Fluorescent microspheres with carboxyl group-enriched surface and synthesis method of fluorescent microspheres

Abstract

The invention relates to fluorescent microspheres with a carboxyl group-rich surfaces and a synthesis method of the fluorescent microspheres. The surface of the microspheres is rich in carboxyl groups, and fluorescent molecule hybridization is performed due to rare earth coordination on the surface; and the microspheres have a particle size of 0.1-2 [mu]m. The preparation method has good controllability and high reproducibility, and the prepared microspheres have uniform particle size, controllable size, high fluorescence efficiency and stable dispersion in a large range of pH, can be stably dispersed in water, ethanol, acetone, acetonitrile and other solvents, and have non-toxic required raw materials, environmental protection, low price, simple operation and low equipment requirements; the microspheres can be more widely applied to chemistry, biology, material science and other fields, and since the prepared microspheres have higher density of surface carboxyl groups than common carboxylated microspheres, proteins, DNA or other molecules with amino terminals can be immobilized on the microspheres; and it is expected that the microspheres have broad application prospects in the field of biomedicine, especially in fluorescence imaging, cancer radiation therapy, fluorescent labeling, fluorescence analysis and other aspects.

Description

technical field [0001] The invention relates to a fluorescent microsphere rich in carboxyl groups on the surface and a synthesis method thereof, and relates to a polymer material and the technical field of synthesis thereof. Background technique [0002] Fluorescent microspheres generally refer to microspheres marked with fluorescent substances on the surface of the microspheres or microspheres containing fluorescent substances in the internal structure of the microspheres, which can excite fluorescence when stimulated by external energy. Balls can be divided into two categories: one is inorganic / organic fluorescent microspheres, and the other is organic / organic microspheres; among them, organic / organic microspheres can be conveniently modified with various functional groups on their surface , Different comonomers and polymerization processes can be selected for the design of polymer microspheres, and because the density of the microspheres is similar to that of water, the b...

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Problems solved by technology

[0003] Commonly used methods for preparing fluorescent microspheres include physical adsorption, self-assembly, embedding, chemical bond summation, and copolymerization. However, in the specific synthesis steps, the existing synthesis methods have poor stability and the prepared The particle size of microspheres is relatively uncontrollable, and the synthesis method is complicated, the price is high, and further improvement is needed

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