Multi-level hole function support material for mobilization endogenous neural stem cell repair spinal cord injuries and preparation method and application thereof

A technology of neural stem cells and scaffold materials, applied in the field of multi-level porous functional scaffold materials and their preparation, to achieve good mechanical strength, promote the recovery of motor function, and promote the recovery of lower limb motor function

Active Publication Date: 2019-08-09
SHANGHAI TONGJI HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no chitosan-based scaffold material that contains guide holes along the long axis of the material to provide the spatial structure required for neural circuit reconstruction to facilitate the directional migration of endogenous neural stem cells

Method used

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  • Multi-level hole function support material for mobilization endogenous neural stem cell repair spinal cord injuries and preparation method and application thereof
  • Multi-level hole function support material for mobilization endogenous neural stem cell repair spinal cord injuries and preparation method and application thereof
  • Multi-level hole function support material for mobilization endogenous neural stem cell repair spinal cord injuries and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of the multi-level porous functional scaffold material of the present invention (1)

[0052] 1. Chitosan grafted double bond:

[0053] Weigh 2.0 g of chitosan into a three-necked flask, add 150 ml of 2% acetic acid aqueous solution, and dropwise add a small amount of KOH aqueous solution to adjust the pH to 3.8. Then, 3.51 g of glycidyl methacrylate (GMA) was slowly added dropwise, continuously stirred, and reacted at 60° C. for 6 hours. Then pour the reaction mixture into acetone to obtain a large amount of flocculent precipitate, then pour the flocculent into acetonitrile to obtain a powdery precipitate, and finally wash with acetone several times and dissolve it in water, then put it into a dialysis bag with a molecular weight of 3500 Dialyzed for 3 days and freeze-dried to obtain the raw material Chitosan-GMA, which was set aside. The chitosan grafted with double bonds was characterized by infrared spectroscopy and H-NMR.

[0054] result: f...

Embodiment 2

[0066] Example 2 Preparation of the multi-level porous functional scaffold material of the present invention (2)

[0067] 1. Chitosan grafted double bond:

[0068] With embodiment 1.

[0069] 2. Preparation of materials:

[0070] Weigh 50mg Chitosan-GMA into a small glass bottle, then add 25μL BIS (N,N'-methylenebisacrylamide, 10mg / mL) crosslinking agent (0.5% of the monomer), then add 2mg photoinitiator 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone (4% of the monomer), and finally add 1700 μL H 2 O, stirred at 37°C for 2 hours to dissolve it to form a precursor, that is, a gel-forming precursor.

[0071] After the dissolution is complete, the gel-forming precursor solution is centrifuged at 4500r / min for 4min to remove air bubbles. The solution is then injected into the capillary using an ear wash bulb.

[0072] The capillary injected with the solution was placed in liquid nitrogen at a constant speed (2.5 mm / s) using a lifter for freezing guidance. Immediately a...

Embodiment 3

[0073] Example 3 Preparation of multi-level porous functional scaffold material of the present invention (3)

[0074] 1. Chitosan grafted double bond:

[0075] With embodiment 1.

[0076] 2. Preparation of materials:

[0077] Weigh 50mg Chitosan-GMA into a small glass bottle, then add 50μL BIS (N,N'-methylenebisacrylamide, 10mg / mL) crosslinking agent (1% of the monomer), then add 1mg photoinitiator 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone (2% of the monomer), and finally add 2200 μL H 2 O, stirred at 37°C for 2 hours to dissolve it to form a precursor, that is, a gel-forming precursor.

[0078] After the dissolution is complete, the gel-forming precursor solution is centrifuged at 4500r / min for 4min to remove air bubbles. The solution is then injected into the capillary using an ear wash bulb.

[0079] Place the capillary injected with the solution in liquid nitrogen at a constant speed (3.5mm / s) for freezing guidance. Immediately after the guidance, the capi...

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Abstract

The invention relates to a multi-level hole function support material for mobilization endogenous neural stem cell repair spinal cord injuries and a preparation method and application thereof. The preparation method of the material comprises the steps that modified double-bounded chitosan is mixed with a cross-linking agent BIS, 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one anddistilled water to obtain a precursor solution, growth factors are added or not added to prepare a glue precursor solution, the precursor solution is placed into liquid nitrogen for ice crystal guiding,guiding, and then under ultraviolet irradiation at the temperature of subzero 20 DEG C, glue is formed by initiation. The material is provided with guiding holes of different sizes in the inner diameter of the long axis of the material, the space structure needed by neural circuit reconstruction is supplied, endogenous neural stem cells can be directionally migrated to the material transplanting region conveniently to form new born nerve cells, a neural network is formed by multi-level structure growth designed at the nerve synapse edge, the neural circuit at the injured region is rebuilt,and the motor function recovery after spinal cord injuries is promoted.

Description

technical field [0001] The invention relates to the technical field of nerve regeneration and repair, in particular to a multi-level porous functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injuries, a preparation method and application thereof. Background technique [0002] Spinal cord injury (SCI) usually refers to the direct injury of the spinal cord caused by various reasons such as trauma, resulting in the loss of motor and sensory functions below the level of injury to varying degrees, and even complete loss of motor and sensory functions leading to paraplegia. SCI has a high morbidity rate. In addition to causing quadriplegia and hemiplegia, it may also produce other secondary diseases, which seriously affect the quality of life of patients. The treatment and rehabilitation of SCI has always been one of the major problems in the field of basic and clinical medicine. [0003] Spinal cord injury often leads to continuous los...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/54A61L27/26A61L27/36C08F251/00C08F222/38C08F2/48
CPCA61L27/26A61L27/3675A61L27/54A61L27/56A61L2300/602A61L2430/00C08F2/48C08F251/00C08L5/08C08L51/02C08F222/385
Inventor 程黎明王启刚程和丽徐委
Owner SHANGHAI TONGJI HOSPITAL
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