Extraction method of raphanin

Abstract

The invention discloses an extraction method of raphanin. The extraction method comprises the following steps: preparing broccoli powder from stems and/or leaves of broccoli, mixing 2 parts of broccoli powder, 15 to 25 parts of external mustard seed enzyme solution and 10 parts of phosphoric acid buffering solution with the pH of 4.0 to 6.0, forming a primary mixed solution, successively performing the enzymolysis, freeze drying, extraction and suction filtering for the primary mixed solution, obtaining raphanin extraction solution, performing the rotary evaporation for the raphanin extractionsolution to obtain raphanin extract, re-dissolving the extract by using 50 percent acetonitrile, chromatographically collecting by using half prepared liquid phase, and obtaining a raphanin pure product. The proportion of the broccoli powder, the external mustard seed enzyme liquid and the phosphoric acid buffering solution is g: mL: mL. The raphanin is extracted from a broccoli byproduct, so that the byproduct utilization rate can be greatly improved, the additional value of the broccoli can be increased, and the resource waste can be reduced. The process parameters are optimized by virtue of a single-factor experiment and response surface experiment, and a raphanin extraction method which is simple in operation, simple in equipment, rapid, high in efficiency, and high in product puritycan be obtained.

Description

technical field [0001] The invention belongs to the field of comprehensive utilization of horticultural crop product processing by-products, and relates to a method for extracting sulforaphane, an active substance in broccoli processing by-products. Background technique [0002] Sulforaphane (SF), chemical name 1-isothiocyanate-4-methanesulfonyl butane, is a sulfur-containing plant secondary metabolite of glucosinolates in plants under the action of myrosinase . A large number of scientific research reports have confirmed that sulforaphane can effectively prevent the occurrence of various cancers and induce apoptosis of cancer cells, such as gastric cancer cells, colon cancer cells, bladder cancer cells, breast cancer cells, etc. It is the most anti-cancer activity found in plants. strong substance. [0003] In recent years, broccoli seeds are mostly used as raw materials for the extraction of sulforaphane, and a few are obtained from broccoli sprouts, but few studies repo...

AI Technical Summary

Problems solved by technology

In fact, broccoli by-products (stems and leaves) account for more than 30% of the fresh weight of a single broccoli plant, but they are discarded because of their hard texture and unsuitable for consumption, resulting in a great waste. Therefore, research from broccoli by-products It is of great significance to extract sulforaphane from the product

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