Unlock instant, AI-driven research and patent intelligence for your innovation.

Construction of a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold and its surface roughening treatment method

A technology of bone tissue engineering and surface roughening, applied in tissue regeneration, pharmaceutical formulations, prostheses, etc., can solve the problems of unsuitable bone tissue formation, random pore structure of scaffolds, material exchange obstacles, etc., to reduce stress shielding effect, Beneficial for bone fusion and reducing aseptic inflammation

Active Publication Date: 2020-11-27
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although traditional scaffold manufacturing technologies such as solvent casting method / particle filter method, gas foaming method, phase separation method, emulsion freeze-drying method, and freeze-drying method can meet some conditions for ideal scaffold construction, they have inherent defects that cannot be remedied: (1) Lack of control over fine pore size, structure, and spatial distribution, and the pore structure of the prepared scaffold is random, which is not suitable for bone tissue formation; (2) Poor reproducibility; organic solvents used in the preparation process are toxic to cells (3) The inward growth ability of bone tissue is not good, and the cells cannot migrate to the deep part of the scaffold, but only adhere and colonize around the scaffold, which will hinder the exchange of oxygen and nutrients in the later stage of bone tissue formation , and after the formation of mineralized tissue on the surface of the stent, the hindering effect will be more obvious; (4) What is required for the construction of individualized stents for patients is also beyond the reach of traditional methods
[0007] The inventor's research group conducted surface modification on the PCL-TCP bone tissue engineering scaffold through organic solvent etching (the volume ratio of acetone and dichloromethane is 9:1), and found that good surface roughening results could not be obtained.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction of a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold and its surface roughening treatment method
  • Construction of a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold and its surface roughening treatment method
  • Construction of a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold and its surface roughening treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. PCL and TCP materials are uniformly mixed and granulated

[0033] PCL (Sigma, MW 65000) particles and nano-TCP particles (Sigma) were mixed according to the mass ratio of 8:2, uniformly mixed by a torque rheometer (Rheomix 600, Haake, Germany) and drawn, and then granulated Machine cutting into granular raw materials for 3D printing.

[0034] 2. Preparation of PCL-TCP bone tissue engineering scaffold by 3D printing technology

[0035] According to the PCL-TCP fiber diameter of 0.5mm, 0 / 45 / 90 / 135° layer and layer arrangement, the aperture is 500 μm, and the layer height is 0.3mm to build the model. After the 3D printing software automatically generates the nozzle walking path, set the temperature of the material chamber 90°C, nozzle temperature 80°C. Run according to the program to obtain the PCL-TCP bone tissue engineering scaffold.

[0036] 3. The organic solvent etching method completes the surface roughening treatment of the stent

[0037] At room temperature,...

Embodiment 2

[0042] 1. PCL and TCP materials are uniformly mixed and granulated

[0043] PCL (Sigma, MW 65000) particles and nano-TCP particles (Sigma) were mixed according to the mass ratio of 8:2, and were evenly mixed by a torque rheometer (Rheomix 600, Haake, Germany) before drawing, and then granulated Machine cutting into granular raw materials for 3D printing.

[0044] 2. Preparation of PCL-TCP bone tissue engineering scaffold by 3D printing technology

[0045] According to the PCL-TCP fiber diameter of 0.4mm, 0 / 60 / 120° layer and layer arrangement, hole diameter of 300 μm, and layer height of 0.2mm, the model is established. After the 3D printing software automatically generates the nozzle walking path, set the temperature of the material chamber to 90°C , nozzle temperature 80 ℃. Run according to the program to obtain the PCL-TCP bone tissue engineering scaffold.

[0046] 3. The organic solvent etching method completes the surface roughening treatment of the stent

[0047] At r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
heightaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of bone tissue engineering, in particular to a construction and surface roughening treatment method of a polycaprolactone-tricalcium phosphate bone tissueengineering scaffold. The method combines advantages of polycaprolactone and tricalcium phosphate through a 3D printing technology, and a constructed PCL-TCP 3D printing scaffold improves the surfaceroughness level through organic solvent etching to further enhance the adhesion and colonizing capacity to the scaffold by bone cell. The method provides a certain theoretical basis for the application of bone graft substitutes.

Description

technical field [0001] The invention relates to the technical field of bone tissue engineering, in particular to the construction of a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold and its surface roughening treatment method. Background technique [0002] Whether in Asia or Europe and the United States, the incidence of fractures, bone tumors and other diseases and the expenditure on bone transplantation continue to rise. The clinical treatment of bone defects caused by trauma, deformity, degeneration, and tumors is inseparable from bone tissue reconstruction. When reconstructing bone tissue structure, suitable filling materials are needed to replace the original missing bone. As the gold standard for bone defect repair, autologous bone has ideal properties of induction, conduction, and osteogenesis. However, its source is limited, and complications such as donor site infection and chronic pain limit its development. Allograft bone includes allogen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/58C08J7/12C08L67/04
CPCA61L27/446A61L27/58A61L2300/10A61L2300/412A61L2300/604A61L2300/622A61L2400/18A61L2430/02C08J7/12C08J2367/04C08L67/04
Inventor 陈雄生周盛源苑博王智巍唐一钒
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV