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Post-extraction method for fermentation-process epsilon-polylysine production

A polylysine and extraction method technology, which is applied in the field of post-extraction of ε-polylysine produced by fermentation, can solve the problems of large acid and alkali consumption, cumbersome extraction process, and low product purity, and achieve environmental protection cost reduction , smooth process flow and high degree of automation

Active Publication Date: 2019-11-01
山东惠仕莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, ion exchange technology has always been the core method for the extraction of ε-polylysine. As we all know, ion exchange technology has the advantages of high product recovery, strong impurity removal ability and mature operation, but its acid-base consumption in the activation and regeneration links The defects of large volume and large amount of wastewater are still unavoidable
In the production of ε-polylysine, the cost of separation and extraction accounts for 70-80% of the total production cost. The cumbersome extraction process, low yield, low product purity and high cost have always limited the industrialization of ε-polylysine. Key factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Preparation of ε-polylysine fermentation broth

[0026] Fermentation medium: glucose 60g / L, yeast extract powder 80g / L, corn steep liquor 6g / L, (NH 4 ) 2 SO 4 10g / L, K 2 HPO 4 ·7H 2 O2g / L, MgSO 4 ·7H 2 O 0.5g / L, ZnSO 4 ·7H 2 O 0.04g / L, FeSO 4 ·7H 2 O 0.05g / L, adjust the pH value to 6.8 with ammonia water;

[0027] Streptomyces albicans ( Streptomyces albus ) seed solution, inoculate the fermentation medium according to the inoculation amount of 8%, and the culture temperature is 30°C. When the pH value of the fermentation liquid drops to 4.0, the ammonia water is used to control the pH value of the fermentation liquid between 4.0-4.2 until the end of fermentation , the fermentation time is 170h;

[0028] The content of ε-polylysine in the fermentation broth prepared by the above fermentation method was 32.5 g / L.

Embodiment 2

[0029] Embodiment 2: the extraction of ε-polylysine

[0030] (1) Take 10L of the fermentation broth prepared in Example 1, add 10% HCl to adjust the pH value to 4.0, and heat it to 70°C for 30 minutes;

[0031] (2) After the pretreatment liquid is cooled to 25°C, perform solid-liquid separation, select a ceramic membrane with a pore size of 200nm, control the operating pressure at about 0.03MPa, add 2L of purified water to rinse the ceramic membrane, and obtain 11L of ceramic filtrate;

[0032] (3) Put the Tao filter hydraulic pressure into the ultrafiltration membrane system, the molecular weight cut-off of the ultrafiltration membrane is 8000D, control the temperature at 40°C, control the operating pressure at 0.1MPa, add 2L of purified water to wash the ultrafiltration membrane, and repeat the washing 3 times to obtain Ultrafiltration permeate 15.2L;

[0033] (4) Adjust the pH value of the collected ultrafiltration permeate to 6.0 with 10% NaOH, add 1% activated carbon, he...

Embodiment 3

[0038] Embodiment 3: Extraction of ε-polylysine

[0039] (1) Take 10L of the fermentation broth prepared in Example 1, add 10% HCl to adjust the pH value to 2.0, and heat it to 50°C for 10 minutes;

[0040](2) After the pretreatment liquid is cooled to 20°C, conduct solid-liquid separation, select a ceramic membrane with a pore size of 100nm, control the operating pressure at about 0.05MPa, add 4L of purified water to rinse the ceramic membrane, and obtain 13.5L of ceramic filtrate;

[0041] (3) Put the Tao filter hydraulic pressure into the ultrafiltration membrane system. The molecular weight cut-off of the ultrafiltration membrane is 20000D, the temperature is controlled at 30°C, the operating pressure is controlled at 0.12MPa, and 1L of purified water is added to rinse the ultrafiltration membrane, and the rinse is repeated 3 times to obtain Ultrafiltration permeate 15.6L;

[0042] (4) Adjust the pH value of the collected ultrafiltration permeate to 4.0 with 10% NaOH, add...

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Abstract

A post-extraction method for fermentation-process epsilon-polylysine production is provided, belonging to the technical field of extraction and separation. The method mainly includes extracting epsilon-polylysine in a fermentation solution by utilizing an electrodialysis technique and a membrane separation technique. The method specifically includes: subjecting the fermentation solution to solid liquid separation through ceramic membrane filtration after the fermentation solution is subjected to thermal flocculation; then removing soluble macromolecule substances through filtration with an ultrafiltration membrane; performing decoloration with activated carbon; performing filtration to obtain a decolorized solution; subjecting the decolorized solution to electrodialysis treatment; pumpinga dialysis liquid product to a nanofiltration membrane device and performing cyclic concentration; subjecting the concentrate to spray drying or vacuum freeze drying to obtain an epsilon-polylysine product. Compared with traditional ion exchange extraction methods, the method when being used for epsilon-polylysine extraction enables a product recovery rate of 80% or above, and product purity of 98% or above; and the method can save water by 60% and reduce the environment protection cost by 50% for products per ton after the electrodialysis technique is adopted to replace a traditional ion exchange technique. The method has advantages of capability of reducing wastewater generation, a high degree of automation, a high extraction and recovery rate and high purity of extract products, and issuitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a post-extraction method for producing ε-polylysine by fermentation. Background technique [0002] ε-polylysine (ε-PL) is an amino acid homopolymer synthesized by fermentation of microorganisms such as Streptomyces, filamentous fungi or Bacillus, which generally consists of 25-35 L-lysine monomers through α -COOH and ε-NH2 dehydration condensation, the molecular weight is usually 2500-4500D. ε-polylysine is a new type of nutritional high value-added food preservative with broad antibacterial spectrum, high biological safety, strong thermal stability and wide pH range. [0003] The preparation of ε-polylysine mainly includes two parts: microbial fermentation, extraction and refining. At present, the fermentation unit of ε-polylysine has reached the fermentation level of large-scale industrial production, and industrialization has been realized in Japan. Compared with the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/10
CPCC08G69/10
Inventor 任明刘建民孙荣张雷
Owner 山东惠仕莱生物科技有限公司
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