Bacillus subtilis BYS2 with high-efficiency antibacterial activity

A Bacillus subtilis, high-efficiency technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of endangering human and livestock and poultry health, increasing the level of bacterial resistance, and degrading the quality of livestock and poultry products, etc. It has the effect of maintaining intestinal homeostasis and no toxic and side effects

Active Publication Date: 2019-02-15
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics play a key role in the prevention and treatment of bacterial diseases, but with the abuse of antibiotics, it will lead to a series of disadvantages such as the decline in the quality of livestock and poultry products due to drug residu

Method used

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  • Bacillus subtilis BYS2 with high-efficiency antibacterial activity
  • Bacillus subtilis BYS2 with high-efficiency antibacterial activity
  • Bacillus subtilis BYS2 with high-efficiency antibacterial activity

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] 1. Obtaining Taishan Bacillus subtilis

[0032] (1) Medium components

[0033] ① Nutrient broth medium (g / L): peptone 10.0, beef extract powder 3.0, sodium chloride 5.0, pH 7.2.

[0034] ② Nutrient agar medium (g / L): peptone 10.0, beef extract powder 3.0, sodium chloride 5.0, agar 15.0, pH 7.3.

[0035] ③ Eosin methylene blue medium (g / L): peptone 10.0, lactose 10.0, dipotassium hydrogen phosphate 2.0, agar 15.0, eosin 0.4, methylene blue 0.065, pH 7.0.

[0036] (2) Isolation of bacterial strains: Take 100 g of soil below 10 cm of the soil surface in four different locations of Mount Tai, put the collected soil into a sterile sterilization bag and bring it back to the laboratory for isolation. Weigh 10 g of soil in each soil layer and put them into conical flasks containing 100 mL of sterile deionized water, shake for 15 min, and bathe in 80°C water for 1 h, then add 7 g of NaCl, and bathe in 80°C water for 1 h. Stand still for 10 minutes, absorb 0.5mL of the superna...

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Abstract

The invention relates to bacillus subtilis BYS2 with high-efficiency antibacterial activity, which has a preservation number of CGMCC NO.14220. The bacillus subtilis BYS2 has the advantages of high temperature resistance, acid resistance and cholate resistance; the bacillus subtilis BYS2 can adapt to the gastrointestinal environment and has significant inhibitory effects on escherichia coli, staphylococcus aureus and clostridium perfringens; the bacillus subtilis BYS2, as a feed additive, has the advantages that the ability (p is smaller than 0.05) of animal body to resist infection of pathogenic microorganisms can be improved, the growth performance of broilers is significantly promoted and breeding benefits are improved; meanwhile, the bacillus subtilis BYS2 has the characteristics of safety, high efficiency and probiotic function as well as no toxic or side effects on animal bodies.

Description

field of invention [0001] The invention relates to a strain of Bacillus subtilis BYS2 with high-efficiency antibacterial performance. Background of the invention [0002] Bacillus subtilis can secrete substances such as bacteriocins or antimicrobial peptides to inhibit the growth of intestinal pathogenic bacteria such as Escherichia coli, and Bacillus subtilis can decompose starch into simple sugars, synthesize lactic acid through intestinal flora metabolism, and reduce intestinal pH value, and enhance the inhibitory effect on intestinal pathogenic bacteria. Bacillus subtilis also has the function of biological oxygen capture, forming an anaerobic environment in the intestinal tract, which is conducive to the growth of anaerobic beneficial bacteria (lactic acid bacteria, etc.), inhibits the reproduction of pathogenic bacteria, and helps to improve the intestinal micro-ecological environment. Bacillus subtilis can produce amylase, protease and other digestive enzymes, improv...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/125
CPCA23K10/18C12N1/205C12R2001/125
Inventor 柴同杰郝光恩郭梦娇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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