Process for preparing and separating biobutanol and bioethanol
A technology of bioethanol and biobutanol, which is applied in the field of fermentation, can solve problems such as environmental pollution, achieve good wall-breaking effect, high concentration, and improve fermentation efficiency
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Embodiment 1
[0041] A process for preparing and separating biobutanol and bioethanol, which includes the following steps:
[0042] First crush the corn stalks to within 5 cm, and then place them under a pressure of 1.5 MPa and a residence time of 15 minutes for steam explosion pretreatment, and then blast; the corn stalks after steam explosion treatment are passed through a 50-mesh sieve, and the under-sieve is collected. Then add the undersize material to 2 times the weight of water, heat to 60°C, stir at 100 rpm for 90 min under the insulation condition, then heat to 121°C, hold for 10 min, and cool to room temperature naturally to obtain a culture solution;
[0043] The seed solution of Trichoderma konsiii (density 1×10 8 cfu / mL) and Aspergillus niger seed liquid (density 1×10 8 cfu / mL) mix according to the volume ratio of 2:1, and then inoculate 6-8% of the inoculum into the culture medium, culture for 96 hours, control the stirring speed at 100rpm, culture temperature at 32℃, and control by...
Embodiment 2
[0046] The method for fermentation and production of bio-butanol and bio-ethanol using corn stover as the main raw material includes the following steps:
[0047] First crush the corn stalks to within 5 cm, and then place them under a pressure of 2 MPa and a residence time of 10 min for steam explosion pretreatment, and then blast; the corn stalks after steam explosion treatment are passed through a 50-mesh sieve, and the under-sieve is collected. Add the undersize material to 1.5 times the weight of water, heat to 60°C, stir at 100 rpm for 90 min under the insulation condition, then heat to 121°C, hold for 10 min, and naturally cool to room temperature to obtain a culture solution;
[0048] The seed solution of Trichoderma konsiii (density 1×10 8 cfu / mL) and Aspergillus niger seed liquid (density 1×10 8 cfu / mL) Mix according to the volume ratio of 3:2, and then inoculate 6-8% of the inoculum into the culture solution, culture for 96 hours, control the stirring speed at 100rpm, cult...
Embodiment 3
[0051] The changes of main components of corn stalks caused by blasting are shown in Table 1:
[0052] Table 1
[0053] index
[0054] Conclusion: Blasting damages the cell wall of the straw, and some hemicellulose and cellulose are degraded and dissolved out, which is beneficial to the enzymatic hydrolysis of cellulose by cellulase; the crystallinity and polymerization degree of cellulose decrease during the pretreatment process of blasting, and the hemicellulose passes It is degraded into xylose by self-hydrolysis and can be used as a carbon source for strains.
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