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Seminal plasma biochemical zinc ion rapid detection reagent strip and detection method

A technology for detecting reagents and zinc ions, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators. It can solve the problems of time-consuming, laborious and cumbersome operations, and achieve accurate measurement results Discoloration is obvious, easy to visually distinguish and machine-readable

Active Publication Date: 2021-08-24
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biochemical testing of seminal plasma in the reproductive centers of major hospitals adopts the traditional microplate reader test, which is cumbersome, time-consuming and laborious, and the report takes up to one week, which cannot meet the needs of timely diagnosis.

Method used

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  • Seminal plasma biochemical zinc ion rapid detection reagent strip and detection method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation steps of the seminal plasma biochemical zinc ion rapid detection reagent strip include:

[0034] 1) Configure solution A, which contains:

[0035] 4-(2-pyridylazo)-resorcinol sodium salt: 3g

[0036] Gelatin: 100g,

[0037] Polyethylene glycol: 12g,

[0038] Sodium citrate: 13g,

[0039] Sodium azide: 8g,

[0040] Tris buffer (pH=9.0) was adjusted to 1000mL;

[0041] 2) Apply solution A evenly on the plastic base film, and dry for 10 minutes to form a color-developing layer;

[0042] 3) Laying a nitrocellulose membrane on the chromogenic layer to form a polymer membrane, and drying in a low-humidity environment to obtain a reagent strip.

[0043] The test paper was used for the biochemical zinc ion test of seminal plasma, and the test results showed that it had good color development effect and obvious gradient.

Embodiment 2

[0045] The preparation steps of the seminal plasma biochemical zinc ion rapid detection reagent strip include:

[0046] 1) Configure solution A, which contains:

[0047] 4-(2-pyridylazo)-resorcinol sodium salt: 5g

[0048] Gelatin: 100g,

[0049] Polyethylene glycol: 12g,

[0050] Sodium citrate: 13g,

[0051] Sodium azide: 8g,

[0052] Tris buffer (pH=9.0) was adjusted to 1000mL;

[0053] 2) Solution A is evenly coated on the plastic base film, and dried for 3 hours to form a color-developing layer;

[0054] 3) Configure solution B, which contains:

[0055] Gelatin: 90g

[0056] Tris buffer (pH=9.0) was adjusted to 1000 mL.

[0057] 4) Apply solution B evenly on the color-developing layer;

[0058] 5) After liquid B is dried for 15 minutes, a shielding film is formed, and a nitrocellulose film is laid on it to form a polymer film, and dried in a low-humidity environment;

[0059] 6) Lay the hydrophilic nylon membrane on the nitrocellulose membrane, cut it into a spe...

Embodiment 3

[0062] A method for rapid detection of seminal plasma biochemical zinc ions, comprising the following steps:

[0063] 1) Configure 0mmol / L, 0.625mmol / L, 1.25mmol / L, 2.5mmol / L, 5.0mmol / L, 7.5mmol / L, 10mmol / L zinc ion standard solution;

[0064] 2) the above-mentioned 7 kinds of standard solutions were added dropwise to the seminal plasma biochemical zinc ion test strip prepared in Example 2;

[0065] 3) Use a dry chemical analyzer to measure the reflectance absorbance for 1 minute, and draw a standard curve based on 7 points, such as figure 1 ;

[0066]4) When detecting the sample, the sample is added to the seminal plasma biochemical test strip prepared according to Example 2, and after 1 minute, the dry chemical analyzer is used to measure the reflection absorbance, and the measured reflection absorbance and the corresponding concentration on the standard curve are The zinc ion concentration of the sample solution to be tested.

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Abstract

The invention discloses a reagent strip and a detection method for rapid detection of seminal plasma biochemical zinc ions. The reagent strip is prepared by the following method: 1) uniformly coating a coating liquid on a transparent base film, and forming a color-developing layer after drying; 2) Paste the polymer film on the chromogenic layer to prepare a reagent strip. Wherein, the film coating solution includes buffer, developer, film-forming agent, surfactant, masking agent and preservative. The seminal plasma biochemical zinc ion rapid detection test strip prepared by the present invention, under alkaline conditions, the seminal plasma zinc ion can react rapidly with the chromogenic layer in the test strip, and the reaction product has a strong absorption peak at a specific wavelength , within a certain range of zinc ion concentration, the test strip changes color significantly, is easy to visually distinguish and machine read, and is not interfered by other substances in the seminal pulp, and the preparation method of the test strip is simple and easy to operate. The method for rapid detection of seminal plasma biochemical zinc ions of the present invention adopts the test paper strip for detection, and has rapid detection, stable performance and accurate measurement results.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a seminal plasma biochemical zinc ion rapid detection reagent strip and a detection method. Background technique [0002] With the deterioration of the natural environment, the quality of male sperm generally declines. Seminal plasma biochemical examination is of great significance in evaluating the function of accessory gonads and studying the impact of accessory gonads on male fertility. Zinc ions in seminal plasma can be combined with protein and distributed on the surface of sperm to protect biofilm, which is beneficial to delay membrane lipid peroxidation and maintain sperm motility, and zinc is also a cofactor of antibacterial factor and enzyme activity. In addition, zinc has the function of stabilizing sperm nucleus chromosomes. Long-term zinc deficiency can lead to gonadal hypogenesis, hypogonadism, and decreased sperm motility and sperm density. At present, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 白鹏利陈晨南雪燕孙旭明何良王彤
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI