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Pickering emulsion polymerization based imidazole antifungal drug molecular imprinted functionalized microsphere, and amplification preparation method and application thereof

A Pickering emulsion and antifungal technology, which is applied to the preparation of microspheres, microcapsule preparations, etc., can solve problems such as being unfavorable for high-quality large-scale production, and achieve the effects of simple and reliable preparation method, simple equipment and low cost.

Inactive Publication Date: 2019-08-06
LISHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported techniques for the preparation of Pickering emulsions mostly use methods such as vortex, ultrasound, oscillation and manual shaking, which are not conducive to high-quality large-scale production

Method used

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  • Pickering emulsion polymerization based imidazole antifungal drug molecular imprinted functionalized microsphere, and amplification preparation method and application thereof
  • Pickering emulsion polymerization based imidazole antifungal drug molecular imprinted functionalized microsphere, and amplification preparation method and application thereof
  • Pickering emulsion polymerization based imidazole antifungal drug molecular imprinted functionalized microsphere, and amplification preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Optimization of Preparation Conditions and Morphological Characterization of Pickering Emulsion Polymerized Molecularly Imprinted Microspheres

[0029] (1) 3.855g (15mmol) substituted template molecule [1-(2,4-dichlorophenyl)-2-(1-H-imidazole)ethanol)], 5.3mL (60mmol) functional monomer (methyl Acrylic acid), 57.0mL (300mmol) crosslinking agent (ethylene glycol dimethacrylate), and 600mg initiator (azobisisobutyronitrile) were dissolved in 56.0mL porogen (toluene), and ultrasonically mixed for 5 -15min to obtain a pre-polymerization solution.

[0030] (2) Ultrasonic dispersion of 450 mg of silica nanoparticles in 150.0 mL of Triton X-100 aqueous solution with a mass concentration of 0.2% to obtain an aqueous phase dispersion.

[0031] (3) Mix the above-mentioned pre-polymerization solution and the aqueous phase dispersion liquid, disperse the polymer Pickering emulsion by using a high-speed shear emulsifier, and seal it under a nitrogen atmosphere.

[0032] ...

Embodiment 2

[0037] Example 2 Evaluation of Adsorption Performance of Pickering Emulsion Imprinted Polymer Microspheres on Imidazole Antifungal Drugs (1) Adsorption Isotherm Curve and Scatchard Analysis

[0038] Use acetonitrile as solvent to prepare a series of standard solutions of Campanola, with a concentration range of 0.005-4.0mM; weigh 20mg of imprinted or non-imprinted microspheres into a 10mL volumetric flask, add 2mL of standard solutions of different concentrations, seal and shake at 150rpm (25°C) 24h to reach adsorption equilibrium. After filtration, the supernatant was taken for liquid chromatography determination. The calculation formulas of adsorption capacity and adsorption equilibrium constant are:

[0039]

[0040] product; m (g) is the mass of imprinted or non-imprinted microspheres used; Q and Q max (μmol g -1 ) are the adsorption amounts of campanola in equilibrium and saturated adsorption, respectively. Using the above calculated data to make adsorption isother...

Embodiment 3

[0043] Example 3 Pickering emulsion imprinted microspheres are used for matrix solid phase dispersion extraction purification of imidazole antifungals in fish meat

[0044] The fish used in the experiment were collected from the local market. The bones and skin of the fish were removed, and the fish meat was mashed with a tissue grinder and stored at -20°C. Weigh 250 mg imprinted microsphere material and place it in a glass mortar, weigh 200 mg fish sample and place it on top of the material, add an appropriate volume of imidazole antifungal drug standard sample to the fish sample, and place it in a dark place at room temperature for 60 min. The control group was treated in the same way as above, except that the imprinted material was replaced with non-imprinted material. After standing still, add 200 μL of water into the glass mortar, mix and grind for 5 minutes to form a uniform mixture of the material and the fish sample. The homogeneous mixture was transferred to a 3 mL s...

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Abstract

The invention provides a Pickering emulsion polymerization based imidazole antifungal drug molecular imprinted functionalized microsphere, and an amplification preparation method and application thereof. The method includes dissolving an alternative template molecule, a cross-linking agent, a functional monomer and an initiator into methylbenzene, and obtaining a pre-polymerization solution through ultrasonic uniform mixing; ultrasonically dispersing silica nanoparticles into a Triton X-100 aqueous solution to obtain aqueous phase dispersion liquid; and performing mixing and emulsification onthe pre-polymerization solution and the aqueous phase dispersion liquid through a high-speed shearing emulsifying machine so that polymerization emulsion with the particle sizes of liquid drops being10-100 [mu]m can be obtained, performing sealing with a temperature of 55-75 DEG C under a nitrogen atmosphere to perform polymerization for 12-48 h, and removing the nanoparticles on the surface of obtained polymer microsphere so that the imidazole antifungal drug molecular imprinted functionalized microsphere can be obtained. The preparation method has characteristics of being simple in step, large in output and high in yield; and the obtained imprinted polymer microsphere is good in selectivity, high in specific surface area, uniform in particle size distribution and suitable for preparingchromatographic packings, solid phase extraction packings or pollutant adsorption and removal materials.

Description

technical field [0001] The invention relates to a molecularly imprinted functionalized microsphere of imidazole antifungal drugs based on Pickering emulsion polymerization, an enlarged preparation method and application thereof. Background technique [0002] Molecularly imprinted polymers refer to polymers prepared with target molecules as templates under the joint action of crosslinking agents and monomers. The polymer materials have strong specific selectivity for target molecules. The preparation methods of molecularly imprinted polymer materials mainly include: bulk polymerization, emulsion polymerization, precipitation polymerization, suspension polymerization and in-situ polymerization. Among them, bulk polymerization, which is easy to prepare and has many applicable systems, is the most widely used. For example, molecularly imprinted solid-phase extraction materials, which have developed rapidly in recent years, are mostly prepared by bulk polymerization. However, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F222/14C08J9/26B01J13/14
CPCB01J13/14C08F220/06C08J9/26C08F222/102
Inventor 孙晓丽王慕华彭俊钰杨甲甲李江峰
Owner LISHUI UNIV
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