Pickering high internal phase emulsion, 3D printing porous support material and preparation method of Pickering high internal phase emulsion

Abstract

The invention relates to a Pickering high internal phase emulsion, a 3D printing porous support material prepared from the Pickering high internal phase emulsion and a preparation method of the Pickering high internal phase emulsion. The method comprises the following steps: nano-hydroxyapatite particles are used as an emulsion stabilizer, gelatin, collagen and genipin are dissolved into deionizedwater to form a continuous phase of an emulsion, an organic solvent is used as a dispersion phase, emulsification treatment is performed, a cross-linking reaction is performed to form the oil-in-water Pickering high internal phase emulsion, the continuous phase of the emulsion is fixed, finally 3D printing is performed to obtain a gel support, the solvent is volatilized to remove the dispersion phase, and drying is performed to obtain the porous support material with communicating pores and adjustable pore diameters. According to the method provided by the invention, an emulsion template method is adopted, the emulsion template method is combined with a 3D printing technology, so that the prepared porous support material has the advantages of high porosity, communicating pores, adjustablepore diameters, good biocompatibility, no toxicity or harm to organisms and the like, and has a wide application prospect in the field of biomedical materials.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a Pickering high internal phase emulsion, a 3D printing porous scaffold material prepared by using the same, and a preparation method. Background technique [0002] Porous scaffold material is a material with a three-dimensional network structure, which contains a large number of voids, and has unique characteristics such as large specific surface area, low density, and high porosity. field has a very wide range of applications. Therefore, it is very necessary to construct a porous scaffold material suitable for tissue repair. An ideal porous scaffold material for tissue repair should have the following characteristics: good mechanical properties to withstand environmental stress, non-toxicity to cells, good biocompatibility, drug loading, sustained release, and high porosity to secure cellular nutrients and transport of metabolic waste. [0003] At present, the...

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Problems solved by technology

[0006] 3D porous scaffold material, although the porous scaffold material prepared by this method has good biocompatibility and good mechanical properties, but polyacrylamide itself is difficult to degrade, and the porous scaffold material based on polyacrylamide needs to be removed by secondary surgery after being introduced into the human body , will cause secondary damage to the human body. In addition, acrylamide, the product of partial degradation of polyacrylamide, is toxic and harmful to the nervous system of the organism.

Chinese patent CN108201636A discloses a method for preparing a natural polymer-based 3D porous composite scaffold with controllable pore size. The Pickering emulsion template method is used, with aminated gelatin nanoparticles as a stabilizer and natural polymer as a substrate. A 3D porous composite scaffold with controllable pore size. However, this method needs to combine two desolvation methods to prepare aminated gelatin nanoparticles. The preparation process is complicated, the dialysis time period is long, and the production cost is high; During the preparation of gelatin nanoparticles, there may be residual acetone in the subsequent treatment process, which is harmful to organisms

Chinese patent CN107362392A discloses a nano-hydroxyapatite / carboxymethyl chitosan / polylactic acid glycolic acid micro-nano hybrid drug-loaded scaffold and its bionic preparation method. The method uses nano-hydroxyapatite, carboxymethyl shell The polysaccharide is the water phase, which is used as the continuous phase of the emulsion; the dichloromethane solution of polylactic acid glycolic acid is used as the oil phase, and the dispersed phase of the emulsion is used; the carboxymethyl chitosan in the continuous phase is fixed with glutaraldehyde as a crosslinking agent, Using icariin as the carrier drug, a three-dimensional biomimetic hybrid drug-loaded scaffold for bone repair was prepared by using a high-speed emulsifier combined with solvent evaporation and freeze-drying techniques. Although the drug-loaded scaffold with interconnected pores and uniform pore diameter was prepared, the drug-loaded scaffold used The cross-linking agent glutaraldehyde is toxic and harmful to organisms

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