Unlock instant, AI-driven research and patent intelligence for your innovation.

A three-in-one test card for simultaneously detecting uric acid, creatinine, and urea in a sample and its preparation method

A three-in-one, in-sample technology, applied in the field of medical testing, can solve problems such as difficulty in realizing large-scale structure and formula, inconvenience for users or patients, and complicated operation, and achieve the effects of average flow rate, simple use, and uniform distribution

Active Publication Date: 2022-02-18
杭州联晟生物科技有限公司
View PDF12 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the detection of renal function indicators in hospitals or clinics mainly involves the detection of three commonly used indicators such as uric acid, creatinine, and urea, and the methods used are basically wet detection, using kits and large biochemical instruments. The time is long and the operation is complicated. Professional operation
cause some inconvenience to users or patients
As for the indicator of dry method detection, only one company has the relevant product registration certificate, and no mature product is on the market, so the market is basically blank. Its related patents include CN106645128A, CN106645758A, and CN106645675A. The patents are all for testing a single item, and from the structure and It is difficult to achieve scale in terms of formula; the present invention solves such problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A three-in-one test card for simultaneously detecting uric acid, creatinine, and urea in a sample and its preparation method
  • A three-in-one test card for simultaneously detecting uric acid, creatinine, and urea in a sample and its preparation method
  • A three-in-one test card for simultaneously detecting uric acid, creatinine, and urea in a sample and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0057] A method for preparing a three-in-one test card for simultaneously detecting uric acid, creatinine, and urea in a sample, comprising the following steps:

[0058] Prepare uric acid reaction solution, the formula of uric acid reaction solution includes: 5-50KU / L urate oxidase, 1-20KU / L ascorbate oxidase, 1-20KU / L peroxidase, 0.1-10g / L film-forming agent, 10 -100g / L stabilizer, 0.1-10g / L surfactant, 0.1-10g / L chromogenic substance, 0.1-1M buffer; stir for 1 hour before use;

[0059] Preparation of creatinine reaction solution includes: 5-50KU / L creatinase, 5-100KU / L sarcosine oxidase, 5-100KU / L creatine hydrolase, 1-20KU / L ascorbate oxidase, 1-20KU / L over Oxidase, 0.1-10g / L film-forming agent, 10-100g / L stabilizer, 0.1-10g / L surfactant, 0.1-10g / L chromogenic substance, 0.1-1M buffer solution; use;

[0060] The preparation of urea reaction solution includes: 5-100KU / L urease, 0.1-10g / L film-forming agent, 10-100g / L stabilizer, 0.1-10g / L surfactant, 0.1-10g / L chromogenic ...

Embodiment 1

[0068] The preparation method of finished product 1 comprises the following steps:

[0069] Step 1, prepare uric acid reaction solution: mix 10KU uric acid oxidase, 3KU ascorbate oxidase, 1KU peroxidase, 0.5g polyvinyl alcohol, 10g bovine serum albumin, 3g trehalose, 0.5gtritonX-405, 1g N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt, 2g of 4-aminoantipyrine was added to 1L of 0.1MPH phosphate buffer solution with a pH value of 7.2 Stir in medium for one hour and set aside;

[0070] .Step 2, preparation of creatinine reaction solution: 10KU creatinase, 200KU sarcosine oxidase, 10KU creatine hydrolase, 2KU ascorbate oxidase, 1KU peroxidase, 0.5g polyvinyl alcohol, 10g bovine serum white Egg white, 3g treacle sugar, 0.5gtritonX-405, 1g N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt, 2g 4-aminoantipyrine Add it to 1L of 0.1MPH phosphate buffer solution with a pH value of 7.2 and stir for one hour before use;

[0071] .Step 3, prepare ur...

Embodiment 3

[0085] The manufacturing method of finished product 3 comprises the following steps:

[0086] Step 1, prepare uric acid reaction solution: mix 40KU uric acid oxidase, 15KU ascorbate oxidase, 10KU peroxidase, 5g polyvinyl alcohol, 50g bovine serum albumin, 50g treparin, 5gtritonX-405, 5g N- Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt, 5g of 4-aminoantipyrine was added to 1L of 0.1MPH phosphate buffer with a value of 7.2 and stirred Standby in one hour;

[0087] .Step 2, preparation of creatinine reaction solution: 40KU creatinase, 40KU sarcosine oxidase, 50KU creatine hydrolase, 10KU ascorbate oxidase, 5KU peroxidase, 5g polyvinyl alcohol, 50g bovine serum albumin ,50g sea bath sugar, 5gtritonX-405, 5g N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt, 5g4-aminoantipyrine was added to 1L of 0.1MPH phosphate buffer solution with a pH value of 7.2 was stirred for one hour before use;

[0088] .Step 3, prepare urea reaction solution: 50KU...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a three-in-one test card for simultaneous detection of uric acid, creatinine and urea in a sample, an upper case 700, a lower case 100, a sample injection hole 701 arranged on the upper case 700, and a hole 701 arranged on the lower case 100. The bottom plate at the upper end, the uric acid detection hole 101 on the lower shell 100, the creatinine detection hole 100 on the lower shell, the urea detection hole 100 on the lower shell, the uric acid detection hole 201 on the bottom plate, the creatinine detection hole 202 on the bottom plate, Base plate urea detection hole 203, uric acid reaction layer 301, creatinine reaction layer 302, and urea reaction layer 303 arranged on the upper end of the base plate detection hole; the present invention improves the test card from the formula and structure, so that the test card can be used together with the dry biochemical analyzer It can quickly and quantitatively detect the content of uric acid / creatinine / urea in whole blood, serum / plasma, and other body fluids in humans or animals; the test results match well with hospital test results. The invention is simple to use and does not require professional operation.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a three-in-one test card for simultaneously detecting uric acid, creatinine and urea in a sample and a preparation method thereof. Background technique [0002] Renal function refers to the function of the kidneys to excrete metabolic waste in the body, maintain the stability of the body's sodium, potassium, calcium and other electrolytes, and acid-base balance. Renal function tests include creatinine, urea, blood and urine β2-microglobulin, urine Albumin, urinary immunoglobulin G, urinary secretory immunoglobulin A, etc. [0003] The kidneys have three basic functions: (1) generate urine and excrete metabolites. The body produces a variety of wastes during the metabolic process, most of which are excreted through glomerular hemofiltration and renal tubule secretion with urine. (2) Maintain body fluid balance and acid-base balance in the body. The kidney excretes excess water ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 陈军刚
Owner 杭州联晟生物科技有限公司