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A method for separating and purifying euglena species

A technology of separation and purification, Euglena algae liquid, applied in microorganism-based methods, biochemical equipment and methods, separation of microorganisms, etc., can solve problems such as susceptible bacteria, and achieve the effect of stable traits

Active Publication Date: 2022-02-01
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the long screening time of euglena species and the problem that it is very easy to infect bacteria in the process of subculture and large-scale cultivation, the present invention provides a low-cost and easy-to-operate algae purification procedure by using measures such as acidification and light induction, so that To achieve the purpose of quickly obtaining pure species of euglena and adapting to large-scale euglena production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Natural water samples. In the field sampling, a large amount of water in the growth of Euglena cells, the dominant species. 500mL samples from the waters.

[0037] (1) back to the lab, the water sample is added sterile flask, nutrient access, the formula is: Ammonium nitrate 1g / L, potassium dihydrogen phosphate 0.2g / L, magnesium sulfate 0.1g / L, calcium chloride 0.1g / L, sodium citrate, 1g / L, VB 1 10μg / L, VB 12 0.1μg / L, ferric chloride, 0.588mg / L, manganese chloride 0.108mg / L, zinc sulfate 0.078mg / L, cobalt chloride, 0.012mg / L, sodium molybdate 0.0075mg / L; adjusted with 1M HCl in the above algae solution pH of the intensity 3.5,3000lx, 12h / d of light, into the CO-containing 2 A mixed gas of 5% (0.3 vvm) were cultured, the culture temperature was 25 ± 2 ℃, incubation time 3d.

[0038] (2) microscopic examination of samples and preliminary estimates algal cell concentration, was diluted with sterile water concentration estimation according to the algae...

Embodiment 2

[0046] Natural water sample. Sampling in the wild, naked algae cells, naked algae cells, certain green algae and diatoms are superior algae strains in a certain water. Sampling 500ml from this water.

[0047] (1) Back to the laboratory, add water sample to a sterile triangulation, add nutrient salt: 1 g / L of ammonium nitrate, 0.2 g / L of phosphate, 0.1 g / l, calcium chloride 0.1g / L, sodium citrate 1g / L, VB 1 10μg / L, VB 12 0.1μg / L, iron chloride 0.58 mg / L, chloride 0.108 mg / L, zinc sulfate 0.078 mg / L, cobalt chloride 0.012 mg / L, sodium molybdate 0.0075 mg / L; Adjust the above The algae pH is 3.5, 3000LX light intensity, 12H / D light, access to CO 2 5% of the mixed gas (0.3 Vm) was cultured, the culture temperature was 25 ± 2 ° C, and the culture time was 3d. Sampling detection found that there are still more green algae and diatoms, and naked algae cells are basically superior algae (about 50%). For the convenience of test, the algae pH is 3, continue to culti...

Embodiment 3

[0055] Natural water sample. Sampling in the wild, naked algae cells in a certain water, but superior algae is green and diatom. Sampling 500ml from this water.

[0056] (1) Back to the laboratory, add water sample to a sterile triangulation, add nutrient salt: 1 g / L of ammonium nitrate, 0.2 g / L of phosphate, 0.1 g / l, calcium chloride 0.1g / L, sodium citrate 1g / L, VB 1 10μg / L, VB 12 0.1μg / L, iron chloride 0.58 mg / L, chloride 0.108 mg / L, zinc sulfate 0.078 mg / L, cobalt chloride 0.012 mg / L, sodium molybdate 0.0075 mg / L; Adjust the above The algae pH is 3.5, 3000LX light intensity, 12H / D light, access to CO 2 5% of the mixed gas (0.3 Vm) was cultured, the culture temperature was 25 ± 2 ° C, and the culture time was 3d. Sampling detection found that there were still more green algae and diatoms, and naked algae cells were basically superior algae (about 30%). For the convenience of test, the algae liquid pH is 3, continued to cultivate 4D, at this time The alg...

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Abstract

The invention discloses a method for separating and purifying euglena species. It selects water samples containing euglena cells or euglena liquid containing miscellaneous bacteria, adds a certain amount of inorganic medium suitable for the growth of euglena, and then adjusts the euglena The pH of the liquid is 3-4, and the light intensity of 3000-30000lx is cultivated for 2-7d; then, the algae liquid is diluted, put into a specific container, and 2000-5000lx light is applied to a certain part of the culture device for a certain period of time. A certain amount of algae liquid is sucked into the illuminated part and inserted into the culture well plate for cultivation. In this way, algal species with higher purity can be obtained in a short period of time, the growth cycle is shortened, and the success rate of large-scale growth is improved.

Description

Technical field [0001] The present invention relates to microalgal species purification techniques, and in particular relates to a method for separation and purification of algae Euglena. Background technique [0002] Euglena living in fresh water, often forming green blooms in lakes and rivers, especially the organic matter-rich waters are more common, form a green film of water in the water, and with the outside world of light and other changes in form or spread. Euglena without cell walls, has a long flagellum, flagella and twisting motion through the cell, chloroplast, may rely on intracellular autotrophic photosynthesis, but also organic material may be utilized (e.g., glucose, glutamic acid, malic acid, pyruvic acid , lactic acid and ethanol) camps heterotrophic, usually optical in nature while autotrophic and heterotrophic. Euglena elongated rod-shaped body, width is generally 10-20 m, a length of up to 100 m; eye having a point, more sensitive to light (phototaxis / dark ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 王兆伟彭小伟王六伟
Owner NINGBO FUTIAN BIOTECH
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