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Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering

A composite hydrogel and gelatin technology, applied in tissue regeneration, medical science, prostheses, etc., can solve the problems of harm to the body, slow bone repair process, low dynamic matching degree, etc., achieve good mechanical properties, promote the formation, The effect of high-intensity biological performance

Active Publication Date: 2018-10-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, hydrogel-based biomaterials still face challenges such as low dynamic matching of mechanical properties, slow and uncontrollable bone repair process, inconsistent degradation rate and repair rate, and degradation products may be harmful to the body.

Method used

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  • Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering
  • Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering
  • Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Preparation of black phosphorus-gelatin composite hydrogel

[0064] 1. A composite hydrogel based on black phosphorus and gelatin, prepared by the following steps:

[0065] (1) Preparation of black phosphorus nanosheets: black phosphorus was ground in N-methylpyrrolidone for 10 min, ultrasonically treated for 10 h at 1000 W, centrifuged at 8000 r / min for 10 min, and the supernatant was taken at 15000 r / min / min centrifugation, the resulting precipitated part is black phosphorus nanosheets, and its diameter is 250-300 nm;

[0066] (2) Preparation of pre-polymerization solution: gelatin with a molecular weight of 10000 was methacrylated and then dissolved in pure water to prepare a 10% methacrylated gelatin solution, and 0.17% (w / v) of DMSO was added Photoinitiator I2959, mix well to get the pre-polymerization solution;

[0067] Among them, the method for methacrylated gelatin is as follows: gelatin with a molecular weight of 10,000 is dissolved in PBS buffer ...

Embodiment 2

[0069] Embodiment 2 performance test

[0070] 1. Carry out performance detection to the composite hydrogel of the present invention

[0071] (1) if figure 1 As shown, the hydrogel prepared by the present invention has a complete shape, is transparent, and has good mechanical properties, and the hydrogel formed at the same time shows different shades of silvery black according to the content of black phosphorus.

[0072] (2) if figure 2 It can be seen from the SEM images that the hydrogel prepared by the present invention has a good pore structure, which can provide a suitable growth space for bone cells, and the bone cells can adhere and grow well, facilitating the growth and reproduction of cells.

[0073] (3) if image 3 As shown, the mechanical performance test proves that under the same conditions, the pressure bearing capacity of the composite hydrogel (BPhydrogel) containing black phosphorus is significantly better than that of the composite hydrogel group (GelMa hyd...

Embodiment 3

[0083] Example 3 Adding Arg-UPEA to black phosphorus-gelatin composite hydrogel

[0084] The other conditions of the preparation method are the same as in Example 1, the only difference is that: in step (3) of the preparation method of the composite hydrogel, 2% (w / v) of arginine-based Unsaturated polyester amide (Arg-UPEA), dissolved and mixed with black phosphorus nanosheets, so that the black phosphorus content is 0.001%.

[0085] The results found that both the composite hydrogel without Arg-UPEA and the composite hydrogel with Arg-UPEA had good biocompatibility and osteogenic ability; compared with the composite hydrogel without Arg-UPEA , the black phosphorus and gelatin-based composite hydrogel added Arg-UPEA further improved the controlled release ability of black phosphorus, the reason may be that the chargeability of Arg-UPEA enhanced the interaction between cells.

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Abstract

The invention discloses a black phosphorus and gelatin based composite hydrogel and an application of the black phosphorus and gelatin based composite hydrogel to bone tissue engineering. The composite hydrogel comprises black phosphorus and gelatin modified by double bonds, wherein the total content of the gelatin modified by double bonds is 5%-99.9%. The biocompatibility of the composite hydrogel is good, the safety is high, the composite hydrogel has excellent mechanical properties and biological performance at the same time, supports the growth and osteogenic differentiation of the cell, and has obvious effects on promoting the generation of new bones and repairing bone defects; a hydrogel matrix having the appropriate performance is specifically constructed by chemically modifying thegelatin, meanwhile, the mineralization speed of the hydrogel is controlled by using the characteristic of the black phosphorus oxidizing in the coexistence of light, oxygen and water and the induction effect of the black phosphorus on the calcium element through the photocontrol, so that the process of repairing the bone is effectively regulated and controlled. The safety of the product is good,the performance is reliable, the operation is convenient, the repeatability is good, and the composite hydrogel is easy to industrialize.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering. More specifically, it relates to a composite hydrogel based on black phosphorus and gelatin, its preparation method and its application in bone tissue engineering. Background technique [0002] The incidence of bone injuries caused by trauma, cancer, and deformation is increasing year by year, and effective treatment strategies are urgently needed. Although autologous bone grafting has always been considered the gold standard for bone repair, this method will affect the original healthy tissue, causing secondary trauma, and at the same time, the source of donors is limited and the cost is high; while allogeneic bone grafting also faces biological Limitations include reduced risk of infection, increased risk of infection, and high costs for sample preparation, handling, and storage. At present, with the development of science and technology and the deepening of basic research, various...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/18A61L27/02A61L27/52A61L27/56
CPCA61L27/025A61L27/18A61L27/222A61L27/52A61L27/56A61L2430/02C08L89/00C08L77/12
Inventor 顾志鹏黄钶晴吴钧
Owner SUN YAT SEN UNIV
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