Method for preparing cell-free medical implant material

Abstract

The invention belongs to the field of medical repair materials and relates to a method for preparing a cell-free medical implant material. In the method for preparing the cell-free materials, neitherchemically synthesized detergents nor protease products are used to remove cells; but a plant-derived nonionic surfactant is used as a decellularizing agent, thus avoiding the damage of chemical detergents, protease preparations and the like to an ECM three-dimensional structure and the loss of effective active ingredients in ECM. The cell-free medical material prepared by the method can not onlybetter maintain the three-dimensional structure of ECM, but also allow more effective active ingredients to be remained in ECM and endowed with good mechanical properties. Thereby, the ability to induce chemotaxis and differentiation of host cells is stronger, and the effect of promoting tissue repair and regeneration will be better.

Description

technical field

[0001] The invention relates to a method for preparing a medical implant material, in particular to a method for preparing a cell-free medical implant material. Background technique

[0002] Clinical implantable medical biomaterials are changing from traditional non-absorbable materials to new biomaterials that are natural, degradable, and capable of actively inducing tissue regeneration; based on the principles of tissue engineering, animal tissues are used as raw materials to remove tissue The cells in the tissue retain a relatively complete extracellular matrix (Extracellular Matrix, ECM), which is used as a raw material to prepare decellularized medical materials for tissue repair and regeneration, which is a major development direction of surgical medicine in the future.

[0003] Decellularized ECM usually has a three-dimensional structure and contains various macromolecular substances such as collagen, elastin, glycosaminoglycans (including hyaluronic a...

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