Method for removing heavy metals in pig manure by using biochar enhanced indigenous ferrous oxide bacteria
A ferrous oxide and biochar technology, applied in the field of environmental engineering, can solve the problems of slow growth rate of ferrous oxide bacteria, loss of nutrients, long growth cycle, etc., to accelerate electron transfer, promote growth and reproduction, and increase growth. Effect
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Embodiment 1
[0035] (1) Sampling
[0036] The test pig manure was taken from the dry clean manure of a farm in Xiqing District, Tianjin. The basic characteristics of the pig manure are shown in Table 1.
[0037] Table 1 Basic characteristics of tested pig manure
[0038]
[0039] (2) Preparation of biochar
[0040] Cut the straw into small sections of 3-5 cm and put them into a sealed reactor; after sealing, put the reactor into a muffle furnace for anoxic calcination. The calcination temperature is 500°C, the calcination time is 2h, and the heating rate is 10°C / min; after the calcination is completed, the biochar is ground with an agate mortar and passed through a 100-mesh standard sieve.
[0041] (3) Preparation of indigenous ferrous oxidizing bacteria inoculum
[0042] 9K medium preparation: (NH 4 ) 2 SO 4 , 3g L -1 ; KCl, 0.1g L -1 ; K 2 HPO 4 , 0.5g L -1 ; MgSO 4 ·7H 2 O, 0.5g L -1 ; Ca(NO 3 ) 2 , 0.01g L -1 ; FeSO 4 ·7H 2 O, 44.72g L -1 ; Distilled water 1L. Pre-cultivation: Dilute fresh pig ...
Embodiment 2
[0052] (1) Sampling
[0053] The test pig manure was taken from the dry clean manure of a farm in Wuqing District, Tianjin. The basic characteristics of the pig manure are shown in Table 4.
[0054] Table 4 Basic characteristics of tested pig manure
[0055]
[0056] (2) Preparation of biochar, same as in Example 1
[0057] (3) The preparation of indigenous ferrous oxidizing bacteria inoculum is the same as in Example 1
[0058] (4) Bio-leaching removal reaction of heavy metals in pig manure
[0059] Dilute the original pig manure to a solid content of 2%, add 10g / L biochar and 20g / L ferrous sulfate to the pig manure, inoculate 10% (volume ratio) indigenous ferrous oxide bacteria inoculum, at 28°C Aeration and stirring reaction for 8-10 days. Set up a control treatment without biochar. After the leaching reaction is completed, the content of heavy metals (Cu, Zn, Mn, Cd) in the pig manure leachate is determined.
[0060] (5) Result analysis
[0061] After the treated pig manure was cent...
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