Fungicides Used in Cage Culture of Wide-bodied Leech
A wide-bodied golden leech and net cage culture technology, applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of no clear effect on diseases, complex drug components, etc., achieve large specific surface area, strong Bactericidal and antibacterial, increase the load effect
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Embodiment 1
[0035] The fungicide used in cage culture wide body leeches, the preparation method is as follows:
[0036](1) Titanium dioxide nanotube arrays were prepared by anodic oxidation, and the experimental conditions were: electrolyte composition: 1.5ml deionized water and 0.28g ammonium fluoride were added to 100ml ethylene glycol; oxidation parameters: DC voltage 40V, temperature 40°C Electrolyze for 3 hours under the same conditions; after the electrolysis is completed, wash, dry, and roast in a muffle furnace at 400°C for 2 hours;
[0037] (2) Carry out metal deposition on the surface of titanium dioxide by impregnation reduction method: vacuum impregnate the titanium dioxide obtained in step (1) with a silver nitrate solution with a metal deposition amount of 1% for 2 hours, then wash, dry, and roast in a muffle furnace at 400° C. for 2 hours;
[0038] (3) carry out surface polarization to the silver supported titania of step (2) gained:
[0039] When titanium dioxide is wette...
Embodiment 2
[0047] The fungicide used in cage culture wide body leeches, the preparation method is as follows:
[0048] (1) Titanium dioxide nanotube arrays were prepared by anodic oxidation, and the experimental conditions were: electrolyte composition: 1.5ml deionized water and 0.28g ammonium fluoride were added to 100ml ethylene glycol; oxidation parameters: DC voltage 40V, temperature 40°C Electrolyze for 3 hours under the same conditions; after the electrolysis is completed, wash, dry, and roast in a muffle furnace at 400°C for 2 hours;
[0049] (2) Metal deposition on the surface of titanium dioxide by photoreduction method: 0.5% silver nitrate, 0.5% copper sulfate, and 0.5% zinc sulfate solution are used as precursors for metal deposition, and 0.12% by mass of titanium dioxide is added to the mixed solution succinic acid and 0.02% 3-penten-2-alcohol, after ultrasonic reaction for 15min, carry out ultraviolet light (irradiance is 10mW / cm 2 ) irradiation for 2 hours; after the react...
Embodiment 3
[0065] Experiments were carried out on the cultivation of wide-bodied leeches in cages, and the experiment was set as 6 treatment groups, which were respectively control group, embodiment 1-2, and comparative examples 1-3; , Utilize the sieving method to put the similar ones in the same net cage to raise, divide into 6 net cages and put them in the pond, 100 wide-body golden leeches in each net cage; do not put the present invention in the control group pond Bactericides, the fungicides prepared by corresponding embodiments or comparative examples were put into the ponds of embodiments 1-2 and comparative examples 1-3 respectively, and the dosage was 10g / L; The growth situation and immune situation of leeches are as shown in Table 1; the mortality rate and immune protection rate of each group are calculated, and the immune protection rate is calculated according to the following formula:
[0066] Immune protection rate=[1-(immune group mortality / control group mortality)]*100%;...
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