A pretreatment method and quantitative detection method of endogenous phosphate sugar compounds in plant samples

A phosphate sugar, endogenous technology, applied in the field of analytical chemistry, can solve the problems of detection interference, mass spectrometer damage, low sensitivity, etc., achieve calibration operation errors and instrument errors, enhance stability and accuracy, and improve detection sensitivity Effect
CN109358134BActive Publication Date: 2021-06-01WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2021-06-01

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Abstract

The invention discloses a method for analyzing various phosphate sugar compounds including endogenous trehalose-6-phosphate in plant samples. In this method, endogenous phosphate sugars are first extracted with a solvent, and then the hydrophobic impurities in the extract are adsorbed and removed by a reversed-phase SPE column, and then the phosphate group shared by the diazo group in the derivatization reagent and the phosphate sugar compound is used The group derivatization reaction, combined with liquid chromatography-mass spectrometry, realized the separation and detection of endogenous sugar phosphate isomers in plant samples. The method is simple, accurate, and high-throughput. It can not only realize the baseline separation of sugar phosphate isomers on a hydrophilic chromatographic column, but also ensure high detection sensitivity. Finally, it can achieve endogenous Separation and detection of sexual phosphate sugars.
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Description

technical field

[0001] The invention relates to a method for pretreatment and quantitative analysis of endogenous sugar phosphate compounds in plant samples, belonging to the field of analytical chemistry. Background technique

[0002] Phosphosaccharides are important signaling molecules in plants and play important roles in many physiological processes including seed development, juvenile-adult transition, floral transition, bud branching, senescence and embryonic maturation. However, the mechanism of action of phosphate sugars in plants is still unknown. Therefore, the establishment of accurate and highly sensitive analytical methods is the key to exploring the role of phosphate sugar compounds in plants.

[0003] So far, many analytical methods have been developed for the analysis and detection of phosphate sugar compounds in plants. Among these methods, the LC-MS method has become the mainstream method for the analysis of phosphate sugars due to its high sensitivity an...

Claims

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