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Bismuth serous membrane test strip and preparation method and application thereof

A serous film and test strip technology, applied in the field of electrochemical detection, can solve the problems of large volume, high cost, secondary pollution, etc., and achieve the effect of simple processing operation, low production cost and high practicability

Active Publication Date: 2018-11-20
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of method can obtain reliable detection results, there are many defects: such as low lead atomization rate, strict requirements on sample processing and working environment, secondary pollution during sample processing and nitrification, need for professionals, measurement accuracy and There are limitations in scope, and it is not suitable for large-scale sample screening, etc.
In addition, the existing commercial blood lead analyzers (such as ESA Company, etc.) mainly use electrochemical analysis methods, such as square wave voltammetry, potential stripping method, etc., which have a small size compared with traditional electrochemical workstations. , less time-consuming, and high detection accuracy, but its application scope is still in hospitals, disease control centers and research centers, while domestic instruments are only suitable for laboratory environments, and there are problems such as backward technology, large size, and inconvenient operation. Suitable for clinical and portable use requirements, it is more difficult to apply to the screening of large batches of samples
At the same time, most of these instruments use mercury film electrodes, which require numerous preparation procedures and high costs. Mercury, as a heavy metal harmful to the human body, needs to be strictly controlled

Method used

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  • Bismuth serous membrane test strip and preparation method and application thereof
  • Bismuth serous membrane test strip and preparation method and application thereof
  • Bismuth serous membrane test strip and preparation method and application thereof

Examples

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preparation example Construction

[0027] The invention provides a kind of preparation method of bismuth serous film test strip, comprises the following steps:

[0028] The first step: the synthesis of the precursor bismuth dodecyl mercaptide

[0029] Dissolve bismuth nitrate pentahydrate in dimethylformamide, heat and stir, and add dodecyl mercaptan drop by drop, and stir at room temperature after the dropwise addition, until a yellow precipitate is formed after fully reacting; after the yellow precipitate is filtered and washed with ethanol The solid phase was collected and dried at a low temperature of 35° C. to 45° C. to obtain a yellow solid, which is the precursor bismuth dodecylmercaptide.

[0030] The second step: preparation of nanobismuth

[0031] Dissolve the bismuth precursor in oleylamine, then add oleic acid and heat and stir at 60°C to 70°C until the precipitate is completely dissolved and turns into a yellow solution; Heating in an oven, then cooling to room temperature; washing the cooled pro...

Embodiment 1

[0043] The present embodiment provides a kind of preparation method of bismuth serous film test strip, comprises the following steps:

[0044] The first step: the synthesis of the precursor bismuth dodecyl mercaptide

[0045] Weigh 2.328g of bismuth nitrate pentahydrate and dissolve it in 10mL of dimethylformamide, add 2.5mL of dodecylmercaptan dropwise while stirring. A yellow precipitate is generated, which is collected after filtration and ethanol washing, and dried in a drying oven at 40°C. The yellow solid obtained after drying is the precursor bismuth dodecyl mercaptide.

[0046] The second step: preparation of nanobismuth

[0047] Accurately weigh 0.500g of the precursor bismuth dodecylmercaptide and dissolve it in 20mL of oleylamine, then add 5mL of oleic acid and stir and heat at 65°C until the precipitate is completely dissolved and turns into a yellow solution, then transfer it to 25mL of hydrothermal reaction Put it in an oven at 120°C and heat it for 1 hour, tak...

Embodiment 2

[0063] The bismuth serous film test strip prepared in embodiment one is used in the detection process of lead content in human blood samples, specifically comprising the following steps:

[0064] S201: Use a pipette to accurately pipette 80 μL of blood sample into a polytetrafluoroethylene beaker, add 2 mL of nitric acid and 3 drops of perchloric acid, heat on a low-temperature electric heating plate until white smoke is emitted, continue heating for 5 min, and then cool down after removal.

[0065] S202: Dissolve the product obtained in S201 in a buffer solution with an acetic acid-sodium acetate buffer solution with a pH value of 5, transfer it to a 20 mL colorimetric tube, and set the volume to the mark.

[0066] S203: Then transfer the prepared blood sample to a 25mL beaker, use the bismuth plasma film as the working electrode, the silver paste as the reference electrode, and the carbon paste as the counter electrode, and test the lead content in the pretreated blood sample...

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Abstract

The invention relates to a bismuth serous membrane test strip and a preparation method and an application thereof. The preparation method of the bismuth serous membrane test strip includes the following steps: performing grinding after bismuth nanoparticles, acetylene black, and an epoxy resin system are mixed to obtain a bismuth sizing agent with epoxy resin as a binder; pouring the bismuth sizing agent on a wire mesh plate, levelling the sizing agent due to the action of gravity, obtaining clear printing pattern, and finally obtaining the bismuth serous membrane test strip. As an environmentfriendly membrane electrode, the bismuth serous membrane test strip can effectively replace a mercury membrane electrode for detection of a lead content in blood samples; and the lead content in pretreated blood samples is tested through employing a bismuth serous membrane as a working electrode, silver paste as a reference electrode, and carbon paste as a counter electrode. The bismuth serous membrane test strip of the invention has the characteristics of high reliability, high selectivity and high practicability, can quickly and accurately detect the concentration of lead ions in the blood,is less in blood using volume, is simple in operation of blood processing, is lower in the production cost, and has a good practical application prospect.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a bismuth serous film test strip, a preparation method and application thereof. Background technique [0002] With the development of industrialization and urbanization, more and more heavy metal ions are released into the environment to produce various pollutions. Lead is one of the most toxic heavy metal pollutants known, which has extremely strong accumulation and irreversible damage in organisms. After lead and its compounds enter the human body, they are distributed to various organs and tissues of the human body along with the blood. Therefore, urine, bones, feces, hair, teeth, and blood all contain lead, and clinical testing can reflect the lead content in the human body to varying degrees. The lead content in the blood is absorbed from the outside on the one hand, and released from the bone tissue on the other hand; therefore, the blood lead level can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/416
CPCG01N27/3272G01N27/416
Inventor 张连明李建平刘军李秋平覃丽孟唐贝兮
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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