Preparation method of probiotic fermented strawberry fruit powder, and prepared probiotic fermented strawberry fruit powder
A strawberry fruit and probiotic technology, which is applied to the preservation of fruits and vegetables, the function of food ingredients, and the heating and preservation of fruits/vegetables, etc. The effect of group balance, convenient storage, and extended shelf life
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preparation example Construction
[0030] A preparation method of probiotic fermented strawberry fruit powder, comprising the following steps:
[0031] Probiotic fermentation: in parts by mass, take 100 parts of sterilized and enzyme-inactivated strawberry pulp, add 100-200 parts of sterile water, 0.001-5 parts of probiotic starter and 1- 10 parts of sugar preparation, mixed evenly, transferred to 37-40°C for fermentation for 60-72 hours, filtered to obtain probiotic fermented strawberry pulp;
[0032] Drying: the fermented strawberry pulp adopts low-temperature vacuum drying method to remove moisture, and obtains probiotic fermented strawberry fruit powder;
[0033] Wherein, the probiotic starter is Bacillus coagulans, or the probiotic starter is a mixed starter composed of at least one of Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus plantarum and Bacillus coagulans;
[0034] The sugar preparation includes at least one of fructose syrup, glucose powder, dissolved white granulated sugar and h...
Embodiment 1
[0047] Step 1: Prepare Probiotic Seed Solution
[0048] The freeze-dried bacterial powders of Bacillus coagulans, Lactobacillus rhamnosus, Lactobacillus casei, and Lactobacillus plantarum were respectively inoculated into MRS solid medium, and then activated for three generations. Then the activated bacteria were inoculated in the MRS liquid medium to expand the culture, and the bacteria were collected by centrifugation, and finally suspended with 0.85% physiological saline, and prepared as seed liquid for later use.
[0049] Step 2: Prepare the starter
[0050] Prepare skimmed milk emulsion with a concentration of 10% by mass percentage, inoculate probiotic seed liquid with a volume ratio of 5% after sterilization, and cultivate at 37°C. Regularly detect the number of viable bacteria in the fermentation liquid of 4 strains of probiotics, when the number of viable bacteria in each fermentation liquid reaches 10 8 -10 10 When the CFU / mL is reached, the culture is stopped and...
Embodiment 2
[0070] In this example, the first step: preparation of probiotic seed liquid, the second step: preparation of starter and the third step: preparation of powder freeze-dried strains are all the same as in Example 1.
[0071] Step 4: Prepare the compound probiotic starter
[0072] According to the weight of each powder freeze-dried strain, take Bacillus coagulans powder 2g, Lactobacillus plantarum powder 2g, Lactobacillus casei powder 2g and Lactobacillus rhamnosus 2g, mix well and make a compound probiotic starter for fermentation Strawberry puree. The compound probiotic starter is measured for the total number of live bacteria wherein by a stepwise dilution method to ensure that the total number of live bacteria in the composite probiotic starter is greater than or equal to 10 8 pcs / g.
[0073] Step 5: Prepare probiotic fermented strawberry fruit powder
[0074] (1) Preparation of strawberry pulp
[0075] Select 1000g of fresh strawberry fruit with moderate ripeness, good ...
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