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Application of micelle as photosensitizer formed by amphiphilic molecules and Ag2S quantum dots

A technology of amphiphilic molecules and quantum dots, applied in the field of nanomaterials, can solve problems such as damage, necrosis, and phototoxicity

Active Publication Date: 2019-04-12
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the shallow treatment depth of the photosensitizer in the prior art and the technical problems of phototoxicity, and the amphiphilic molecule with good biocompatibility in the present invention and Ag 2 The micelles formed by S quantum dots, a certain concentration of the micelles are induced by near-infrared light excitation, the micelles change from the ground state to the excited state, and then react with oxygen molecules in the surrounding environment to produce toxic singlet states Oxygen, this singlet oxygen can directly induce tumor cell apoptosis or necrosis, or destroy blood vessels in the tumor interstitium to cause tumor ischemia and hypoxia, etc., and can also activate specific immune mechanisms to enhance the anti-tumor effect

Method used

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  • Application of micelle as photosensitizer formed by amphiphilic molecules and Ag2S quantum dots
  • Application of micelle as photosensitizer formed by amphiphilic molecules and Ag2S quantum dots
  • Application of micelle as photosensitizer formed by amphiphilic molecules and Ag2S quantum dots

Examples

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Embodiment 1

[0036] Example 1: Test at the probe level to generate singlet oxygen after light excitation of silver sulfide after water transfer

[0037] The use of singlet oxygen will combine with the capture agent TEMP to generate TEMPO, and TEMPO has a unique triple resonance signal peak. The Bruker electron spin resonance spectrometer was used as the detection system, the MDL-III-808-2.5W laser was used as the excitation light source, and the spectral data were collected by the control program.

[0038] Test method for singlet oxygen produced by near-infrared silver sulfide quantum dots after water transfer as a new photosensitizer probe:

[0039] Mix the silver sulfide (15μg / mL) after water transfer with the singlet oxygen scavenger TEMP (2,2,6,6-tetramethylpiperidine), and use the MDL-III-808-2.5W laser at 1.0w / cm 2 Under light-induced excitation for 10 min, the signal was detected on a Bruker electron spin resonance spectrometer.

[0040] The result is as figure 1 shown, from fig...

Embodiment 2

[0041] Example 2: Detecting the change of singlet oxygen generation over time at the probe level

[0042] DPBF is one of the most active singlet oxygen scavenger known, and singlet oxygen will attack the furan ring in the DPBF structure to open it, resulting in a change in the absorption of DPBF at 410nm. Therefore, by co-incubating DPBF with silver sulfide after water transfer, after laser irradiation for different times, the absorption spectrum was collected on a UV-2550 ultraviolet-visible spectrophotometer.

[0043] The test method for the change of singlet oxygen produced by the near-infrared silver sulfide quantum dots as a new photosensitizer probe over time after water transfer is as follows:

[0044] Take 2.5mL of silver sulfide (15μg / mL) after water transfer into a quartz cuvette, add 100μL of DPBF (1,3-diphenylisobenzofuran), and pass through MDL-III-808-2.5W laser 1.0w / cm 2 After laser induction for different times (0, 2, 12, 20, 30, 42 and 56 min), the spectra w...

Embodiment 3

[0046] Example 3: Detection of singlet oxygen production at the cellular level

[0047] DCFH-DA is known as an active oxygen fluorescence detection kit. When DCFH-DA diffuses into the cell, it will deacetylate and become non-fluorescent DCFH, and DCFH can be rapidly oxidized by reactive oxygen species to DCF with high-intensity fluorescence, and the fluorescence intensity is related to the ROS content in the cell fluid. proportional. Therefore, after HeLa cells were co-incubated with the probe after water transfer, and after the MDL-III-808-2.5W laser was excited by the light induction, the emission of different fluorescence spectra was recorded by the QE6500 fluorescence spectrometer, and different methods were observed by the fluorescence microscope. Fluorescence imaging of processed cells was performed to detect the ability of the probe to induce singlet oxygen production.

[0048] The near-infrared silver sulfide quantum dot after water transfer is used as a new photosen...

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Abstract

The invention discloses an application of a micelle as a photosensitizer formed by amphiphilic molecules and Ag2S quantum dots, and belongs to the technical field of nano materials. According to the application of a micelle as a photosensitizer formed by amphiphilic molecules and Ag2S quantum dots, the amphiphilic molecules are amphiphilic polymers or amphiphilic proteins, and include amphiphilicphospholipid molecules. Under the excitation of near-infrared light, the micelle changes from a ground state to an excited state, and reacts with surrounding oxygen molecules so as to enable the oxygen molecules to generate toxic photochemical products. The power density of the near-infrared light is 1-2.0 w / cm<2>. The micelle formed by the amphiphilic molecules and the Ag2S quantum dots in the invention has a photodynamic tumor cell-killing ability excited by the near-infrared light, has good blood compatibility, and solves the technical problems of phototoxicity and shallow treatment depth of photosensitizers in the prior art.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, in particular to amphiphilic molecules and Ag 2 Application of micelles formed by S quantum dots as photosensitizers. Background technique [0002] Cancer is one of the main reasons that threaten human life and health, so the diagnosis and treatment technology of cancer has a great social demand. As a minimally invasive treatment method, photodynamic therapy is a new technology that has developed rapidly in the field of tumor treatment in recent years, and has become one of the most active research fields in the science of tumor prevention and treatment. The basic principle is to use the photosensitizer to absorb external visible or near-infrared light energy, transform itself from the ground state to the excited state, and then react with the surrounding molecular oxygen to produce highly active excited state toxic photochemical products such as active oxygen free radicals, etc., and then...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/107A61K47/24A61P35/00
CPCA61K41/0057A61K47/24A61P35/00A61K9/1075
Inventor 赵元弟程凯刘波张晓帅安杰李呈宣扬张若筠
Owner HUAZHONG UNIV OF SCI & TECH
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