Field propagation method of arbuscular mycorrhizal fungi

Abstract

The invention discloses a field propagation method of arbuscular mycorrhizal fungi. The field propagation method of the arbuscular mycorrhizal fungi comprises the following steps: 1) disinfecting a propagation medium; uniformly flattening medium soil; enabling the flattened medium soil to be exposed to sun lights; spraying a 50% Bavinstin wettable powder to the medium soil; and exposing the mediumsoil to the sun till the medium soil is completely dry; 2) mutually preparing the arbuscular mycorrhizal fungi and propagation host seeds into pelleted seeds; and inoculating the pelleted seeds in the disinfected medium; and 3) after the pelleted seeds are cured for 3-4 months, obtaining the medium as a fungicide. The field propagation method of the arbuscular mycorrhizal fungi, disclosed by theinvention, has the advantages that the inoculating process is simplified and the actual operation is made simpler and more convenient by organically combining a seed pelleting technology and an arbuscular mycorrhiza inoculating technology; and through the adoption of the technology, the contact between the fungicide and the seeds is much closer, so that the application of the arbuscular mycorrhizal fungi can be reduced.

Description

technical field [0001] The invention belongs to the technical field of mycorrhizal fungi propagation, in particular to a field propagation method of arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizae (AM) fungi are the most widely distributed type of fungi among soil symbiotic fungi. Arbuscular mycorrhizal fungi can improve the biological function indicators of host plants and enhance the tolerance of host plants to adversity, so they are often used as materials for ecological restoration technologies. At present, the methods for cultivating arbuscular mycorrhizal fungi mainly include the following categories: [0003] The single-spore culture method is a method in which pure spores are isolated by semi-hydroponic technology and wet sieving separation technology, and then inoculated again on the root of the multiplication host seedling to obtain a single pure strain of bacteria. Its advantage is that the strain obtained is a single spore pro...

AI Technical Summary

Problems solved by technology

This method is relatively simple, but it is usually carried out in a sterile room by using the potted method, and due to the large particle size of the substrate and poor water retention performance, the requirements for artificial maintenance are also relatively high.

[0007] Although arbuscular mycorrhizal fungi have obvious advantages in ecological restoration technology, it is often difficult to meet the needs of practical engineering due to the limitation of their propagation and cultivation methods.