Application of rodgersia sambucifolia extract in preparation of veterinary drug

An extract and yantuo technology is applied in the field of veterinary medicine preparation in veterinary medicine to achieve the effects of improving immunity, strong activity and wide source of raw materials

Inactive Publication Date: 2018-10-16
YUNNAN AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before this application was filed, there were no relevant reports on the application of the flavonoid crude extract in Yantuo as an additive in the preparation of veterinary drug raw materials

Method used

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  • Application of rodgersia sambucifolia extract in preparation of veterinary drug
  • Application of rodgersia sambucifolia extract in preparation of veterinary drug
  • Application of rodgersia sambucifolia extract in preparation of veterinary drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0035] Transformation experiment of Yantuo flavonoid crude extract on porcine lymphocytes:

[0036] (1) Aseptically collect 20 mL of porcine anterior vena cava blood and put it into an anticoagulant tube containing heparin sodium.

[0037] (2) Dilute the anticoagulant blood and diluent 1:1, mix the diluent and lymphocyte separation solution 1:2, and distribute them into autoclaved centrifuge tubes. Horizontal centrifuge at 1850r / min for 30min Centrifuge.

[0038] (3) Absorb the lymphocyte layer of the white floc in the middle, put it in a clean high-pressure sterilized centrifuge tube, add cleaning solution and mix well, then centrifuge at 1500r / min for 10min; discard the liquid, add 1mL red blood cell lysate and blow evenly, Let it stand for 3 minutes, add cleaning solution to balance, and then centrifuge at 1500r / min for 10 minutes; discard the liquid.

[0039] (4) Complete medium preparation: 1640 medium: fetal bovine serum: double antibody ratio is 89%:10%:1%.

[0040] ...

Embodiment 3

[0049] Improving rat immunity detection experiment:

[0050] On the 0th day of the experiment, 21 experimental rats were divided into Yantuo group, cyclophosphamide control group and blank control group, with 7 rats in each group. After the determination of cells (Gran) and lymphocytes (Lym), except for the blank control group, cyclophosphamide 50 mg / kg was injected once a day for 3 consecutive days. On the 7th day, the samples were collected to measure the WBC, Gran, and Lym indexes of each group respectively. After the sampling, Yantuo group was given 200 mg / kg crude extract of Yantuo flavonoids, and the other groups were given the same amount of normal saline, once a day, for 28 consecutive days. God, the result is like Figure 1-3 As shown, the changes of the influence of Yantuo flavone crude extract on rat WBC, Gran and Lym are shown respectively.

[0051] From the results in the figure, it can be seen that the three immune indexes of the Yantuo group and the cyclophospha...

Embodiment 4

[0053] Acute toxicity test on mice:

[0054] 16 mice were randomly selected and divided into control group, high-dose group, middle-dose group and low-dose group (4 mice in each group, half male and half male). Intragastric administration was carried out according to different dosage groups. The doses of flavonoids were divided into low, medium and high groups as 750, 1500, and 3000 mg / kg body weight respectively. Mice were orally administered at a volume of 20 mL / kg. After administration, observe continuously for 7 days, and observe and record symptoms or death every day.

[0055] Results: There was no death in the mice, the mental state was good, and there were no neurological symptoms such as ataxia or convulsions; the respiratory system had no breathing reduction, difficulty, cessation or inhibition; the defecation was normal, without vomiting or salivation; it moved quickly and felt sensitive to touch. The maximum tolerated dose of the drug is 3000mg.kg-1 body weight. ...

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Abstract

The invention discloses an application of a rodgersia sambucifolia extract in preparation of a veterinary drug. The rodgersia sambucifolia extract is used for preparing a drug additive of the veterinary drug for enhancing the immune function of animal bodies, the rodgersia sambucifolia extract is a crude flavonoid extract extracted from a rodgersia sambucifolia plant, the crude flavonoid extract of rodgersia sambucifolia is applied to the drug additive of the veterinary drug, and the drug concentration in the veterinary drug is 1.25-5 mg/mL. The obtained crude flavonoid extract of rodgersia sambucifolia has good biological activity and can promote transformation of lymphocytes in swine lymphocyte transformation experiments, increase the content of WBCs (white blood cells), Gran (neutrophilicgranulocyte) and Lym (lymphocyte) in serum of rats with low immunogenicity and recover the physical condition of rats with immunosuppression. It is indicated that the crude flavonoid extract of rodgersia sambucifolia has the effect of significantly enhancing the immune function of animal bodies and can be used as a raw material of the veterinary drug for enhancing the immune function of animal bodies.

Description

technical field [0001] The invention belongs to the field of preparation of veterinary medicine in animal medicine, and specifically relates to the application of an extract of rock tuo in the preparation of veterinary medicine. Background technique [0002] With the progress of the times and the continuous development of society, consumers' awareness of food safety and environmental protection is gradually increasing. At present, the problem of veterinary drug residues in the animal food we eat and the pollution of veterinary drugs to the natural environment are becoming more and more important. It is of great significance to find safe and effective drugs to replace antibiotics to prevent and control farmed animal diseases. In recent years, Chinese herbal medicine has attracted public attention in the livestock and poultry breeding industry due to its wide range of effects, low toxicity and side effects, and the characteristics that it is not easy to produce drug resistance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P37/04
CPCA61K36/185A61K2236/333A61K2236/51A61P37/04
Inventor 舒相华宋春莲谢相悦朱福春白华毅李文贵凌万旭罗高吴常月
Owner YUNNAN AGRICULTURAL UNIVERSITY
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