice and C57BL / 6J mice were fed with high-fat diet (78% basal diet+21% fat+1.25% cholesterol) for 12 weeks respectively.
[0050] (2) Evaluation of atherosclerosis model
[0051] The model mice were taken and injected intraperitoneally with 3% sodium pentobarbital (60 mg·kg -1 ). After the mouse is anesthetized, put it on an ice pack and dissect the thorax. Use a 1mL syringe to quickly collect 0.8-1mL of blood from the left ventricle, quickly transfer it to an EP tube, centrifuge at 3500g, 4°C for 15min, separate the plasma, and store at 4°C. Store in the refrigerator for later use. After fully perfused with pre-cooled PB buffer and 4% paraformaldehyde (flow rate 2mL·min -1 ), the thoracic aorta (starting from the root of the heart to the branches of the bilateral iliac art...
PUM
| Property | Measurement | Unit |
|---|---|---|
| The average particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap