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A drug sustained-release polypeptide hydrogel and its preparation method and application

A hydrogel and application technology, which is applied in the field of drug sustained-release polypeptide hydrogel for the treatment of ischemic kidney injury, can solve the problems of short protein half-life and easy loss of biological activity, so as to promote dedifferentiation and proliferation, and reduce kidney damage. Inflammatory response, good drug release effect

Active Publication Date: 2022-03-18
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the protein may play a role in various tissue cells in the body, systemic excessive use will lead to potential risks; and the half-life of the protein in the body is short, and it is easy to lose biological activity

Method used

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  • A drug sustained-release polypeptide hydrogel and its preparation method and application
  • A drug sustained-release polypeptide hydrogel and its preparation method and application
  • A drug sustained-release polypeptide hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Preparation of drug sustained-release polypeptide hydrogel of the present invention

[0042] According to the treatment needs of the present invention, use the hydrogel to release HGF and anti-TNF-ɑ, and determine the slow release rate according to the preliminary experimental results (therapeutic effect), experimental animals (C57 mice) and application sites (mouse kidney capsule). The interpretation system is as follows:

[0043]

[0044]1) Self-assembled short peptide KLD1R (sequence: n-KLDLKLDLKLDLR-c) was synthesized and purified by Shanghai Botai Biotechnology, 100ug dry powder was dissolved in 10μl sterile water, and prepared as a 10mg / ml stock solution, and 10μl was added to 1.5ml EP tube;

[0045] 2) Dissolve 1 μg of heparin sodium (purchased from Sigma) dry powder in 1 μl of sterile water to prepare a 1 mg / ml stock solution, and add 1 μl into the EP tube;

[0046] 3) Dissolve 0.5ugHGF dry powder (purchased from Yiqiao Shenzhou Biological Co.,...

Embodiment 2

[0049] Embodiment 2 Preparation of drug sustained-release polypeptide hydrogel of the present invention

[0050] 1) The short self-assembling peptide KLD1R (sequence: n-KLDLKLDLKLDLR-c) was synthesized and purified by Shanghai Botai Biotechnology, 50ug of dry powder was dissolved in 8μl sterile water to make a self-assembling peptide aqueous solution, and added to a 1.5ml EP tube;

[0051] 2) Dissolve 0.5ug of heparin sodium (purchased from Sigma) dry powder in 0.5 μl sterile water to make heparin sodium aqueous solution, and add it to the EP tube;

[0052] 3) Dissolve 0.3ug of HGF dry powder (purchased from Yiqiao Shenzhou Biological Co., Ltd.) in 3 μl sterile water to prepare an aqueous solution of hepatocyte growth factor, and add it to the EP tube;

[0053] 4) Take 1 μl of anti-mouse TNF-α antibody (purchased from Biolegend Company) at a concentration of 0.5 mg / ml, and add it to the EP tube;

[0054] 5) Add 7.5 μl of sterile water to the EP tube, a total of 20 μl, and pip...

Embodiment 3

[0055] Embodiment 3 Preparation of drug sustained-release polypeptide hydrogel of the present invention

[0056] 1) The short self-assembling peptide KLD1R (sequence: n-KLDLKLDLKLDLR-c) was synthesized and purified by Shanghai Botai Biotechnology Co., Ltd., 300ug of dry powder was dissolved in 15μl sterile water to make a self-assembling peptide aqueous solution, and added to a 1.5ml EP tube;

[0057] 2) Dissolve 3 μg of heparin sodium (purchased from Sigma) dry powder in 3 μl of sterile water to prepare an aqueous solution of heparin sodium, and add it to the EP tube;

[0058] 3) Dissolve 1 ug of HGF dry powder (purchased from Yiqiao Shenzhou Biological Co., Ltd.) in 10 μl sterile water to prepare an aqueous solution of hepatocyte growth factor, and add it to the EP tube;

[0059] 4) Take 5 μl of anti-mouse TNF-α antibody (purchased from Biolegend Company) at a concentration of 10 mg / ml, and add it to the EP tube;

[0060] 5) Add 7 μl of sterile water to the EP tube, a total...

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Abstract

The invention discloses a polypeptide hydrogel, which is a polypeptide hydrogel prepared by mixing self-assembled peptide aqueous solution, heparin sodium aqueous solution, hepatocyte growth factor (HGF) aqueous solution and TNF-ɑ antibody (anti-TNF-ɑ). gel. The invention also discloses the preparation method and application of the aforementioned polypeptide hydrogel. The polypeptide hydrogel of the present invention has good drug slow-release effect and renal biocompatibility. By adding heparin sodium, the purpose of sequentially releasing anti-TNF-ɑ and HGF is achieved. The polypeptide hydrogel of the present invention can effectively inhibit acute After renal injury (AKI), the apoptosis of renal tubular cells reduces the expression of NGAL, reduces the renal inflammatory response, and promotes the dedifferentiation and proliferation of renal tubular cells. Efficacy, reducing the degree of kidney damage, and promoting the repair of damaged kidneys, the clinical application prospects are good.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a drug slow-release polypeptide hydrogel for treating ischemic kidney injury. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) is a common clinical emergency characterized by a rapid decline in renal function, eventually leading to acute renal failure and even failure of other organs. There are many triggering factors of AKI, such as ischemia / reperfusion, application of surgical contrast medium, inappropriate medication, rhabdomyolysis, infection, etc. In recent years, the incidence of AKI has been increasing, and the incidence of AKI in hospitalized patients has reached 1%-5%, and it is increasing rapidly. At present, in the course of clinical treatment, there is still a lack of effective drugs for the prevention and treatment of AKI. After the occurrence of AKI, although the patient's renal function has been improved by conventional treatment, it...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K9/06A61K31/727A61K38/18A61K39/395A61K47/64A61P13/12
CPCC07K7/08A61K38/1833A61K31/727A61K39/3955A61K9/06A61K2300/00
Inventor 刘敬平刘书云陆燕蓉程惊秋陈又南
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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