Pediococcus pentosaceus and application thereof
A technology of Pediococcus pentosaceus and strains, applied in the field of microorganisms, can solve problems such as mammalian constipation, achieve high safety, high industrial application value, and the effect of relieving constipation
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Embodiment 1
[0021] Embodiment 1 Pediococcus pentosacea ( Pediococcus pentosaceus ) Isolation and identification of B49
[0022] 1. Strain isolation
[0023] Colostrum was obtained from healthy women in Fujian, China, who gave birth to a baby no more than 14 days old. The fresh colostrum was placed in a clean bench and diluted with sterile PBS solution. The undiluted milk and the diluted milk were distributed to MRS culture respectively. Base. Cultivate at a constant temperature at 37°C until colonies appear. Use an inoculation needle to pick out well-growing bacteria on the edge and inoculate them on a new MRS plate medium. After the newly inoculated bacteria grow into colonies, pick the endogenous bacteria on the edge. Bacteria were streak cultured and purified repeatedly until a single colony was obtained, which was named strain B49.
[0024] The formula of MRS liquid medium is: tryptone 10.0 g / L, beef powder 5.0 g / L, yeast powder 4.0 g / L, glucose 20.0 g / L, Tween 80 1.0 mL / L, K 2 H...
Embodiment 2
[0029] Embodiment 2 Pediococcus pentosacea ( Pediococcus pentosaceus ) Utilization of B49 to carbohydrates
[0030] Application of API50CHL kit (Biomerieux, France) to detect the utilization of sugars by the bacterial strain B49 isolated in Example 1, the results show that: Pediococcus pentosaceae B49 of the present invention can utilize L-arabinose, D-xylose, ribose, D - Glucose, D-mannose, D-fructose, D-galactose, D-lactose, D-cellobiose, maltose, trehalose, D-gentiobiose.
Embodiment 3
[0031] Embodiment 3 Pediococcus pentosacea ( Pediococcus pentosaceus ) Enzyme activity of B49
[0032] Application of APIZYM kit (Biomerieux, France) to detect the enzymatic activity of the isolated bacterial strain B49 in Example 1, the results show that: Pediococcus pentosaceae B49 of the present invention can secrete leucine aryl amidase, valine aryl amidase, Acid phosphatase, naphthol-As-diphosphate hydrolase, β-glucosidase, N-acetyl-β-glucosaminidase, cystine aryl amidase.
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