Method for removing heavy metals in pig manure by using biochar enhanced indigenous ferrous oxide bacteria

A ferrous oxide and biochar technology, applied in the field of environmental engineering, can solve the problems of slow growth rate of ferrous oxide bacteria, loss of nutrients, long growth cycle, etc., to accelerate electron transfer, promote growth and reproduction, and increase growth. Effect

Inactive Publication Date: 2019-03-12
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth rate of ferrous oxide bacteria is generally slow and the growth cycle is long, and the sludge bioleaching process will cause a large amount of nutrients such as nitrogen and phosphorus to be lost, and the loss rates of total nitrogen (TN) and total phosphorus (TP) can respectively reach 20-33% and 17-68%
[0005] Aiming at the incomplete removal of heavy metals in livestock and poultry feces solidification technology, it is easy to cause secondary pollution, and the conventional bioleaching technology has a long cycle, low removal efficiency, and serious nutrient loss. This project is specially launched to strengthen the ferrous oxide bacteria with biochar. Research on the separation of heavy metals from livestock and poultry manure by bioleaching, in order to provide technical support and engineering practice reference for the removal of heavy metals in livestock and poultry waste in my country, to ensure the harmless resource utilization of livestock and poultry waste in the process of resource utilization, and to provide food production, Provide a strong guarantee for food safety and human health

Method used

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  • Method for removing heavy metals in pig manure by using biochar enhanced indigenous ferrous oxide bacteria
  • Method for removing heavy metals in pig manure by using biochar enhanced indigenous ferrous oxide bacteria
  • Method for removing heavy metals in pig manure by using biochar enhanced indigenous ferrous oxide bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Sampling

[0036] The test pig manure was taken from the dry clean manure of a farm in Xiqing District, Tianjin. The basic characteristics of the pig manure are shown in Table 1.

[0037] Table 1 Basic characteristics of tested pig manure

[0038]

[0039] (2) Preparation of biochar

[0040] Cut the straw into small sections of 3-5 cm and put them into a sealed reactor; after sealing, put the reactor into a muffle furnace for anoxic calcination. The calcination temperature is 500°C, the calcination time is 2h, and the heating rate is 10°C / min; after the calcination is completed, the biochar is ground with an agate mortar and passed through a 100-mesh standard sieve.

[0041] (3) Preparation of indigenous ferrous oxidizing bacteria inoculum

[0042] 9K medium preparation: (NH 4 ) 2 SO 4 , 3g L -1 ; KCl, 0.1g L -1 ; K 2 HPO 4 , 0.5g L -1 ; MgSO 4 ·7H 2 O, 0.5g L -1 ; Ca(NO 3 ) 2 , 0.01g L -1 ; FeSO 4 ·7H 2 O, 44.72g L -1 ; Distilled water 1L. Pre-cultivation: Dilute fresh pig ...

Embodiment 2

[0052] (1) Sampling

[0053] The test pig manure was taken from the dry clean manure of a farm in Wuqing District, Tianjin. The basic characteristics of the pig manure are shown in Table 4.

[0054] Table 4 Basic characteristics of tested pig manure

[0055]

[0056] (2) Preparation of biochar, same as in Example 1

[0057] (3) The preparation of indigenous ferrous oxidizing bacteria inoculum is the same as in Example 1

[0058] (4) Bio-leaching removal reaction of heavy metals in pig manure

[0059] Dilute the original pig manure to a solid content of 2%, add 10g / L biochar and 20g / L ferrous sulfate to the pig manure, inoculate 10% (volume ratio) indigenous ferrous oxide bacteria inoculum, at 28°C Aeration and stirring reaction for 8-10 days. Set up a control treatment without biochar. After the leaching reaction is completed, the content of heavy metals (Cu, Zn, Mn, Cd) in the pig manure leachate is determined.

[0060] (5) Result analysis

[0061] After the treated pig manure was cent...

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Abstract

The invention discloses a method for removing heavy metals in pig manure by using biochar enhanced indigenous ferrous oxide bacteria; the method can be used for removing heavy metals in the pig manureand other livestock and poultry manure by using the biochar enhanced indigenous ferrous oxide bacteria. Biochar is prepared by carrying out anoxic calcination on wheat straw at 500 DEG C. The methodcomprises the steps of adding the pig manure to be treated into a bioreactor, respectively inoculating 10-15% of indigenous ferrous oxide bacteria and 5-10g / L of biochar, and carrying out aeration treatment for 4-7 days at 30+ / 2 DEG C; pumping out the treated pig manure, dehydrating the pig manure, adjusting the pH of a liquid phase part to precipitate the heavy metals, and neutralizing a solid phase part so as to realize the agricultural utilization of the pig manure. The removal rate of the heavy metals in the pig manure reaches up to 90-98%, and the nutrient loss in the pig manure is reduced by 10% or above. The method is simple and convenient to operate, economically feasible and safe, and is an efficient method for removing the heavy metals in the pig manure.

Description

1. Technical Field [0001] The invention relates to a method for removing heavy metals from pig manure by using biochar to strengthen indigenous ferrous oxidizing bacteria. It uses the redox activity and adsorbability of biochar to promote the growth of ferrous oxidizing bacteria to remove heavy metals in livestock and poultry manure while reducing nutrients The technology of material loss belongs to the field of environmental engineering technology. 2. Background technology [0002] In recent years, with the rapid development of large-scale livestock and poultry breeding, a large amount of livestock manure is produced every year. According to statistics, the output of livestock manure in 2010 was 243 million tons. Since livestock manure is rich in nutrients such as nitrogen, phosphorus, organic matter, etc., it is widely used as agricultural soil fertilizer. However, in the production process of livestock and poultry, in order to promote animal growth, increase feed utilization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01C02F101/20C02F103/20
CPCC12N1/20C02F11/02C02F2101/20C02F2103/20
Inventor 魏孝承刘东方黄文力
Owner NANKAI UNIV
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