Injectable bone repair material with controllable magnesium ion release behavior and preparation method of material

Abstract

The invention discloses an injectable bone repair material with a controllable magnesium ion release behavior and a preparation method of the material, relates to the field of injectable bone repair materials, and aims at developing an injectable microsphere cell carrier loaded with magnesium-containing inorganic components. Different from common microcarriers such as sephadex in a large-scale cell culture technology, microphseres are prepared from aliphatic polyester with adjustable rate of biodegradation and can be injected into defect parts in a human body together with cells performing adhesive growth on the surfaces by serving as cell carriers, and along with tissue regeneration, a polyester microsphere support is gradually degraded and absorbed by an organism and gives way to regenerated tissue. The magnesium-containing inorganic components are introduced in the microcarriers, by selecting different magnesium-containing inorganic components and changing the proportion and intakeof the magnesium-containing inorganic matter in the microspheres, the release behavior of magnesium ions is regulated and controlled, and then the bioactive microsphere type bone repair material withthe excellent osteogenesis promoting capacity is developed by means of the biological effect of the magnesium ions in the new bone formation process.

Description

technical field

[0001] The invention relates to the field of injectable bone repair materials, in particular to a microsphere injectable bone repair material with controllable magnesium ion release behavior and a preparation method thereof. Background technique

[0002] Bone defect is a common clinical surgical disease. Compared with autologous bone transplantation, allogeneic bone transplantation and xenograft bone transplantation, bone tissue engineering has opened up a new research field for the clinical repair of bone defects, and is expected to solve the problems of traditional implant repair. Faced with problems such as limited sources and immune rejection. Scaffold materials play a very important role in the application of tissue engineering principles to repair bone defects, not only as a carrier for cell adhesion and proliferation, but also to provide bioactive factors to promote osteogenic differentiation of cells.

[0003] Aliphatic polyesters such as polylactic ...

Examples

Embodiment 1

[0029] (1) 1 gram of lactide-glycolide copolymer (PLGA7525, lactide / glycolide molar ratio 75:25, molecular weight 50,000) was dissolved in 20 milliliters of dichloromethane solution, and magnetically stirred for 12 hours to obtain Solution A.

[0030] (2) Add 20 mg of Span 80 and 0.05 g, 0.1 g or 0.2 g of magnesium oxide powder to solution A, and homogenize at 12000 rpm for 5 minutes to obtain dispersion B.

[0031] (3) Dissolve 0.2 gram of Tween 60 and 2 gram of polyvinyl alcohol (PVA 1788) in 200 milliliters of deionized water to obtain solution C, and add the dispersion prepared in step (2) dropwise to this solution C under stirring at 300 rpm B. After the dropwise addition, the stirring was continued at room temperature for 8 hours to volatilize the dichloromethane solvent.

[0032] (4) Stop stirring, centrifuge at 4000rpm for 10 minutes to collect hardened microspheres, wash 3 times with deionized water, and freeze-dry for 24 hours to obtain magnesium oxide and PLGA weig...

Embodiment 2

[0034] (1) 1 gram of lactide-glycolide copolymer (PLGA7525, lactide / glycolide molar ratio 75:25, molecular weight 50,000) was dissolved in 20 milliliters of dichloromethane solution, and magnetically stirred for 12 hours to obtain Solution A.

[0035] (2) Add 20 mg of Span 80 and 0.05 g, 0.1 g or 0.2 g of magnesium carbonate powder to solution A, and homogenize at 12000 rpm for 5 minutes to obtain dispersion B.

[0036] (3) Dissolve 0.2 gram of Tween 60 and 2 gram of polyvinyl alcohol (PVA 1788) in 200 milliliters of deionized water to obtain solution C, and add the dispersion prepared in step (2) dropwise to this solution C under stirring at 300 rpm B. After the dropwise addition, the stirring was continued at room temperature for 8 hours to volatilize the dichloromethane solvent.

[0037] (4) Stop stirring, centrifuge at 4000rpm for 10 minutes to collect hardened and formed microspheres, wash 3 times with deionized water, and freeze-dry for 24 hours to obtain magnesium carb...

Embodiment 3

[0039] (1) 1 gram of lactide-glycolide copolymer (PLGA7525, lactide / glycolide molar ratio 75:25, molecular weight 50,000) was dissolved in 20 milliliters of dichloromethane solution, and magnetically stirred for 12 hours to obtain Solution A.

[0040] (2) Add 20 mg of Span 80, and 0.2 g of magnesium oxide and magnesium carbonate powder to solution A, the mass ratio of magnesium oxide to magnesium carbonate is 3:1, 1:1, 1:3, homogeneous at 12000rpm After 5 minutes, dispersion B was obtained.

