Flexible liposome cosmetic containing biomacromolecules and preparation method of flexible liposome cosmetic containing biomacromolecules

A technology of biological macromolecules and flexible liposomes, which is applied in cosmetic preparations, medical preparations containing active ingredients, cosmetics, etc., can solve the problems of nutrient factor retention, repair skin damage, improve the effect, and promote wound healing. healing effect

Active Publication Date: 2019-07-05
深圳高尚科美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cosmetics currently on the market claim to add a variety of growth factors, nutrients, etc., but in fact, none of them really solve the problem of skin absorption. These nutritional factors only stay on the surface of the skin, and can only solve the problems on the surface of the skin for a short time, and cannot go directly to the deep layer of the skin. , fundamentally improve the level of cell activation and delay skin aging

Method used

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  • Flexible liposome cosmetic containing biomacromolecules and preparation method of flexible liposome cosmetic containing biomacromolecules
  • Flexible liposome cosmetic containing biomacromolecules and preparation method of flexible liposome cosmetic containing biomacromolecules
  • Flexible liposome cosmetic containing biomacromolecules and preparation method of flexible liposome cosmetic containing biomacromolecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1 The preparation of the flexible liposome containing biomacromolecule of the present invention

[0065] Accurately weigh soybean lecithin, sodium deoxycholate or polysorbate 80 or its derivatives, and vitamin E according to the formula, dissolve them in chloroform / ethanol or ethanol respectively, dissolve and mix them in a 250ml pear-shaped bottle, and rotate to evaporate at room temperature for 2 The resulting film was placed in a vacuum oven overnight. The next day, distilled water and appropriate amount of hydrophobized modified polypeptide were added, and a 400w probe was sonicated for 30 minutes to obtain a blank transfersome solution. Slowly add an appropriate amount of bFGF (or EGF, SOD, etc.), and after incubating at room temperature for 40 minutes, filter the resulting mother liquor with a 0.2 μm polycarbonate membrane several times, add excipient 5% mannitol, and distribute it in vials. The flexible liposome containing biological macromolecules of...

Embodiment 2

[0168] Example 2 Verification of in vitro transdermal effect of flexible liposomes containing biomacromolecules of the present invention

[0169] The in vitro transdermal experiment adopts a modified single-chamber Franz diffusion cell, and mouse skin is used as the in vitro transdermal experimental skin to compare the transdermal absorption results of different flexible liposomes containing biomacromolecules, and samples are taken at different time points. Qualitative detection by SDS-PAGE, Western Blot method, or ELISA method to measure the concentration of the drug in the transdermal receiving solution, and calculate the cumulative transdermal amount.

[0170] Test example 2-1 Qualitative verification of the transdermal effect of the flexible liposome comprising bFGF

[0171] This test example uses protein electrophoresis and Western Blot of corresponding antibody to qualitatively measure the transdermal effect of flexible liposomes modified by DP7-C comprising bFGF.

[01...

Embodiment 3

[0219] Example 3 Verification of in vivo transdermal effect of flexible liposomes containing biological macromolecules of the present invention

[0220] This embodiment uses immunohistochemistry to determine the in vivo transdermal effect of the DP7-C modified flexible liposome containing bFGF in mouse skin.

[0221] 1. Materials

[0222] Sample: the lyophilized powder of the bFGF-containing flexible liposome (1-1) prepared by Test Example 1 in Example 1;

[0223] Animals: Balb / c mice, 6-8 weeks old, female;

[0224] Reagents: Anti-bFGF antibody was purchased from Abcam Company, ready-to-use mouse SABC-AP immunohistochemistry kit.

[0225] 2. Method

[0226] The freeze-dried powder of flexible liposome containing bFGF was dissolved in distilled water and applied on the skin of depilated mice, and then sacrificed 8 hours later. The skin that had been smeared with samples was peeled off, fixed, embedded in wax block, sectioned, and immunohistochemical method was used to dete...

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Abstract

The invention belongs to the field of cosmetics, and particularly relates to a flexible liposome cosmetic containing biomacromolecules and a preparation method of the flexible liposome cosmetic containing biomacromolecules. Due to barrier action of a horny layer of epidermis of skin, most of active components of the cosmetic containing biomacromolecules difficultly permeate the skin, and in accordance with the problems, the invention provides the flexible liposome cosmetic containing biomacromolecules. The flexible liposome modified by hydrophobization modified polypeptide DP7-C or PAL-DP7 andcontaining biomacromolecules is prepared into the cosmetic, the transmissivity of the biomacromolecules in the cosmetic is effectively increased, a cosmetic easy to absorb and good in effects of resisting oxidation and resisting ageing is developed, and the application prospect is good.

Description

technical field [0001] The invention belongs to the field of cosmetics, in particular to a flexible liposome cosmetic containing biological macromolecules and a preparation method thereof. Background technique [0002] In recent years, genetic engineering products have been more and more widely used in the field of beauty and skin care, and more than ten kinds of genetic engineering products have been used in the world for slimming and shaping, skin repair and other aspects. Biomacromolecules, especially cell growth factors, play an important role in beauty and skin care. These cell growth factors originally exist in skin cells. With age, skin metabolism slows down, cell growth factor synthesis decreases, and skin gradually loses Resilience and vitality. Cell growth factors are produced by DNA recombination technology and added to cosmetics at a certain effective concentration, which can effectively solve the above problems, the cells are activated, and the collagen content...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/66A61K8/64A61K8/63A61K8/55A61K8/14A61Q19/00A61Q19/08
CPCA61K8/64A61K8/66A61K8/553A61K8/63A61K8/14A61Q19/00A61Q19/08A61K2800/56A61K2800/85
Inventor 杨莉魏于全
Owner 深圳高尚科美生物科技有限公司
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