Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof

A technology of cryogel and alkoxy ether, which is applied in tissue engineering in biomedicine and in the field of biomaterial manufacturing, can solve the problems of difficult regulation of mechanical properties, easy degradation by organisms, unstable degradation speed, etc., and achieve controllable and reliable Effects of degradation properties, directional pore structure, and low cost of raw materials

Inactive Publication Date: 2019-08-30
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mechanical properties of natural gelatin are not easy to control, the stability is relatively poor, and it is easy to be degraded by organisms. Its mechanical properties can be enhanced by cross-linking or modification of chemical bonds, such as adding glutaraldehyde, diisocyanate, carbodiimide, etc. Cross-linking agents can enhance its stability
Glutaraldehyde is the most widely used, but it has certain biological toxicity, which limits its application in bi

Method used

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  • Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof
  • Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof
  • Alkoxyether dendrimer/gelatin composite cryogel, preparation method and applications thereof

Examples

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Example Embodiment

[0032] For the preparation method of methacrylic anhydride modified gelatin, please refer to: Biomacromolecules 2000, 1, 31-38.

[0033] For the synthesis of dendritic alkoxy ethers, refer to reference: Macromolecules, 2008, 41, 3659-3667.

[0034] Each component was prepared into an aqueous solution in advance, 20% of alkoxy ether monomer (1g / 5mL), 10% of modified gelatin (500mg / 5mL), 5% of oxidant ammonium persulfate (40mg / 800mL), catalyst tetramethyl ethyl acetate Diamine 5% (40 mg / 800 mL). One-way freezing device: Place a square copper block with a side length of 5 cm in liquid nitrogen at least half an hour in advance, always keep one side on the liquid nitrogen surface, and keep the temperature of the copper block and liquid nitrogen constant.

[0035] The present invention proposes the following examples for further illustration, but does not limit the protection scope of the present invention.

Example Embodiment

[0036] Example 1: Preparation of gel samples with a mass ratio of alkoxy ether and modified gelatin of 5:5: Pipette 300 μL of alkoxy ether monomer solution, 600 μL of modified gelatin, 800 μL of deionized water, oxidant and The catalysts were each 80 μL, and the gel-forming solid content of the whole system was 6w / v%. Add it to a polypropylene plastic abrasive tool with an inner diameter of 14 mm in turn, shake it evenly, and use polypropylene plastic foam to isolate the surrounding heat to quickly contact with the copper surface (the mold cannot be in contact with liquid nitrogen) until the gel system is completely frozen and transferred to -22 degrees A gel sample with alkoxy ether:gelatin=5:5 was obtained in the refrigerator.

Example Embodiment

[0037] Example 2: Preparation of gel samples with a mass ratio of alkoxy ether and modified gelatin of 7:3: Pipette 400 μL of alkoxy ether monomer solution, 360 μL of modified gelatin, and deionized water from the mother liquor of each component 1000 μL, 80 μL of oxidant and 80 μL of catalyst respectively were added to a polypropylene plastic mold with an inner diameter of 14 mm, shaken evenly, and quickly contact the copper surface by using the foam to isolate the surrounding heat (the mold should not be in contact with liquid nitrogen) until the gel system is completely frozen and transferred The gel sample with alkoxy ether:gelatin=7:3 can be obtained by reacting in a refrigerator at -22 degrees overnight.

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Abstract

The invention relates to an alkoxyether dendrimer/gelatin composite cryogel, a preparation method and applications thereof. According to the present invention, a series of intelligent hydrogels with characteristics of moderate mechanical properties, oriented porous structure and temperature response are prepared by using gelatin modified with methacrylic anhydride and alkoxyether dendron with excellent temperature sensitive behavior as raw materials and by adopting a redox reaction through a one-way freezing method, wherein the pore size and the mechanical properties of the gel can be adjustedby regulating the ratio of different components and the cooling rate; the method is simple, safe and effective, and does not use organic solutions; the results of cell culture show that the gel has good adhesion to cells, the cells have high survival rate, and the growth morphology of the cells is controlled by the pore size and the morphology of the gel; and the obtained hydrogel can be used forthe simulation of cell matrixes, and can further be used in other tissue engineering and drug controlled release.

Description

[0001] Technology overview [0002] The invention belongs to the field of biological material production, and relates to an alkoxyether dendrimer / gelatin composite cryogel, a preparation method and application thereof. The invention belongs to the field of polymer hydrogel materials and also belongs to the field of tissue engineering in biomedicine. Background technique [0003] The purpose of tissue engineering is to use the principles and technologies of life sciences to construct biological tissues or support the construction of tissues and organs so as to improve their functions. Matrix plays a vital role in tissue engineering and is mainly used to maintain and promote the growth of cells or tissues. An ideal tissue engineering matrix should have the following characteristics: non-toxic, good biocompatibility, suitable mechanical properties, and no harm to surrounding tissues and organs after degradation. The matrix with a porous structure has attracted a lot of attentio...

Claims

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Application Information

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IPC IPC(8): C08F289/00C08F220/30C08J9/28C12N5/00
CPCC08F289/00C08J9/28C08J2333/14C08J2489/00C12N5/0068C12N2533/30C12N2533/54C08F220/302
Inventor 李文陈艳琼乔泽刘婷王盈秋徐碧漪夏晶张夏聪张阿方
Owner SHANGHAI UNIV
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