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A technology of Pediococcus pentosaceus and strains, applied in the field of microorganisms, can solve problems such as mammalian constipation, achieve high safety, high industrial application value, and the effect of relieving constipation
Active Publication Date: 2019-03-22
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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[0005] The purpose of the present invention is to provide a strain of Pediococcus pentosaceae and its application, and develop a bacterial strain with strong laxative effect and strong inhibitory ability to Staphylococcus aureus a
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[0021] Example 1 Pediococcus pentosaceus ( Pediococcus pentosaceus ) Isolation and identification of B49
[0022] 1. Strain isolation
[0023] Obtain colostrum from healthy women in Fujian, China who gave birth to a baby not more than 14 days old. Place the obtained fresh colostrum on a clean bench and dilute it with a sterile PBS solution. Distribute the undiluted milk and the diluted milk to the MRS culture. 基中. Incubate at 37°C at a constant temperature until colonies appear. Use an inoculating needle to pick out the well-growing bacteria on the edge and inoculate it on a new MRS plate medium. After the newly inoculated bacteria grow into bacteria, pick the edge of the bacteria. The bacteria were cultured in streaks and purified repeatedly until a single colony was obtained, which was named as strain B49.
[0024] The formula of MRS liquid medium is: tryptone 10.0 g / L, beef powder 5.0 g / L, yeast powder 4.0 g / L, glucose 20.0 g / L, Tween 80 1.0 mL / L, K 2 HPO 4 • 3H 2 O 1.5 g / L, anh...
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[0029] Example 2 Pediococcus pentosaceus ( Pediococcus pentosaceus ) Utilization of B49 to sugar
[0030] The API50CHL kit (Biomerieux, France) was used to test the sugar utilization of the strain B49 isolated in Example 1. The results showed that Pediococcus pentosaceus B49 of the present invention can utilize L-arabinose, D-xylose, ribose, and D -Glucose, D-mannose, D-fructose, D-galactose, D-lactose, D-cellobiose, maltose, trehalose, D-gentiobiose.
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[0031] Example 3 Pediococcus pentosaceus ( Pediococcus pentosaceus ) Enzyme activity of B49
[0032] The APIZYM kit (Biomerieux, France) was used to detect the enzyme activity of the strain B49 isolated in Example 1. The results showed that Pediococcus pentosaceus B49 can secrete leucine aryl amidase, valine aryl amidase, Acid phosphatase, naphthol-As-diphosphate hydrolase, β-glucosidase, N-acetyl-β-glucosaminidase, cystine aryl amidase.
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Abstract
The invention discloses pediococcus pentosaceus. The preservation name of a strain is pediococcus pentosaceus B49 and is preserved in China General Microbiological Culture Collection Center, CGMCC, and the preservation number is CGMCC No. 15957. The invention further discloses an application of the pediococcus pentosaceus in preparing bowel relaxing drugs, health foods or common foods, an application of the pediococcus pentosaceus in preparing drugs for inhibiting staphylococcus aureus, health foods or common foods and an application of the pediococcus pentosaceus in preparing drugs for inhibiting vibrio vulnificus, health foods or common foods. The safety of the pediococcus pentosaceus B49 disclosed by the invention is high; the strain and a fermented yoghurt prepared by using the straincan obviously relieve constipation. Compared with the conventional commercial strain of the same type, the pediococcus pentosaceus disclosed by the invention has the advantages that an obvious bowel relaxing effect is realized; further investigation amazedly shows that the strain has a very obvious inhibiting effect on the growth of pathogenic bacteria of staphylococcus aureus; the survival rate of zebra fishes invaded by vibrio vulnificus can be obviously increased.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Pediococcus pentosaceae and its application. Background technique [0002] With the change of eating habits and the continuous acceleration of the pace of life, constipation has become a common disease among middle-aged and elderly people and office workers, and it tends to become younger. According to statistics, the incidence of constipation in my country ranges from 3.4% to 27.2%. Long-term constipation can cause carcinogens produced by the fermentation of intestinal bacteria to induce intestinal cancer. Some patients with severe constipation have affected their quality of life. However, most patients with constipation are troubled by constipation, rarely go to the hospital, and pay less attention to it. Some patients take laxatives or traditional Chinese medicine enemas in order to solve the difficulty in defecation, but this will cause relatively large ...
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