Production method of bilirubin adsorbent

A production method and technology of bilirubin, which are applied in the field of biomedicine, can solve the problems of high price and restrictions on the application of such products, and achieve the effects of good mechanical strength, safe and effective treatment, and strong adsorption capacity

Inactive Publication Date: 2019-01-29
山阳县恒桓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] At present, there is no special safe and effective bilirubin adsorbent product in our country, and Japanese products are expensive, which greatly limits the application of such products in our country

Method used

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  • Production method of bilirubin adsorbent
  • Production method of bilirubin adsorbent
  • Production method of bilirubin adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, polyvinyl alcohol microspheres are the preparation of the adsorbent of carrier

[0032] a. Preparation of polyvinyl alcohol microspheres

[0033] Add 24g of vinyl acetate, 6g of triallyl isocyanurate, 15ml of ethyl acetate, 15ml of n-heptane and 1.6g of polyvinyl acetate (polymerization degree 500) into a 500ml three-necked flask, and the system is heated to 40°C. Mechanical stirring, add 0.3g initiator azobisisobutyronitrile after the polyvinyl acetate dissolves completely, add 300ml aqueous solution (by weight, containing 1.5% polyvinyl alcohol and 3% chlorine Sodium chloride), adjust the stirring speed to make the droplets uniformly dispersed and suitable in size, slowly raise the temperature to 65°C, keep the temperature and stirring speed for polymerization for 3 hours, then raise the temperature to 75°C for another 3 hours to obtain a white granular copolymer. Filter, wash with hot water, extract with acetone in a Soxhlet extractor for 12 hours, and...

Embodiment 4

[0038] Example 4. Fixation of 1,6-hexanediamine functional group: Take 1ml of the activated microspheres obtained in the step of Example 1b, add 0.75ml 2mol / L NaOH, 5ml 1,6-hexanediamine, shake overnight at 75°C , and then fully washed with distilled water to obtain a bilirubin adsorbent for future use.

Embodiment 5

[0039] Embodiment 5, adsorbent in vitro static adsorption experiment: measure 0.5ml respectively by embodiment 2, embodiment 3, the adsorbent prepared in embodiment 4, place in the polypropylene plastic tube of 10.0ml, add appropriate amount of normal saline Equilibrate for 3 hours, absorb free physiological saline, add 5.0ml of bilirubin simulated serum solution with a concentration of 342μmol / L (a certain amount of bilirubin is dissolved in PBS buffer, which contains 20g / L albumin), 37°C Shake at constant temperature for 3 hours, let it stand for a while, absorb the supernatant to measure the concentration of bilirubin, and set a blank control, the adsorption rate is calculated by the following formula:

[0040] AP=([C]B-[C]A) / [C]B×100%

[0041] Where AP is the adsorption rate (%), [C]B and [C]A are the concentrations before and after adsorption (mg / dl).

[0042] By embodiment 2, embodiment 3, the adsorption rate of the adsorbent that embodiment 4 makes is respectively 93%,...

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Abstract

The invention provides a production method of a bilirubin adsorbent, in which a polyvinyl alcohol microsphere having excellent biocompatibility serves as a carrier and is activated by epoxy chloropropane; then an amination reagent is immobilized as a functional group to form the bilirubin adsorbent. The bilirubin adsorbent carries a large number of positive charges on the surface, which is beneficial to generate adsorption effect with the bilirubin that carries negative charges on the surface. The bilirubin adsorbent has good biocompatibility and adsorption effect, is low in production cost, has simple synthetic process, is large in adsorption quantity and is high in adsorption speed. The bilirubin adsorbent can be used for treating hyperbilirubinemia through in-vitro whole blood perfusion.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. The invention relates to a preparation method of a bilirubin adsorbent. Background technique [0002] Bilirubin (Bilirubin, BR) is a kind of bile pigment, a metabolite of aging red blood cells. In the body, bilirubin is generally combined with glucuronic acid to form a water-soluble complex, which is transported by the blood to the liver for metabolism, and passes through the biliary tract. excretion. If there is too much bilirubin in the body, or liver damage, bilirubin blockage and other reasons can cause a large amount of bilirubin to accumulate in the body, resulting in hyperbilirubinemia, the bilirubin level in severe patients can reach 700 μmol / L . Free bilirubin is an endogenous toxin that can cause abnormal functions of organs such as the liver and biliary tract. Due to its fat solubility, it can easily pass through various biological membranes and deposit in tissues, causing yello...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01D15/08B01J20/30C08F8/32C08F8/00C08F8/12C08F218/08
CPCB01D15/08B01J20/264C08F8/32C08F8/00C08F8/12C08F218/08
Inventor 鲁保存
Owner 山阳县恒桓生物科技有限公司
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