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Preparation and application of novel virus molecule imprinted fluorescent sensor

A fluorescent sensor and molecular imprinting technology, which is applied in the field of analytical chemical detection, can solve the problem of unsatisfactory imprinting effect and achieve the effect of improving specific recognition ability

Active Publication Date: 2019-05-10
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Considering the strength, specificity and directionality of the force, metal coordination as the form of force between the template molecule and the functional monomer is expected to have a positive impact on the imprinting effect. Applications of the technology have not been reported yet
[0004] Finally, considering that macromolecular substances such as viruses have larger molecular particle sizes, when using general carriers (such as the common SiO 2 , Fe 3 o 4 etc.) can leave very few imprinting sites, so the imprinting effect is not very ideal

Method used

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  • Preparation and application of novel virus molecule imprinted fluorescent sensor
  • Preparation and application of novel virus molecule imprinted fluorescent sensor
  • Preparation and application of novel virus molecule imprinted fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation method of a novel viral molecularly imprinted fluorescent sensor

[0028] (1) Preparation of metal-organic framework MIL-101: Cr(NO 3 ) 3 9H 2 O (2.00g), terephthalic acid (TPA, 0.82g) were mixed with sodium acetate solution, and after magnetic stirring for 30min, the solution was transferred to a polytetrafluoroethylene reactor and placed in a constant temperature blast drying oven at 200°C . After 12 hours, the sample was fully washed with ultrapure water and filtered, and then dried in a constant temperature drying oven at 50°C for 6 hours. Finally, MIL-101 was obtained by purification in a hot water bath and hot ethanol, followed by the addition of 30 mM NH 4 F and continue heating at 60°C for 3h. Finally the samples were collected by centrifugation and dried at 50 °C for 12 h.

[0029] (2) Preparation of vinylated MIL-101 (MIL-101@C=C): Mix 75mL absolute ethanol, 15mL ultrapure water and 0.1g MIL-101 for 20min, then add 2mL ammonia wate...

Embodiment 2

[0033] Example 2: Characterization of the performance, morphology and structure of the JVIPs fluorescent sensor and intermediate products.

[0034] The structure and morphology of all the prepared materials were characterized by Fourier transform infrared spectrometer, X-ray diffractometer and scanning electron microscope. figure 2 for MIL-101@SiO 2 (a), MIL-101@SiO 2 C=C(b), infrared spectra of JVIPs(c) and NIPs(d) particles. 500cm -1 The absorption peak at is attributed to the Cr-O of MIL-101 at 3400cm -1 The absorption here is produced by the water adsorbed by the material. at 1090cm -1 and 800cm -1 The absorption peak at is attributed to the asymmetric stretching vibration of Si-O-Si and the stretching vibration of Si-O. C=O and C-H(-CH in zinc acrylate 3 ) absorption peaks appear at 1716cm -1 and 2980cm -1 place. Comparing (c) and (d), it can be observed that the absorption bands of the imprinted polymer (after elution of the template) and the non-imprinted po...

Embodiment 3

[0037] Embodiment 3: the application of described JVIPs fluorescent sensor

[0038] The experimental conditions of this example are: the dosage of JVIP is 19 μg / mL, the pH is 7.5, the adsorption time is 20 min, and the temperature is 37°C. The specific implementation method is: take a specific concentration of JEV and 19 μg / mL of JVIP in PB buffer, adjust the pH of the whole system to 7.5, and measure the fluorescence intensity after shaking and adsorbing at 37°C for 20 minutes.

[0039] (1) Detection and analysis of different concentrations of JEV by JVIPs fluorescent sensor

[0040] According to the above experimental steps, the JVIPs sensor of the present invention is used to detect and analyze the JEV of different concentrations, the results are as follows Figure 5 As shown, the analytical concentration range of the prepared sensor for JEV is 0.05-1.4nM, and the detection limit is 0.1pM. The results show that the linear range is wide, the detection limit is low, and the ...

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Abstract

The invention discloses a molecule imprinted fluorescent sensor for detecting viruses. A metal organic framework is used as a carrier material of the sensor, a metal chelator is used as a functional monomer, and a six-membered ring is formed between a template molecule and the functional monomer through metal chelation, so as to prepare the sensor. Specific identification capability of the sensorfor template viruses is improved through deactivation. The metal organic framework has high specific surface area, and can provide more imprinting sites, improving response sensitivity of the sensor.Deactivation lowers non-specific adsorption, so that selectivity of the sensor for the template molecule is improved; metal coordination reduces interference of covalent force to template elution andfixes template molecule more effectively relative to non-covalent force; the fluorescent analysis method has advantages of high flexibility, high selectivity, convenience in operation, and so on. Compared with other analysis methods or sensors, the molecule imprinted fluorescent sensor prepared in the invention has high selectivity for template molecules and high detection flexibility, and has high practical application value and significance in biosensing and virus detection and prevention.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry detection, and in particular relates to the preparation and application of a novel virus molecular imprinted fluorescent sensor. Background technique [0002] Non-specific binding is a problem often encountered in the field of separation and detection, and the existence of this phenomenon is not conducive to the specific recognition and detection of target molecules. Over the years, researchers have developed a variety of strategies to solve this problem with some success. These successful cases include bifunctional chains and self-assembled monolayer strategies (SAM) [Mrksich M., Chem. or Passivation strategy (i.e. pre-adsorption saturation by strong attachment proteins such as bovine serum albumin (BSA), Tween 20, polyethylene glycol (PEG), casein, milk protein, etc. [Gast M., Kahner S. , Sobek H., Walther P., MizaikoffB., Analytical Chemistry, 2018, 9.], etc. In the passivation ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 蔡昌群罗谅晖冯文宝陈小明
Owner XIANGTAN UNIV
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