Preparation method of probiotic fermented strawberry fruit powder, and prepared probiotic fermented strawberry fruit powder
A strawberry fruit and probiotic technology, which is applied to the preservation of fruits and vegetables, the function of food ingredients, and the heating and preservation of fruits/vegetables, etc. The effect of group balance, convenient storage, and extended shelf life
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[0030] A preparation method of probiotic fermented strawberry fruit powder includes the following steps:
[0031] Probiotic fermentation: Take 100 parts of sterilized, enzyme-inactivated strawberry pulp in parts by mass, and add 100-200 parts of sterile water, 0.001-5 parts of probiotic starter and 1- 10 parts of sugar, mixed evenly, transferred to 37-40°C for 60-72h fermentation, filtered to obtain probiotic fermented strawberry pulp;
[0032] Drying: The fermented strawberry pulp adopts low-temperature vacuum drying method to remove water to obtain probiotic fermented grass berry powder;
[0033] Wherein, the probiotic starter is Bacillus coagulans, or the probiotic starter is a mixed starter composed of at least one of Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus plantarum and Bacillus coagulans;
[0034] The sugar agent includes at least one of fructose syrup, dextrose powder, dissolved white sugar and honey.
[0035] Further, the sterilization method of the straw...
Example Embodiment
[0046] Example 1
[0047] Step 1: Preparation of probiotic seed solution
[0048] The freeze-dried bacterial powders of Bacillus coagulans, Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus plantarum were respectively inoculated into the MRS solid medium and transferred and activated for three generations. Then the activated strains were inoculated into MRS liquid medium for expansion culture, and the cells were collected by centrifugation, and finally suspended in 0.85% normal saline to prepare a seed solution for later use.
[0049] Step 2: Prepare starter
[0050] A skimmed milk emulsion with a concentration of 10% by mass was prepared, and after sterilization, it was inoculated with a probiotic seed solution at a volume ratio of 5% and cultured at 37°C. Regularly test the viable counts of the 4 strains of probiotic fermentation broth, when the viable counts in each fermentation broth reach 10 8 -10 10 At CFU / mL, stop the culture and prepare a mature starter.
[0051]...
Example Embodiment
[0069] Example 2
[0070] In this example, the first step: preparing the probiotic seed liquid, the second step: preparing the starter, and the third step: preparing the powder freeze-dried strains are the same as those in Example 1.
[0071] The fourth step: preparation of compound probiotic starter
[0072] Take 2g of Bacillus coagulans powder, 2g of Lactobacillus plantarum powder, 2g of Lactobacillus casei powder and 2g of Lactobacillus rhamnosus according to the weight of each powder freeze-dried strain, and mix them thoroughly to prepare a composite probiotic starter for fermentation. Strawberry puree. Determine the total number of viable bacteria in the compound probiotic starter by gradually diluting it to ensure that the total number of viable bacteria in the compound probiotic starter is greater than or equal to 10 8 Pieces / g.
[0073] Step 5: Preparation of probiotic fermented strawberry fruit powder
[0074] (1) Preparation of strawberry pulp
[0075] Select 1000g fresh stra...
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