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Penicillium asturianum and its inocula and application thereof

A technology of Penicillium aspenicillium and strains, applied in the direction of application, fungicide, biocide, etc., can solve problems such as calcareous soil, salinization, plant diseases, etc., achieve high production efficiency, improve resistance, and simple production process Effect

Active Publication Date: 2019-07-02
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by this invention is to provide a kind of Penicillium aspenicillium to solve problems such as plant disease, salinization and calcareous soil

Method used

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  • Penicillium asturianum and its inocula and application thereof
  • Penicillium asturianum and its inocula and application thereof
  • Penicillium asturianum and its inocula and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Screening and identification of P. asturianum XK-12

[0043] P.asturianumXK-12 is a bacterial strain selected and bred by the inventor from strawberry rhizosphere soil (based on the antagonistic effect of Fusarium oxysporum and resistance to salt stress). The screening process is as follows:

[0044] Dissolve the collected soil samples in sterile water, shake and mix well. The sample solution was serially diluted, taking 10 -4 、10 -5 、10 -6 The dilutions of three concentration gradients were evenly spread on the PDA medium (medium composition: potato 200g, glucose 20g, agar 20g, sodium chloride 100g distilled water 1000mL) containing 20% ​​NaCl. Place it in a constant temperature incubator at 28°C for 3-5 days, transfer a small number of colonies grown to the edge of a blank PDA plate, place Fusarium oxysporum as an indicator bacterium in the middle, observe the characteristics of the colonies, and use the antagonistic effect as the screening target. A si...

Embodiment 2

[0050] Embodiment 2: P.asturianumXK-12 bacterial strain biological characteristic

[0051] The P. asturianumXK-12 stored on the slant at 4°C was activated and inoculated on PDA medium (ie, the activation medium). The activation temperature was 28°C and the activation time was 2 days. Use a 0.8cm hole punch to transfer fresh bacteria cakes to empty inorganic phosphorus medium A, organic phosphorus medium B, cellulose Congo red medium C, pH8-11PDA medium (D-G) and containing 5%-20% NaCl PDA medium (H-J) was cultured at 28°C for 5 days to observe the colony morphology.

[0052] Inorganic phosphorus medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g,

[0053] MgSO 4 ·7H 2 O 0.3g, CO 3 (PO4) 2 10g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, distilled water 1000mL, pH 7.0~7.5;

[0054] Organic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, yeast extract powder 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate 0.3g, ferrous sul...

Embodiment 3

[0059] Example 3: Plate confrontation experiment of P.asturianumXK-12 against fungal disease pathogens

[0060] P. asturianum XK-12 and 6 kinds of pathogenic fungi (Colletotrichumgloeosporioides, Neofusicoccumkwambonambiense, Fusariunoxysporun, Phytopythiumhelicoide, Ceratobasidium sp.AG-A and Fusariumsolani) were inoculated on PDA at the same time, and cultured at 28°C for three days. tablet, antagonistic effects such as Figure 4 As shown, the inhibitory rate to Neofusicoccumkwambonambiense reaches 95%, and the antagonistic effect to Ceratobasidium sp.AG-A reaches 45%. The experiment proves that the strain has a broad-spectrum disease-resistant effect.

[0061] Mycelia growth inhibition rate (%)=(blank control colony growth diameter-treatment colony growth diameter) / blank control colony growth diameter×100

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Abstract

The invention discloses penicillium asturianum, which is classified as penicillium asturianum and has a strain number XK-12, and is preserved in the General Microorganisms Collection and Management Center of China, a preservation time is March 28th, 2019, and a preservation number is CGMCC No. 17198. The invention also discloses an application of the above-mentioned penicillium asturianum. The strain of the invention has excellent properties such as high temperature resistance, salt and alkali resistance, cellulase production, iron absorption and phosphorus solubilization, and can be applied to poor soil, saline-alkali land and calcareous soil restoration, straw decomposing and composting agent inoculation. The P. asturianum XK-12 strain has large sporulation quantity, is highly efficientand has the broad-spectrum plant disease biocontrol ability, can promote plant growth and is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of microbial bacterial agents, in particular to a P. asturianum, bacterial agents and applications thereof. Background technique [0002] P. asturianum XK-12 belongs to the phylum Ascomycota, the class Eurotiomycetes, the order Eurotiales, and the family Trichocomaceae. The strain has a biosafety risk level of 1, and has excellent characteristics such as saline-alkali tolerance, high phosphorus solubility, high-yield cellulase decomposing straw, and significant antagonistic effect on broad-spectrum plant diseases. As an organic fertilizer returning to the field in situ, microbial antibacterial agents and their metabolites have broad application prospects in biopesticides and other aspects. The strain has a high spore production rate, can produce spores while growing, has a high level of solid-state fermentation and liquid fermentation, and is simple in process, suitable for industrial production. Balance and so on h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G3/00C05G3/04C05F11/08A01N63/04A01N37/46A01P3/00A01P21/00C09K17/14C12R1/80C09K101/00C05G3/80
CPCC05G3/00C05F11/08A01N37/46C09K17/14C09K2101/00C05G3/80C12R2001/80C12N1/145A01N63/30
Inventor 徐虹詹伊婧彭雯杰冯小海许宗奇王明轩
Owner NANJING UNIV OF TECH
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