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Technology for extracting chlorophyll from spirulina

A technology of spirulina and chlorophyll, applied in the direction of organic chemistry, can solve the problems of reducing yield and biological activity, large solvent consumption, chlorophyll decomposition, etc., to improve food safety, improve yield and purity, and promote the reaction. Effect

Active Publication Date: 2019-08-23
北海生巴达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used organic solvent extraction method is time-consuming, consumes a lot of solvents, is highly toxic, destroys nutrients, is costly, and is easy to decompose chlorophyll at high temperatures, thereby reducing yield and biological activity.
At present, although there are relevant literature reports on extracting chlorophyll from Spirulina, the comprehensive development and utilization technology of Spirulina and other biomass in the prior art is still immature, and most of Spirulina and other biomass have not been fully utilized, resulting in biomass A huge waste of energy; and, because the chlorophyll in spirulina can be dissolved in organic solvents such as acetone, methanol, ethanol, ethyl acetate, and insoluble in water, so organic solvents are used to carry out spirulina algae mud during extraction and processing. Dehydration, extraction of chlorophyll, precipitation, filtration and other operations after alcohol extraction, while carotene and lutein have high solubility in organic solvents, the chlorophyll obtained by this method contains carotene and lutein, and the obtained chlorophyll Contains a certain amount of organic solvents, which will affect people's health after eating

Method used

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  • Technology for extracting chlorophyll from spirulina

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A process for extracting chlorophyll from spirulina, comprising the following steps:

[0041] (1) Raw material pretreatment: Wash the fresh spirulina, treat it in the dark at 1°C for 80 minutes, then crush it at 8°C until it passes through a 50-mesh sieve, put it into a centrifuge tube with steel balls, pour it into liquid nitrogen, When the liquid nitrogen has just evaporated, cover the centrifuge tube cover and place it on a vortex instrument to vortex until the spirulina powder passes through a 250 mesh sieve; during the vortex process, repeatedly invert the centrifuge tube 3 times;

[0042] (2) Soaking: under the conditions of darkness and argon or nitrogen protection, add deionized water to the above-mentioned spirulina powder for immersion treatment, and use microwave and ultrasonic treatment to assist immersion to obtain an aqueous solution of spirulina;

[0043] The method of microwave and ultrasonic treatment assisted soaking is as follows: firstly conduct micr...

Embodiment 2

[0049] A process for extracting chlorophyll from spirulina, comprising the following steps:

[0050] (1) Raw material pretreatment: Wash the fresh spirulina, treat it in the dark at 9°C for 90 minutes, then crush it at 1°C until it passes through a 100-mesh sieve, put it into a centrifuge tube with steel balls, pour it into liquid nitrogen, When the liquid nitrogen has just evaporated, cover the centrifuge tube cover and place it on a vortex instrument to vortex until the spirulina powder passes through a 350 mesh sieve; during the vortex process, repeatedly invert the centrifuge tube 5 times;

[0051] (2) Soaking: under the conditions of darkness and argon or nitrogen protection, add deionized water to the above-mentioned spirulina powder for immersion treatment, and use microwave and ultrasonic treatment to assist immersion to obtain an aqueous solution of spirulina;

[0052] The microwave and ultrasonic treatment assisted soaking method is as follows: firstly carry out micr...

Embodiment 3

[0058] A process for extracting chlorophyll from spirulina, comprising the following steps:

[0059] (1) Raw material pretreatment: Wash the fresh spirulina, treat it in the dark at 2°C for 82 minutes, then crush it at 7°C until it passes through a 60-mesh sieve, put it into a centrifuge tube with steel balls, pour it into liquid nitrogen, When the liquid nitrogen has just evaporated, cover the centrifuge tube cover and place it on a vortex instrument to vortex until the spirulina powder passes through a 270 mesh sieve; during the vortex process, repeatedly invert the centrifuge tube 5 times;

[0060] (2) Soaking: under the conditions of darkness and argon or nitrogen protection, add deionized water to the above-mentioned spirulina powder for immersion treatment, and use microwave and ultrasonic treatment to assist immersion to obtain an aqueous solution of spirulina;

[0061] The method of microwave and ultrasonic treatment assisted soaking is as follows: firstly carry out mi...

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Abstract

The invention discloses a technology for extracting chlorophyll from spirulina. The technology for extracting the chlorophyll from the spirulina comprises the steps of raw material pre-treatment, soaking, enzymolysis, filtering and drying. According to the technology for extracting the chlorophyll from the spirulina, when the chlorophyll is extracted from the spirulina, modes of soaking, enzymolysis, microwaves and ultrasonic treatment are adopted for obtaining the chlorophyll, and use of organic solvents is avoided, so that the food safety of the chlorophyll is improved. Enzymes of protease,cellulase, pectinase, lignase, amylase, esterase and the like are adopted for conducting enzymolysis treatment on the spirulina, substances other than the chlorophyll in the spirulina are degraded, the chlorophyll in the spirulina is not destructed, thus the chlorophyll contained in the spirulina is extracted as much as possible, and a product does not contain other impurities, so that the yield and the purity of the chlorophyll are improved, the extraction yield of the chlorophyll is higher than 99.5%, and is higher than that of a traditional solvent extraction method by 8.8% or above, and the purity of the chlorophyll is higher than 99.96%.

Description

technical field [0001] The invention belongs to the technical field, in particular to a process for extracting chlorophyll from spirulina. Background technique [0002] Spirulina (scientific name: Spirulina) is a class of low-level organisms. It has the health effects of lowering cholesterol, regulating blood sugar, enhancing the immune system, anti-oxidation, anti-fatigue, treating anemia, anti-tumor, preventing cancer and suppressing cancer. Toxic and side effects of chemotherapy, improve immune function, reduce blood lipids and other effects. Spirulina is characterized by high protein content, low fat and cellulose content, and a wide variety of vitamins. It is the food with the highest content of vitamin B12 and β-carotene. In addition, it has the highest absorbable iron content among all foods, and it is also found that it contains algae protein with anti-cancer and anti-cancer effects, as well as other large mineral elements and bioactive substances that improve the b...

Claims

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Application Information

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IPC IPC(8): C07D487/22
CPCC07D487/22
Inventor 薛命雄张玮瑲李玉芬梁振秀陈国珍
Owner 北海生巴达生物科技有限公司
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