Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of probiotic microcapsule slowly released in intestinal tract and preparation method thereof

A technology of microcapsules and probiotics, which is applied in the directions of microcapsules, fixed on/in organic carriers, and capsule delivery, etc., can solve the problem that it is difficult to obtain microcapsules with the number of viable bacteria, the load of probiotics by sporopollenin, and probiotics. Bacterial activity reduction and other problems, to achieve good biocompatibility and safety, improve stability, stable properties

Active Publication Date: 2022-08-02
HUAZHONG AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Probiotics have a variety of health effects on the human body, such as improving intestinal function, preventing cancer, enhancing immune function, regulating cholesterol levels in the body, etc. However, many functions of probiotics are closely related to the number of viable bacteria in the large intestine and the quality of life However, most probiotic preparations have two obvious problems: (1) after passing through gastric acid, bile and other digestive juices, the activity of probiotics will decrease; (2) most probiotics are released in the stomach and small intestine, and the real Low numbers of probiotics delivered to the large intestine
[0010] However, since probiotics are living microorganisms with very special properties, it is impossible to directly use sporopollenin to load probiotics. In addition, due to the poor stability of probiotics, it is difficult to obtain microorganisms with a high number of viable bacteria by the above method. capsule

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of probiotic microcapsule slowly released in intestinal tract and preparation method thereof
  • A kind of probiotic microcapsule slowly released in intestinal tract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Embedding of Lactobacillus plantarum

[0036] The co-cultivation method was adopted: 1000 mg sunflower sporopollenin was mixed with 500 mL MRS liquid medium, sterilized at 121 °C for 15 min, cooled to room temperature and inoculated with Lactobacillus plantarum, and then propagated and cultured at 30 °C for 14 h, and the primary Microcapsules.

[0037] The primary microcapsules were placed in the simulated intestinal fluid and incubated in a constant temperature shaker at 37°C for 3.5 hours, and then the Lactobacillus plantarum in the simulated intestinal fluid was diluted, separated and counted. Simulated intestinal juice preparation method: 6.8g / L KH 2 PO 4 The pH was adjusted to 7.5±0.1 with NaOH, and 10 g / L trypsin was added after sterilization. After detection and calculation, the number of viable bacteria loaded in the primary microcapsules was 2.34×10 10 CFU / g.

[0038] (2) Preparation of bilayer microcapsules

[0039]Sodium alginate and carboxymethyl t...

Embodiment 2

[0041] (1) The primary microcapsules were prepared with lycopene, and other methods and conditions were the same as in step (1) of Example 1. After testing, the number of viable bacteria loaded was 7.28×10 6 CFU / g. The encapsulation capacity of sunflower sporopollenin for Lactobacillus plantarum was significantly greater than that of lycopene sporopollenin, which may be due to the unique structure of sunflower sporopollenin. into the cavity of the microcapsule.

Embodiment 3

[0043] (1) Prepare primary microcapsules by vacuum adsorption method: take 500mL of cultured Lactobacillus plantarum bacteria liquid, centrifuge for 10min at 3000r / min to obtain bacterial mud, dissolve in 30mL of physiological saline together with 1000mg of sunflower sporopollenin, in Under the conditions of 2 mbar and 25°C, vacuum treatment was performed for 3 h, filtered, washed and dried.

[0044] After testing, the number of live bacteria loaded is 1.32×10 2 CFU / g, basically not embedded.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
viscosityaaaaaaaaaa
viscosityaaaaaaaaaa
degree of substitutionaaaaaaaaaa
Login to View More

Abstract

The invention discloses a probiotic microcapsule slowly released in the intestinal tract, comprising a core material and a wall material, wherein the core material is a probiotic bacteria, and the wall material is composed of a first wall material coated on the outside of the core material and a wall material. It consists of a second wall material wrapped outside the first wall material, the first wall material is sporopollenin, and the second wall material is high molecular polysaccharide. The present invention can prevent the degradation and release of probiotics in the stomach, so as to reach the intestinal tract smoothly and release and absorb slowly, and at the same time, it has the advantages of good stability, high number of viable bacteria, readily available raw materials, biocompatibility and safety. Good and other advantages, has a good application prospect.

Description

technical field [0001] The invention relates to probiotic microcapsules, in particular to a probiotic microcapsule that is slowly released in the intestinal tract, and also relates to a preparation method of the microcapsules. Background technique [0002] Probiotics have a variety of health care effects on the human body, which can improve intestinal function, prevent cancer, enhance immune function, and regulate cholesterol levels in the body. However, most probiotic preparations have two obvious problems: (1) the activity of probiotics will decrease after passing through gastric acid, bile and other digestive juices; (2) most probiotics are released in the stomach and small intestine, and the real The amount of probiotics delivered to the large intestine is small. [0003] In order to overcome the above shortcomings, microencapsulation technology is often used to enhance the activity of probiotics. In recent decades, the preparation methods of microcapsules have been ext...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A23L33/135A23P10/30A23L29/00A23L29/30C12N11/10C12N11/04
CPCA23L33/135A23P10/30A23L29/03A23L29/30C12N11/10C12N11/04A23V2002/00A23V2400/169A23V2250/5026A23V2250/51
Inventor 李斌邓紫玙梁宏闪李晶宋蓉
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com