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Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof

A technology of cryogel and alkoxy ether, which is applied in tissue engineering in biomedicine and in the field of biomaterial manufacturing, can solve the problems of difficult regulation of mechanical properties, easy degradation by organisms, unstable degradation speed, etc., and achieve controllable and reliable Effects of degradation properties, directional pore structure, and low cost of raw materials

Inactive Publication Date: 2019-08-30
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mechanical properties of natural gelatin are not easy to control, the stability is relatively poor, and it is easy to be degraded by organisms. Its mechanical properties can be enhanced by cross-linking or modification of chemical bonds, such as adding glutaraldehyde, diisocyanate, carbodiimide, etc. Cross-linking agents can enhance its stability
Glutaraldehyde is the most widely used, but it has certain biological toxicity, which limits its application in biomedicine
The use of methacrylic anhydride to modify gelatin is a relatively green and safe method. It can be polymerized by photoinitiated polymerization or redox reaction, and can be prepared into injectable gels, porous materials, films, microspheres, etc. for drug release, tissue In the fields of engineering, wound dressings, etc., there are still problems that the mechanical properties are not easy to control, and the unstable degradation rate in the organism is fast.

Method used

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  • Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof
  • Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof
  • Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof

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preparation example Construction

[0032] For the preparation method of methacrylic anhydride-modified gelatin, please refer to: Biomacromolecules 2000, 1, 31-38.

[0033] The synthesis of dendritic alkoxy ethers refers to references: Macromolecules, 2008, 41, 3659-3667.

[0034] Prepare each component into an aqueous solution in advance, alkoxy ether monomer 20% (1g / 5mL), modified gelatin 10% (500mg / 5mL), oxidant ammonium persulfate 5% (40mg / 800mL), catalyst tetramethyl ethyl Diamine 5% (40mg / 800mL). One-way freezing device: place a square copper block with a side length of 5 cm in liquid nitrogen at least half an hour in advance, and always keep one side on the liquid nitrogen liquid surface to keep the temperature of the copper block and the liquid nitrogen at a constant temperature.

[0035] The present invention proposes the following examples as further illustrations, but not to limit the protection scope of the present invention.

Embodiment 1

[0036] Example 1: The mass ratio of alkoxy ether and modified gelatin is 5:5. Preparation of gel sample: pipette 300 μL of alkoxy ether monomer solution, 600 μL of modified gelatin, 800 μL of deionized water, oxidizing agent and Each catalyst was 80 μL, and the solid content of the whole system was 6 w / v%. Add it to the polypropylene plastic abrasive mold with an inner diameter of 14 mm in turn, shake evenly and use polypropylene plastic foam to isolate the surrounding heat and quickly contact the copper surface (the mold cannot touch liquid nitrogen). After the gel system is completely frozen, transfer to -22 degrees. Obtain the gel sample of alkoxy ether: gelatin=5:5 in the refrigerator.

Embodiment 2

[0037] Example 2: Preparation of a gel sample with a mass ratio of alkoxy ether and modified gelatin of 7:3: pipette 400 μL of alkoxy ether monomer solution, 360 μL of modified gelatin, deionized water from the mother liquor of each component Add 1000 μL, 80 μL each of oxidant and catalyst to a polypropylene plastic mold with an inner diameter of 14 mm in sequence, shake evenly and use foam to insulate the surrounding heat and quickly contact the copper surface (the mold cannot touch liquid nitrogen) and transfer after the gel system is completely frozen React overnight in a refrigerator at -22 degrees to obtain a gel sample of alkoxy ether: gelatin = 7:3.

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Abstract

The invention relates to an alkoxyether dendrimer / gelatin composite cryogel, a preparation method and applications thereof. According to the present invention, a series of intelligent hydrogels with characteristics of moderate mechanical properties, oriented porous structure and temperature response are prepared by using gelatin modified with methacrylic anhydride and alkoxyether dendron with excellent temperature sensitive behavior as raw materials and by adopting a redox reaction through a one-way freezing method, wherein the pore size and the mechanical properties of the gel can be adjustedby regulating the ratio of different components and the cooling rate; the method is simple, safe and effective, and does not use organic solutions; the results of cell culture show that the gel has good adhesion to cells, the cells have high survival rate, and the growth morphology of the cells is controlled by the pore size and the morphology of the gel; and the obtained hydrogel can be used forthe simulation of cell matrixes, and can further be used in other tissue engineering and drug controlled release.

Description

[0001] Technology overview [0002] The invention belongs to the field of biological material production, and relates to an alkoxyether dendrimer / gelatin composite cryogel, a preparation method and application thereof. The invention belongs to the field of polymer hydrogel materials and also belongs to the field of tissue engineering in biomedicine. Background technique [0003] The purpose of tissue engineering is to use the principles and technologies of life sciences to construct biological tissues or support the construction of tissues and organs so as to improve their functions. Matrix plays a vital role in tissue engineering and is mainly used to maintain and promote the growth of cells or tissues. An ideal tissue engineering matrix should have the following characteristics: non-toxic, good biocompatibility, suitable mechanical properties, and no harm to surrounding tissues and organs after degradation. The matrix with a porous structure has attracted a lot of attentio...

Claims

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Application Information

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IPC IPC(8): C08F289/00C08F220/30C08J9/28C12N5/00
CPCC08F289/00C08J9/28C08J2333/14C08J2489/00C12N5/0068C12N2533/30C12N2533/54C08F220/302
Inventor 李文陈艳琼乔泽刘婷王盈秋徐碧漪夏晶张夏聪张阿方
Owner SHANGHAI UNIV
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