Tissue engineering heart valve prosthesis
An artificial heart valve and tissue engineering technology, applied in the medical field, can solve problems such as increasing the risk of death of patients, and achieve the effects of reducing the risk of blood coagulation and inflammation, excellent biocompatibility, and long service life
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Embodiment 1
[0034] The invention relates to a tissue engineering artificial heart valve prosthesis, which is used for heart replacement operation. The prosthesis includes a valve frame 10 and at least two leaflets 20 attached to the valve frame 10 , and the valve frame 10 is fixed on a valve ring 30 . Wherein, the lower edge and the side edge of each leaflet 20 are fixed on the inner side of the leaflet frame 10 . In this embodiment, three leaflets 20 are used, and the structures of the three leaflets 20 are the same or different, and their shapes are completely the same or different. Specifically, the three valve leaflets 20 are evenly arranged on the valve frame 10 and distributed symmetrically in three equal parts.
[0035] Specifically, the valve annulus 30 contains a metal support body inside, and the metal support body is preferably cobalt-chromium alloy, and may also be stainless steel, titanium alloy or nickel-titanium alloy. The outer surface of the valve ring 30 is made of pol...
Embodiment 2
[0045] The basic structure of the prosthesis of Embodiment 2 is consistent with that of Embodiment 1. Wherein the leaflet 20 has a thickness of 350um, a porosity of 30%, and an expanded polytetrafluoroethylene film with an average pore size of 5um, which is prepared by cutting and suturing figure 1 The surgical valve shown, the valve preparation method and structure are the same as those in Example 1.
[0046] The method of using tissue engineering to cultivate fibroblasts on polyester braided leaflets is as follows:
[0047] (1) Wash the skin of the abdomen of human body with a PBS solution containing penicillin and streptomycin to remove subcutaneous fat, then immerse the skin in DMEM culture fluid containing type II collagenase, fetal bovine serum, penicillin and streptomycin, Cultivate overnight at 37°C saturated humidity; the next day pick sweat glands and culture them in sweat gland culture medium at 37°C saturated humidity;
[0048] (2) When the growth of fibroblasts ...
Embodiment 3
[0051] The leaflet 20 is a braided fabric with a thickness of 60 um, a porosity of 10%, and an average fiber spacing (ie, pore size) of 2 um by weaving 10D ultra-high molecular weight polyethylene fibers through plain weave. The braid was prepared into leaflets by the same method as in Example 1 to obtain an ultra-high molecular weight polyethylene valve. In this example, the same tissue engineering method as in Example 1 was adopted, and endothelial tissue was cultured on ultra-high molecular weight polyethylene leaflets through 1-day in vitro culture, with a tissue thickness of 0.1 mm. The endothelial tissue tightly wraps the ultra-high molecular weight polyethylene leaflet, which is firm and not easy to fall off.
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