Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering

A composite hydrogel and gelatin technology, applied in tissue regeneration, medical science, prostheses, etc., can solve the problems of harm to the body, slow bone repair process, low dynamic matching degree, etc., achieve good mechanical properties, promote the formation, The effect of high-intensity biological performance

Active Publication Date: 2018-10-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, hydrogel-based biomaterials still face challenges such as low dynamic matching of mechanical properties, slow and

Method used

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  • Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering
  • Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering
  • Black phosphorus and gelatin based composite hydrogel and application thereof to bone tissue engineering

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0063] Example 1 Preparation of black phosphorus-gelatin composite hydrogel

[0064] 1. A composite hydrogel based on black phosphorus and gelatin, prepared by the following steps:

[0065] (1) Preparation of black phosphorus nanosheets: take black phosphorus and grind it in N-methylpyrrolidone for 10 min, ultrasonically treat it for 10 h, power at 1000 W, centrifuge at 8000 r / min for 10 min, and take the supernatant at 15000 r / min. / min centrifugation, the obtained precipitation part is the black phosphorous nanosheet, the diameter of which is 250~300 nm;

[0066] (2) Preparation of pre-polymerization solution: After methacrylic acid esterification of gelatin with a molecular weight of 10,000, it is dissolved in pure water to prepare a 10% methacrylated gelatin solution, and 0.17% (w / v) dissolved in DMSO is added. Photoinitiator I2959, mix well to obtain pre-polymerization liquid;

[0067] Among them, the method of methacrylic acid esterification of gelatin is: dissolve gelatin with...

Example Embodiment

[0069] Example 2 Performance test

[0070] 1. Perform performance testing on the composite hydrogel of the present invention

[0071] (1) Such as figure 1 As shown, the hydrogel prepared by the present invention has a complete shape, is transparent, and has good mechanical properties. At the same time, the formed hydrogel shows different shades of silver black according to the content of black phosphorus.

[0072] (2) Such as figure 2 It can be seen from the SEM image that the hydrogel prepared by the present invention has a good pore structure and can provide a suitable growth space for bone cells, bone cells can adhere and grow well, and facilitate cell growth and reproduction.

[0073] (3) Such as image 3 As shown, the mechanical performance test proves that under the same conditions, the pressure bearing capacity of the black phosphorus-containing composite hydrogel (BPhydrogel) is significantly better than that of the black phosphorus-free composite hydrogel group (GelMa hydroge...

Example Embodiment

[0083] Example 3 Black phosphorus-gelatin composite hydrogel with Arg-UPEA added

[0084] The other conditions of the preparation method are the same as those in Example 1. The only difference is: in step (3) of the preparation method of the composite hydrogel, 2% (w / v) of arginine-based Unsaturated polyester amide (Arg-UPEA), dissolve and mix with black phosphorus nano-sheets to make the black phosphorus content 0.001%.

[0085] The results show that the composite hydrogel without Arg-UPEA and the composite hydrogel with Arg-UPEA have good biocompatibility and osteogenic ability; compared with the composite hydrogel without Arg-UPEA Arg-UPEA-based composite hydrogel based on black phosphorus and gelatin further improves the controlled release ability of black phosphorus. The reason may be that the electrification of Arg-UPEA enhances the interaction between cells.

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Abstract

The invention discloses a black phosphorus and gelatin based composite hydrogel and an application of the black phosphorus and gelatin based composite hydrogel to bone tissue engineering. The composite hydrogel comprises black phosphorus and gelatin modified by double bonds, wherein the total content of the gelatin modified by double bonds is 5%-99.9%. The biocompatibility of the composite hydrogel is good, the safety is high, the composite hydrogel has excellent mechanical properties and biological performance at the same time, supports the growth and osteogenic differentiation of the cell, and has obvious effects on promoting the generation of new bones and repairing bone defects; a hydrogel matrix having the appropriate performance is specifically constructed by chemically modifying thegelatin, meanwhile, the mineralization speed of the hydrogel is controlled by using the characteristic of the black phosphorus oxidizing in the coexistence of light, oxygen and water and the induction effect of the black phosphorus on the calcium element through the photocontrol, so that the process of repairing the bone is effectively regulated and controlled. The safety of the product is good,the performance is reliable, the operation is convenient, the repeatability is good, and the composite hydrogel is easy to industrialize.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering. More specifically, it relates to a composite hydrogel based on black phosphorus and gelatin, its preparation method and its application in bone tissue engineering. Background technique [0002] The incidence of bone injuries caused by trauma, cancer, and deformation is increasing year by year, and effective treatment strategies are urgently needed. Although autologous bone grafting has always been considered the gold standard for bone repair, this method will affect the original healthy tissue, causing secondary trauma, and at the same time, the source of donors is limited and the cost is high; while allogeneic bone grafting also faces biological Limitations include reduced risk of infection, increased risk of infection, and high costs for sample preparation, handling, and storage. At present, with the development of science and technology and the deepening of basic research, various...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/18A61L27/02A61L27/52A61L27/56
CPCA61L27/025A61L27/18A61L27/222A61L27/52A61L27/56A61L2430/02C08L89/00C08L77/12
Inventor 顾志鹏黄钶晴吴钧
Owner SUN YAT SEN UNIV
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