[0041] (3) Dissolve 0.2 gram of Tween 60 and 2 gram of polyvinyl alcohol (PVA 1788) in 200 milliliters of deionized water to obtain solution C, and add the dispersion prepared in step (2) dropwise to this solution C under stirring at 300 rpm B. After the dropwise addition, the stirring was continued at room temperature for 8 hours to volatilize the dichloromethane solvent.

[0042] (4) Stop stirring, centrifuge at 4000rpm for 10 minutes to collect hardened microspheres, wash 3 times wit...

technical field

[0001] The invention relates to the field of injectable bone repair materials, in particular to a microsphere injectable bone repair material with controllable magnesium ion release behavior and a preparation method thereof. Background technique

[0002] Bone defect is a common clinical surgical disease. Compared with autologous bone transplantation, allogeneic bone transplantation and xenograft bone transplantation, bone tissue engineering has opened up a new research field for the clinical repair of bone defects, and is expected to solve the problems of traditional implant repair. Faced with problems such as limited sources and immune rejection. Scaffold materials play a very important role in the application of tissue engineering principles to repair bone defects, not only as a carrier for cell adhesion and proliferation, but also to provide bioactive factors to promote osteogenic differentiation of cells.

[0003] Aliphatic polyesters such as polylactic ...

Examples

Embodiment 1

[0029] (1) 1 gram of lactide-glycolide copolymer (PLGA7525, lactide / glycolide molar ratio 75:25, molecular weight 50,000) was dissolved in 20 milliliters of dichloromethane solution, and magnetically stirred for 12 hours to obtain Solution A.

[0030] (2) Add 20 mg of Span 80 and 0.05 g, 0.1 g or 0.2 g of magnesium oxide powder to solution A, and homogenize at 12000 rpm for 5 minutes to obtain dispersion B.

[0031] (3) Dissolve 0.2 gram of Tween 60 and 2 gram of polyvinyl alcohol (PVA 1788) in 200 milliliters of deionized water to obtain solution C, and add the dispersion prepared in step (2) dropwise to this solution C under stirring at 300 rpm B. After the dropwise addition, the stirring was continued at room temperature for 8 hours to volatilize the dichloromethane solvent.

[0032] (4) Stop stirring, centrifuge at 4000rpm for 10 minutes to collect hardened microspheres, wash 3 times with deionized water, and freeze-dry for 24 hours to obtain magnesium oxide and PLGA weig...

Embodiment 2

[0034] (1) 1 gram of lactide-glycolide copolymer (PLGA7525, lactide / glycolide molar ratio 75:25, molecular weight 50,000) was dissolved in 20 milliliters of dichloromethane solution, and magnetically stirred for 12 hours to obtain Solution A.

[0035] (2) Add 20 mg of Span 80 and 0.05 g, 0.1 g or 0.2 g of magnesium carbonate powder to solution A, and homogenize at 12000 rpm for 5 minutes to obtain dispersion B.

[0036] (3) Dissolve 0.2 gram of Tween 60 and 2 gram of polyvinyl alcohol (PVA 1788) in 200 milliliters of deionized water to obtain solution C, and add the dispersion prepared in step (2) dropwise to this solution C under stirring at 300 rpm B. After the dropwise addition, the stirring was continued at room temperature for 8 hours to volatilize the dichloromethane solvent.

[0037] (4) Stop stirring, centrifuge at 4000rpm for 10 minutes to collect hardened and formed microspheres, wash 3 times with deionized water, and freeze-dry for 24 hours to obtain magnesium carb...

Embodiment 3

[0039] (1) 1 gram of lactide-glycolide copolymer (PLGA7525, lactide / glycolide molar ratio 75:25, molecular weight 50,000) was dissolved in 20 milliliters of dichloromethane solution, and magnetically stirred for 12 hours to obtain Solution A.

[0040] (2) Add 20 mg of Span 80, and 0.2 g of magnesium oxide and magnesium carbonate powder to solution A, the mass ratio of magnesium oxide to magnesium carbonate is 3:1, 1:1, 1:3, homogeneous at 12000rpm After 5 minutes, dispersion B was obtained.

[0041] (3) Dissolve 0.2 gram of Tween 60 and 2 gram of polyvinyl alcohol (PVA 1788) in 200 milliliters of deionized water to obtain solution C, and add the dispersion prepared in step (2) dropwise to this solution C under stirring at 300 rpm B. After the dropwise addition, the stirring was continued at room temperature for 8 hours to volatilize the dichloromethane solvent.

[0042] (4) Stop stirring, centrifuge at 4000rpm for 10 minutes to collect hardened microspheres, wash 3 times wit...

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