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Modified glassy carbon electrode for detecting dopamine and adrenaline simultaneously, preparation method and application thereof

A glassy carbon electrode and epinephrine technology, applied in the field of electrochemical analysis and detection, can solve the problems of bare electrode detection, complex pretreatment and operation steps, and few, etc., to promote electron transfer ability, increase enrichment degree, and activity The effect of many sites

Active Publication Date: 2019-09-20
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection methods for dopamine and epinephrine include gas chromatography, liquid chromatography, fluorescence method, photometric method, etc. However, these detection methods require complicated pretreatment and operation steps
Electrochemical methods have the advantages of fast operation, high precision, high sensitivity, and good selectivity, but bare electrodes are still somewhat inadequate for their detection
Chemically modified electrodes, especially polymer modified electrodes, have been widely used in analytical chemistry due to their large concentration of active groups and strong electrochemical response signals, which can improve the sensitivity of the measurement and have good stability. Most of the current research is on a single component The determination of multi-component determination is still rare, and the simultaneous determination of dopamine and adrenaline by preparing drop-coated graphene oxide polymerized eosin modified electrodes has not been reported.

Method used

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  • Modified glassy carbon electrode for detecting dopamine and adrenaline simultaneously, preparation method and application thereof
  • Modified glassy carbon electrode for detecting dopamine and adrenaline simultaneously, preparation method and application thereof
  • Modified glassy carbon electrode for detecting dopamine and adrenaline simultaneously, preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Comparative experiment of electrochemical response of prepared graphene oxide / eosin composite modified electrode

[0034] (1) Take four identical glassy carbon electrodes and use 0.3μM and 0.05μM Al 2 O 3 The slurry was polished on two polishing cloths for 5 minutes, and then washed with ultrapure water, followed by HNO 3 (1∶1), ethanol (1∶1), double distilled water ultrasonic treatment for 30s, then rinse with ultrapure water, and bake it under infrared light;

[0035] (2) Weigh 0.0290g of eosin and dissolve it in a 50mL beaker, stir with a glass rod until it is completely dissolved, and then transfer it to a 50mL volumetric flask. The solvents are all 0.1mol / L Na 2 HPO 4 With 0.1mol / L NaH 2 PO 4 The phosphate buffer solution is obtained by mixing the solution to pH 6.0, and finally the concentration of the eosin solution is 1*10 -3 mol / L;

[0036] (3) Weigh 2 mg of graphene oxide and disperse it in 2 mL of N,N-dimethylformamide, and sonicate it for 3 minutes with an ultrasoni...

Embodiment 2

[0040] Graphene oxide / eosin composite modified electrode method for detecting the concentration of dopamine and epinephrine in samples

[0041] S1. Prepare a series of concentration gradient standard solutions, put them into the working electrode, auxiliary electrode and reference electrode respectively, detect the electrochemical response signal on the working electrode, and obtain the DPV of different concentrations of dopamine and epinephrine standard solutions on the modified electrode 4 curve. Establishing a concentration-electrochemical response signal relationship according to the corresponding relationship between the electrochemical response signal and the concentration;

[0042] S2. Put the working electrode, auxiliary electrode and reference electrode in the solution of the test substance, and detect the electrochemical response signal on the working electrode,

[0043] The solution of each concentration was measured in parallel three times, and then corresponding to the ...

Embodiment 3

[0046] The influence of interfering substances in biological samples on dopamine

[0047] The modified electrode 4 responds to the current and a series of possible interference experiments.

[0048] After adding 30μM dopamine, the current response is obvious, and the response to L-cysteine, ascorbic acid, epinephrine, histamine, glucose, hydrogen peroxide (H 2 O 2 ), ethanol, tyramine, tryptamine and phenethylamine and other compounds have no obvious interference (signal change Figure 6A ), no current response is observed after adding these interfering substances, and the result shows that the modified electrode 4 has good selectivity.

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Abstract

The invention relates to the technical field of electrochemical analysis and detection, in particular to a modified glassy carbon electrode for detecting dopamine and adrenaline simultaneously, a preparation method and application thereof. The electrode has advantages of large specific surface area, high catalytic activity, and high electrical conductivity and is capable of measuring the dopamine and adrenaline simultaneously and distinguishing potentials accurately. The modified glassy carbon electrode comprises a glassy carbon electrode and a composite coating; and the composite coating is wrapped on the surface of the glassy carbon electrode and is formed by composition of graphene oxide and tetrabromofluorescein. In addition, the preparation method comprises the following steps: (1), preparing a tetrabromofluorescein solution; (2), preparing a graphene oxide dispersion solution; and (3), making a modified electrode. Besides, the application includes: S1, establishing a concentration-electrochemical response signal relationship; S2, measuring the concentration of a to-be-detected substance.

Description

Technical field [0001] The invention relates to the technical field of electrochemical analysis and detection, in particular to a modified glassy carbon electrode for simultaneous detection of dopamine and epinephrine, a preparation method and an application. Background technique [0002] Dopamine and epinephrine are important neurotransmitters in mammals, which can affect a variety of physiological processes in the organism, such as adjusting the functions of the central nervous system, kidneys, and cardiovascular systems. Abnormal levels of them in the body can cause various types of neurological diseases, such as schizophrenia, Parkinson's disease, drug and drug addiction, and AIDS. Therefore, the study of dopamine and epinephrine determination methods is of great significance. [0003] At present, the detection methods for dopamine and epinephrine include gas chromatography, liquid chromatography, fluorescence, photometry, etc. However, these detection methods require complica...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3277G01N27/48
Inventor 唐婧张炎郑胜彪朱金坤李子荣郭雨刘玉刘静
Owner ANHUI SCI & TECH UNIV
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