Preparation method and application of multilevel orientation porous superfine electric spinning composite fiber membrane

Abstract

The invention relates to a preparation method and application of a multilevel orientation porous superfine electric spinning composite fiber membrane. The method comprises the following steps of firstly adding reducing graphene into a dichloromethane solvent to perform ultrasonic dispersion, then adding reducing graphene dispersion liquid and polylactic acid into an airtight vessel which containsdichloromethane, stirring and performing ultrasonic treatment to prepare a macromolecular solution; in a condition of certain relative humidity, putting the prepared macromolecular solution in an airtight injection syringe, spinning on an electrostatic spinning device to form the multilevel orientation porous superfine electric spinning composite fiber membrane with a stable segment which has a length of 20cm or more. The method can be used for solving the problems that in the prior art, the fiber membrane is poor in electrical performance, the dispersion of graphene is not uniform and the biological activity is low, according to the fiber membrane prepared in the invention, the fiber diameter is relatively fine and can be up to 369-2,350nm, the fiber is in orientation arrangement, the fiber surface has an orientation hole structure, the orientation growth and distribution of cells can be adjusted and controlled, the fiber membrane has relatively high mechanical performance, the fiberbreaking strength is 70-122MPa, and the fiber membrane is mainly applied in biological tissue engineering support materials.

Description

technical field [0001] The invention relates to the technical field of biomedical tissue engineering materials, in particular to a preparation method and application of a multi-level oriented porous ultrafine electrospun composite fiber membrane. Background technique [0002] Polylactic acid (PLA) is a biodegradable biomaterial with good biocompatibility. PLA has attracted much attention in the field of biomedical materials because of its low cost, excellent plastic processability, and stable initial mechanical strength. At present, a large number of basic research and animal experimental research on poly PLA materials have been carried out at home and abroad, and good results have been achieved in bone, blood vessel, nerve, tendon and other tissue engineering scaffolds, and some products have been used clinically. [0003] However, many subtle activities of the human body are related to bioelectricity. External stimuli, heart beating, muscle contraction, and brain thinking ...

AI Technical Summary

Problems solved by technology

However, most of the research in this aspect in the prior art research is about graphene or graphene oxide and polylactic acid electrospun together to prepare tissue engineering scaffold materials. Graphene oxide has the problem of poor conductivity, and the dissolution of graphene in electrospinning solution Poor dispersibility and toxicity, and the current preparation process of graphene is complicated and costly, mainly including micromechanical exfoliation method, chemical vapor deposition method, epitaxial growth method, graphene oxide reduction method, organic synthesis method, solvent exfoliation method and Thermal reduction method, etc.

In contrast, the graphene oxide reduction method has low cost and simple process, and is the method most likely to realize large-scale preparation. Although there are related reports about the preparation of environmentally friendly and non-toxic reduced graphene in the prior art (such as Chinese patent CNIO439909OA), however, there are few reports on doping reduced graphene into oriented porous nanofiber tissue engineering scaffold materials. Electrospun fibers can also disperse reduced graphene evenly in the fibers, which greatly broadens the performance of electrospun fibers.

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