A magnetic immunochemiluminescent detection kit for apramycin and its application

A chemiluminescence detection and apramycin technology, applied in the field of immunological detection, can solve the problems of high false negative rate and false positive rate, low sensitivity, etc., achieve short detection time, high sensitivity and specificity, and reduce manual operations. effect of error
CN108362896BActive Publication Date: 2020-08-28BEIJING KWINBON BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING KWINBON BIOTECH
Publication Date
2020-08-28

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Abstract

The invention provides a magnetic immunochemiluminescence detection kit for apramycin. The magnetic immunochemiluminescence detection kit comprises an enzyme labeled antigen, an enzyme labeled antigendilution solution, a magnetically labeled antibody, a magnetically labeled antibody dilution solution, apramycin series standard solutions, a chemiluminescent substrate solution A, a chemiluminescentsubstrate solution B, a redissolution solution and a washing solution, wherein the enzyme labeled antigen is horseradish peroxidase-labeled apramycin hapten, and the magnetically labeled antibody isimmunomagnetic bead labeled apramycin monoclonal antibody. The invention further discloses a method for detecting the residual amount of apramycin in milk by using a chemiluminescence detector supporting the kit. According to the present invention, the method has advantages of high sensitivity, high specificity and short detection time in the detection of apramycin.
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Description

technical field

[0001] The invention relates to a chemiluminescence detection kit and a detection method thereof, in particular to a magnetic immunochemiluminescence detection kit for detecting residual apramycin in milk, belonging to the field of immunological detection. Background technique

[0002] Apramycin (Apramycin, Apra), also known as dark mycin, apramycin, abramycin, is a typical representative drug of aminoglycoside antibiotics. Apramycin is a new product developed by Eli Lilly and Company in 1985. It can also be produced in my country at present. It is produced by fermentation of a mutant strain of Streptomyces tenebrarius. Apramycin sulfate is mostly used in feed, which is brown crystalline powder or dull yellow powder with strong polarity, easily soluble in water and slightly soluble in ethanol.

[0003] Improper use of aminoglycoside antibiotics can cause toxic side effects on the kidneys and hearing of animals. Therefore, the maximum residue limits of amino...

Claims

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