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Vaginal matrix material and preparation method thereof

A matrix material and vaginal technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems that the immune system cannot remove bacteria, cannot be effectively treated, and cells cannot grow into it, so as to improve physical and mechanical properties and degradation resistance , low requirements on equipment and reaction conditions, good antibacterial and anti-infection performance

Inactive Publication Date: 2019-08-20
吴海玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been found that biological materials left in the human body for a long time or a short time often cause various bacterial infections. Once these infections occur, they cannot be effectively treated even with hundreds of times the normal dose of drugs, and the body's own immune system cannot clear them. bacteria
Second, there is a risk of degradation of this material during use
[0005] The extracellular matrix made by the method described in the existing patent CN1284265A has a high concentration of lye, a long soaking time, and a high treatment temperature, which will cause fiber digestion, collapse and void layers, which will cause cells to fail to grow into the product when the product is applied. , affecting the use of the final product
The lye concentration is low, the soaking time is short, and the treatment temperature is low, which will cause the cells to be unclean, cause the immune reaction of the matrix, and cause the product to fail to use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of a vaginal matrix material, comprising the steps of:

[0041]Collection and sterilization of raw materials in step S1: take the vaginal wall tissue material clipped during the pelvic floor reconstruction operation, chop it up, wash it, filter it dry, and sterilize it with γ-ray radiation to make it sterile; the chopping up is chopping to a size of 1cm×0.5cm;

[0042] Initial treatment of raw materials in step S2: adding the tissue material prepared in step S1 into water or saline, homogenizing, centrifuging, discarding the supernatant, and then using biological buffer for 3 times, and drying to obtain the lower tissue; The biological buffer is a mixture of DIPSO buffer and Tris buffer at a mass ratio of 1:3

[0043] Step S3 decellularization treatment: place the lower layer tissue prepared in step S2 in the decellularization solution, perform ultrasonic decellularization treatment in a dual-frequency ultrasonic environment, the low-frequency freq...

Embodiment 2

[0053] A preparation method of a vaginal matrix material, comprising the steps of:

[0054] Collection and sterilization of raw materials in step S1: take the vaginal wall tissue material clipped during the pelvic floor reconstruction operation, chop it up, wash it, filter it dry, and sterilize it with γ-ray radiation to make it sterile; the chopping up is chopping to a size of 1.5cm×0.6cm;

[0055] Initial treatment of raw materials in step S2: adding the tissue material prepared in step S1 into water or saline, homogenizing, centrifuging, discarding the supernatant, and then using biological buffer 4 times, and then drying to obtain the lower tissue; The biological buffer is mixed with DIPSO buffer and Tris buffer at a mass ratio of 1:3.5;

[0056] Step S3 decellularization treatment: place the lower layer tissue prepared in step S2 in the decellularization solution, perform ultrasonic decellularization treatment in a dual-frequency ultrasonic environment, the low-frequency...

Embodiment 3

[0066] A preparation method of a vaginal matrix material, comprising the steps of:

[0067] Collection and sterilization of raw materials in step S1: take the vaginal wall tissue material clipped during the pelvic floor reconstruction operation, chop it up, wash it, filter it dry, and sterilize it with γ-ray radiation to make it sterile; the chopping up is chopping to a size of 2cm×0.8cm;

[0068] Initial treatment of raw materials in step S2: adding the tissue material prepared in step S1 into water or saline, homogenizing, centrifuging, discarding the supernatant, and then using biological buffer 4 times, and then drying to obtain the lower tissue; The biological buffer is made by mixing DIPSO buffer and Tris buffer at a mass ratio of 1:4;

[0069] Step S3 decellularization treatment: place the lower layer tissue prepared in step S2 in the decellularization solution, perform ultrasonic decellularization treatment in a dual-frequency ultrasonic environment, the low-frequency...

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PUM

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Abstract

The invention discloses a vaginal matrix material and a preparation method thereof. The preparation method comprises the following steps: step 1, collection and sterilization of raw materials; step 2,initial treatment of raw materials; step 3, decellularization treatment; step 4, cleaning; and step 5, antimicrobial modification. The decellularizing solution used in the process of decellularization treatment is prepared by evenly mixing the following components in percentages by mass: 0.3%-0.5% of amino trimethylene phosphonic acid-ethylene glycol ether surfactant, 0.1%-0.3% of water-soluble hyperbranched polyamino acid and 0.1% of leucopeptin, with the balance being distilled water. The vaginal matrix material disclosed by the invention retains the biological integrity of the natural vaginal tissue matrix, and id remarkable in curative effects on vaginal tissue loss, pelvic floor injury and function degradation, good in anti-bacterial and anti-infective performance and safe to use.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a vaginal matrix material and a preparation method thereof. Background technique [0002] Malformation of vaginal atresia or congenital absence of vagina is usually seen in abnormal sex development or MRKH syndrome (absence of vagina syndrome), congenital absence of uterus, etc., such as AIS (androgen insensitivity syndrome) or Abnormal development of other organs will lead to loss of vaginal tissue or damage to the bottom of the disc and functional degeneration. These diseases can cause pelvic organ prolapse, which is a common disease in obstetrics and gynecology. While these diseases bring physical pain to patients, they also cause great mental and psychological pressure to patients, seriously affecting the health and quality of life of middle-aged and elderly women. It is of great significance to adopt effective measures to carry out targeted treatment of these two ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3633A61L27/3679A61L27/3687A61L27/3691A61L2430/22
Inventor 吴海玲王才阳李现峰
Owner 吴海玲
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